Description
ScienCell's GeneQuery™ Human CTLA4 Checkpoint Pathway qPCR Array Kit (GQH-CTL) is designed to facilitate gene expression profiling of 88 key genes involved in the human CTLA4 checkpoint pathway and to help identify potential prognostic biomarkers for cancer immunotherapy. CTLA4 is a negative regulator of T cell immune function. It regulates T cell priming in the initial stage of antigen stimulation. CTLA4 can antagonize CD28 signaling in T cells, as CTLA4 and CD28 compete for the same binding ligands, CD80 and CD86. Cancer immunotherapy using CTLA4 immune-checkpoint blockade (ICB) has emerged as a promising strategy to activate antitumor T cell responses, and has led to new immunotherapies for various solid and hematological tumors. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis