AseI from New England Biolabs

Product Specs
ItemAseI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0526L
Concentration10-50 u/µL
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

AT/TAAT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Robust regulatory interplay of enhancers, facilitators, and promoters in a native chromatin contextCellJuly 17, 2026Zhou Zhou, Yang Cheng, Jaret Lieberth, Junke Zhang, Yiyang Jin, Albert Li, Haiyuan Yu, Abdullah Ozer, John T. Lis(green),Thermo Scientific,Cat#12369010 HindIII-HF,NEB,Cat#R3104S AvrII,NEB,Cat#R0174S AseI,NEB,Cat#R0526S BbsI-HF,NEB,Cat#R3539L T4 DNA Ligase,NEB,Cat#M0202M "T4 RNA Ligase I, High Concentration",NEB,Cat#M0437M RNA 5’ Pyrophosphohydrolase (RppH

(2) Identification of New Lupane-Type Triterpenoids as Inverse Agonists of RAR-Related Orphan Receptor Gamma (RORγ)Journal of Natural ProductsAugust 22, 2025Patrik F. Schwarz, Alexander F. Perhal, Famke Guder, Jorge Enrique Hernández González, Kerrin Janssen, Ece Sağıroğlu, Ammar Tahir, Johannes Kirchmair, Natacha Rochel, Verena M. Dirsch, et al.Luna Universal qPCR Master Mix (M3003), the Monarch DNA Gel Extraction Kit (T1020S), NheI-HF (R3131S), XhoI (R0146S), AseI (R0526S), and the T4 DNA Ligase (M0202S) were purchased from New England Biolabs (NEB; Ipswich, MA, USA). Compounds

(3) A droplet digital PCR assay to measure pilin antigenic variation frequency in Neisseria gonorrhoeaemSphereMay 27, 2025Sarah J. Quillin, Di Luo, Aoife Gavagan, Arthur Prindle, H Steven Seifertand 1,000 ng of gDNA was treated with 1 µL (10 U/µL) restriction enzyme AseI overnight at 37°C (NEB R0526S), an enzyme that cuts N. gonorrhoeae DNA throughout the genome but does not cut within the pilE locus

(4) Genome-wide association study (GWAS) provides insights into the genomic basis of reproduction-related traits in Chouardia litardierei (Asparagaceae)BMC Plant BiolMay 2, 2025Sara Laura Šarančić, Nikolina Pleić, Damjan Mitić, Krešimir Križanović, Boštjan Surina, Ivan Radosavljevićwas employed [52]. In short, DNA was first digested using restriction enzymes AseI and NsiI (NEB # R0526L and # R0127L, respectively). The resulting fragments were ligated with barcoded i5 and i7 adapters,

(5) Uncovering the genomic basis of phenological traits in Chouardia litardierei (Asparagaceae) through a genome-wide association study (GWAS)Frontiers in Plant ScienceApril 17, 2025Sara Laura Šarančić, Nikolina Pleić, Krešimir Križanović, Boštjan Surina, Damjan Mitić, Ivan Radosavljević(Peterson et al., 2012). DNA was initially digested with two restriction enzymes, AseI and NsiI (NEB #R0526L and #R0127L, respectively). The resulting fragments were then ligated with barcoded i5 and i7 adapters,

(6) A Plasmid System That Utilises Phosphoribosylanthranilate Isomerase to Select Against Cells Expressing Truncated ProteinsBiomoleculesMarch 14, 2025Aditi A. Ghuge, Susanne Gottfried, Anja H. Schiemann, Evelyn Sattleggerrestriction digest mixture containing 1× buffer and 0.2 µL of AseI (2 Units, New England Biolabs, Ipswich, UK, R0526S). After overnight incubation at 37 °C, samples were resolved on a 1.8% agarose gel and bands detected

(7) The conformation of FOXM1 homodimers in vivo is crucial for regulating transcriptional activitiesNucleic Acids ResearchDecember 11, 2024Chia-Chan Hsu, Xiang Yao, Shang-Yao Chen, Tsui-Chun Tsuo, I-Ching WangEcoRI-HF restriction enzymes. FOXM1–FKBP was produced by connecting FKBP after FOXM1-YFP using AseI (#R0526, NEW ENGLAND BioLabs) and BsrGI (#R0575, NEW ENGLAND BioLabs). FRBTAD insX–FOXM1 was cloned using AgeI-HF

(8) Acetylation of WCC is dispensable for the core circadian clock but differentially regulates acute light responses in NeurosporaThe Journal of biological chemistryAugust 1, 2024Wang B, Adamo ME, Zhou X, Wang Z, Gerber SA, Kettenbach AN, Dunlap JC.mutants were targeted at the native loci. Plasmids bearing mutations were cut with AseI (NEB, Catalog # R0526S) and SspI (NEB, Catalog # R0132S) and then purified with the QIAquick PCR Purification Kit (Qiagen,

(9) Versatile generation of precise gene edits in bovines using SEGCPNBMC BiologyOctober 20, 2023Ming Wang, Fangrong Ding, Haiping Wang, Ling Li, Yunping Dai, ZhaoLin Sun, Ning Liby using Q5 (M0491S, NEB) and was cloned into the pEGFP-N1 vector (Clontech, 6085–1) by using AseI (R0526S, NEB) and KpnI (R3142S, NEB). To construct the pTmOC-S-Cre vector, the mOCT4-Cre-pA cassette was synthesized

(10) Domains required for the interaction of the central negative element FRQ with its transcriptional activator WCC within the core circadian clock of NeurosporaJournal of Biological ChemistryJuly 1, 2023Bin Wang, Jay C. Dunlapits native locus. Plasmids verified by Sanger sequencing were linearized with AseI (NEB, Catalog # R0526S) and SspI (NEB, Catalog # R0132S) and purified with the QIAquick PCR Purification Kit (Qiagen, Catalog

(11) Functional substitutions of amino acids that differ between GDF11 and GDF8 impact skeletal development and skeletal muscleLife Science AllianceMarch 1, 2023John Lian, Ryan G Walker, Andrea D’Amico, Ana Vujic, Melanie J Mills, Kathleen A Messemer, Kourtney R Mendello, Jill M Goldstein, Krystynne A Leacock, Soraya Epp, et al.TGTGGTTGGTTTGTTTGTTTGT, RV: GCCTGTGGTGCTTGAATTCA. PCR products were digested with restriction enzyme AseI (R0526; New England Biolabs), with NEBuffer 3.1, at 37°C for 20–30 min, and analyzed on 1% agarose gel. Further

(12) Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputsCell reportsDecember 6, 2022Zang Y, Chaudhari K, Bashaw GJ.,New England Biolabs,Cat#R3505S XhoI,New England Biolabs,Cat#R0146S BbsI,New England Biolabs,Cat#R3539S AseI,New England Biolabs,Cat#R0526S DpnI,New England Biolabs,Cat#R0176S T7 RNA Polymerase,Promega Corp.,Cat#P2075 10x DIG labeled-NTP,Roche,Cat#11277073910 DNAse,Roche,Cat#776785 Protein

(13) CRISPR screen to determine the in vivo fitness of Toxoplasma genesSTAR ProtocolsJune 18, 2021Lamba Omar SangarÃ©, Yifan Wang, David Arranz-SolÃs, Jeroen P.J. Saeij25 mm,Fisher,Cat#353025 24-Well plate,Fisher,Cat#353047 "AseI - 2,000 units (10,000U/mL)",NEB,Cat#R0526S "Molecular Biology Grade Water (UltraPure water, DNase free)",Corning,Cat#46-000-CV Dialysis membrane,Millipore,Cat#165-2089 5

(14) Genetic Diversity of Stellate Sturgeon in the Lower Danube River: The Impact of Habitat Contraction upon a Critically Endangered PopulationWaterApril 18, 2021Daniela Nicoleta Holostenco, Mitică Ciorpac, Elena Taflan, Katarina Tošić, Marian Paraschiv, Marian Iani, Ștefan Honț, Radu Suciu, Geta Rîșnoveanurestriction sites. Eight restriction enzymes were used to distinguish the polymorphisms: AseI (NEB R0526), RsaI (Promega R6371), HaeIII (Promega R6171), AluI (Promega R6281), Tru9I (Promega R7011), BsrI (Promega R7241),

(15) Population epigenetic divergence exceeds genetic divergence in the Eastern oyster Crassostrea virginica in the Northern Gulf of MexicoEvolutionary ApplicationsMay 1, 2020Kevin M. Johnson, Morgan W. Kellysamples in a 96‐well plate using the two frequent cutter enzymes AseI and NsiI (NEB‐R0127L and NEB‐R0526L; Van Gurp et al., 2016). Digested DNA was ligated to custom y‐yoked methylated sequencing adapters (Glenn

(16) Identification of a Master Regulator of Differentiation in ToxoplasmaCellJanuary 23, 2020Benjamin S. Waldman, Dominic Schwarz, Marc H. Wadsworth, Jeroen P. Saeij, Alex K. Shalek, Sebastian Lourido,Life Technologies,Cat#P36961 DNaseI,NEB,Cat#M0303L TURBO DNA-free kit,Thermo Fisher Scientific,Cat#AM1907 BsaI,NEB,Cat#R0535L AseI,NEB,Cat#R0526L Exonuclease I,NEB,Cat#M0293M Proteinase K,Life Technologies,Cat#25530-049 Phenol:Chloroform:Isoamyl

(17) Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genomeMobile DNADecember 1, 2019Jared P. Steranka, Zuojian Tang, Mark Grivainis, Cheng Ran Lisa Huang, Lindsay M. Payer, Fernanda O. R. Rego, Thiago Luiz Araujo Miller, Pedro A. F. Galante, Sitharam Ramaswami, Adriana Heguy, et al.Grade (Fisher Scientific, cat. no. BP2818500) (CAUTION Ethanol is highly flammable)AseI (NEB, cat. no. R0526S)BspHI (NEB, cat. no. R0517S)BstYI (NEB, cat. no. R0523S)HindIII (NEB, cat. no. R0104S)NcoI (NEB, cat.

(18) Commensal Neisseria kill Neisseria gonorrhoeae through a DNA-dependent mechanism in miceCell Host & MicrobeAugust 1, 2019Won Jong Kim, Dustin Higashi, Maira Goytia, Maria A. Rendón, Michelle Pilligua-Lucas, Matthew Bronnimann, Jeanine A. McLean, Joseph Duncan, David Trees, Ann E. Jerse, et al.R0156S NdeI,NEB,Cat# R0111S ClaI,NEB,Cat# R0197S SalI,NEB,Cat# R0138S PmeI,NEB,Cat# R0560S AseI,NEB,Cat# R0526S M.CviPI,NEB,Cat# M0227L M.SssI,NEB,Cat# M0226L DNAse I,"Invitrogen (Carlsbad, CA, USA)",Cat# 18047019 Proteinase

(19) CRISPR-Cas9-based genome-wide screening of Toxoplasma gondiiNature ProtocolsFebruary 1, 2018Saima M. Sidik, Diego Huet, Sebastian Louridoelectrocompetent cells (VWR cat. no. 95024–012)AseI and associated NEBuffer 3.1 (New England Biolabs cat. no. R0526L)DNaseI (Sigma-Aldrich cat. no. DN25–100MG)Trypsin (Worthington cat. no. LS003704)iProof and HF buffer

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AseI

AseI from New England Biolabs

Sequence

New England Biolabs

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AseI from New England Biolabs

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