AscI from New England Biolabs

Product Specs
ItemAscI
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR0558L
Quantity2500 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GG/CGCGCC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Identification of fidelity-determined residues of Porcine reproductive and respiratory syndrome virus through structural alignmentVirulenceDecember 31, 2026Xiang Gao, Junnan Zhang, Peng Gao, Xinna Ge, Yongning Zhang, Jun Han, Xin Guo, Lei Zhou, Hanchun Yangwas then cloned into the backbone plasmid, digested with restriction enzymes AscI and NheI-HF (NEB, R0558 and R3131), and assembled via homologous recombination using the ClonExpress MultiS one-step cloning

(2) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.digested with SbfI-HF (New England Biolabs, cat. no. R3642L) and AscI (New England Biolabs, cat. no. R0558S), followed by treatment with Antarctic Phosphatase (New England Biolabs, cat. no. M0289S), to linearize

(3) Visualizing suborganellar lipid distribution using correlative light and electron microscopyNature Cell BiologyMarch 20, 2026H. Mathilda Lennartz, Suman Khan, Weihua Leng, Kristin Böhlig, Gunar Fabig, Yannick Kieswald, Falk Elsner, Nadav Scher, Michaela Wilsch-Bräuninger, Ori Avinoam, et al.C-terminal of the two PABDs using BamHI (New England Biolabs, R3136S) and AscI (New England Biolabs, R0558L). The 2xPABD-mEGFP sequence was transferred into a vector for mammalian expression using NotI (New England Biolabs

(4) Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapyMolecular Therapy OncologyMarch 19, 2026Liliana Maruri-Avidal, Prajit Limsirichai, Nathaniel Gulizia, David H. Kirnthe control virus was purified40 and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630), targeting cut sites engineered into regions flanking the ΔJ2R insertion site.

(5) Poly(A) variants supporting robust transmission stability in bacteria and high protein expression in animals for mRNA in vitro transcriptionMolecular Therapy Nucleic AcidsMarch 12, 2026Hua Chen, Wei Qin, Hui Bao, Xuwei Chen, Xu Ye, Yi Wang, Liang Liu, Yanguang Zhang, Ying Sun, Tingting Zhang, et al.confirmed by Sanger sequencing at Genewiz. For ORF cloning, BamHI (NEB, Cat# R0136S) and AscI (NEB, Cat# R0558S) were used to digest the vector and insert fragments, respectively. For 3′ UTR cloning, BamHI and EcoRI

(6) Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolutioneLifeFebruary 25, 2026Alastair J Tulloch, Ryan Nicholas Delgado, Rinaldo Catta-Preta, Constance L Cepkoparent vector was linearized between the EGFP ORF and polyadenylation sequence via an AscI (NEB, cat. #R0558) recognition sequence, inserting Illumina adapter sequences flanking a recreated AscI recognition sequence,

(7) Mapping the Mutational Landscape for Streptokinase Binding to PlasminogenbioRxivFebruary 4, 2026Srishti Baid, Matthew L Holding, Taylor Laurin, David Seimeiniak, David Ginsburg, Laura M Haynesthe SK-gIIII fusion protein was mutated to a glutamine residue (CAG, Gln), between the AscI (Product# R0558S, New England Biolabs, Ipswich, MA,USA) and NotI (Product # R3189S, New England Biolabs) restriction

(8) Integrins mediate symbiont-specific uptake in cnidarian larvaeEMBO ReportsDecember 16, 2025Victor A S Jones, Melanie Dörr, Isabelle Siemers, Sebastian Rupp, Sami El Hilali, Sara Brites, Joachim M Surm, Ira Maegele, Sebastian G Gornik, Meghan Ferguson, et al.molecular cloning section. Digoxygenin-labeled probes were generated after linearization with either AscI (R0558S: NEB) or PacI (R0547S: NEB); dependent on final orientation of the probe, using DIG RNA Labeling Kit

(9) The N-terminal region of malaria vaccine candidate Plasmodium falciparum asparagine-rich merozoite antigen is immunodominant and targeted by polyreactive antibodiesbioRxivDecember 13, 2025Rolando Garza, Jeffrey M. Marchioni, Jared D. Honeycutt, Nicholas K. Hurlburt, Caroline Torres, Anakaren Garcia, Eva Loranc, Emily Yemington, Dalton Towers, Isaac Ssewanyana, et al.amplified fragments and the parent plasmid were digested using NotI-HIFI (NEB #R3189S) and AscI (NEB #R0558S) according to manufacturer specifications. The digested plasmid was run on a 1% agarose gel with gel

(10) Expression and Immunological Characterization of African Swine Fever Virus EP153R Protein for Serodiagnosis and Its Delivery via a Recombinant PRRSV Live VectorVaccines (Basel)October 29, 2025Meng Luo, Wenna Shuai, Ziqiang Guo, Jiale Li, Liwei Li, Yanjun Zhou, Yifeng Jiang, Wu Tong, Yifan Zeng, Jinbin Wang, et al.the attenuated PRRSV strain vHuN4-F112 through Asc I and EcoR V (New England Biolabs, Beijing, China, R0558/R0195) restriction sites, as described in previous studies. Recombinant clones displaying the expected

(11) DDHD2 provides a flux of saturated fatty acids for neuronal energy and functionNature MetabolismSeptember 30, 2025Saber H. Saber, Nyakuoy Yak, Xuan Ling Hilary Yong, Yih Tyng Bong, Hannah Leeson, Chuan-Yang Dai, Tobias Binder, Siyuan Lu, Reshinthine Purushothaman, An-Sofie Lenaerts, et al.FUW-DDHD2D660H-myc and FUW-DDHD2S351A-myc, the PCR fragment was digested with XbaI (NEB, R0145S) and AscI (NEB, R0558L). The primers are listed in Supplementary Table 6. Plasmid encoding the DDHD2 Trp103Arg (W103R) mutation

(12) Effects of SADS‐CoV accessory proteins NS3a, NS7a, and NS7b on viral pathogenicity: A multi‐omics investigationiMetaOmicsSeptember 1, 2025Xiaoling Yan, Xiaoli Zhang, Ling Zhou, Xiaoya Zhao, Qianniu Li, Tian Lan, Jun Fu, Jingyun Marestriction enzyme analysis, the amp‐ccdB segments of the correct clones were removed by AscI (NEB, R0558L) digestion. The resulting linear fragments were then combined with or without the fragment ΔNS7a/b‐58bp

(13) Self-splicing RNA circularization facilitated by intact group I and II intronsNat CommunAugust 10, 2025Yong Shen, Bohan Li, Lei Dong, Wei Tang, Jiwu Ren, Feng Chen, Wenjuan Zheng, Ying Yu, Lu Gao, Wensheng WeiR3539L), BsmBI-v2 (NEB, R0739L) or BsaI-HFv2 (NEB, R3733L) for CIRC and PmeI (NEB, R0560L) or AscI (NEB, R0558L) for PIE. Linearized plasmids were purified by DNA Clean & Concentrator (Zymo, D4014). Primers with

(14) Data-driven protease engineering by DNA-recording and epistasis-aware machine learningNat CommunJuly 1, 2025Lukas Huber, Tim Kucera, Simon Höllerer, Karsten Borgwardt, Sven Panke, Markus JeschekCDS and barcode or TEVs CDS and barcode were excised by restriction digest using AscI (NEB; cat. no. R0558S) and NcoI or AgeI (NEB; cat. no. R3552S) and NotI (NEB; cat. no. R3189S), respectively. Fragments were

(15) Glioblastoma-instructed astrocytes suppress tumour-specific T cell immunityNatureJuly 1, 2025Faust Akl C, Andersen BM, Li Z, Giovannoni F, Diebold M, Sanmarco LM, Kilian M, Fehrenbacher L, Pernin F, Rone JM, et al.BsaI-HF (NEB, R3535/R3733). The U6 fragment and the pLenti backbone were also digested with AscI (NEB, R0558) and SbfI-HF (NEB, R3642). The crRNA fragment was additionally digested with SbfI-HF. Digested fragments

(16) Orthogonal replication with optogenetic selection evolves yeast JEN1 into a mevalonate transporterMolecular Systems BiologyJune 11, 2025Scott A Wegner, Virginia Jiang, Jeremy D Cortez, José L Avalospolymerase,Takara,639298 Phire Plant PCR mix,Thermo,F160S T4 Ligase,NEB,M0202S XmaI,NEB,R0180S AscI,NEB,R0558S MreI,Thermo,ER2021 NotI,NEB,R0189S XhoI,NEB,R0146S DpnI,NEB,R0176S Miniprep kit,Epoch Life Science,2160-050 E.N.Z.A.

(17) Precise measurement of molecular phenotypes with barcode-based CRISPRi systemsGenome BiolMay 25, 2025Joseph H. Lobel, Nicholas T. Ingoliareporters into the barcoded libraries, 2 µg of the sgRNA-barcode library was linearized with AscI (NEB, R0558S) and, separately, 2 µg of the plasmids containing the reporters and promoters (pJL206 or pJL261) were

(18) MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samplesElifeMay 20, 2025Yasuhiro Arimura, Hide A Konishi, Hironori Funabikiprotein",MluI,New England Biolabs,R3198S, "Peptide, recombinant protein",AscI,New England Biolabs,R0558S, "Peptide, recombinant protein",XhoI,New England Biolabs,R0146S, "Chemical compound, drug",EZ-link Maleimide-PEG2-Biotin

(19) Massively parallel reporter assay for mapping gene-specific regulatory regions at single nucleotide resolutionbioRxivMay 20, 2025Alastair J Tulloch, Ryan N Delgado, Rinaldo Catta-Preta, Constance L Cepkoparent vector was linearized between the EGFP ORF and polyadenylation sequence via an AscI (NEB, cat. #R0558) recognition sequence, inserting Illumina adapter sequences flanking a recreated AscI recognition sequence,

(20) Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in riceThe EMBO JournalMay 19, 2025Lijie Liu, Jianqin Hao, Ke Huang, Penggen Duan, Baolan Zhang, Zhihai Chi, Xiaohong Yao, Yunhai Lireagents" BamH I,New England Biolabs,R3136S Pac I,New England Biolabs,R0547L Asc I,New England Biolabs,R0558S T4 DNA ligase,New England Biolabs,M0202S Aar I,Thermo Fisher Scientific,ER1581 Kpn I,New England Biolabs

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AscI

AscI from New England Biolabs

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AscI from New England Biolabs

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