Description
ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit (GQH-MAD) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) adipogenic lineage commitment and differentiation. MSCs have the capacity to self-renew while maintaining stemness, and have the potential to differentiate into specialized cell lineages including adipocytes. The PPAR-gamma signaling pathway plays a key role in the induction and regulation of the adipogenesis process. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis