Description
ScienCell's GeneQuery human neural development and regeneration qPCR array kit (GQHNDR) is designed to facilitate gene expression profiling of key genes involved in (i) human neurogenesis, migration and adhesion; (ii) neural development and differentiation; (iii) neural regeneration; as well as (iv) key genes in related degeneration diseases, such as Alzheimer's Parkinson's and Huntington's diseases. 88 genes are selected in this kit based on database and literature research. GeneQuery PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis