Description
ScienCell's GeneQuery™ Human Neural Progenitor Markers qPCR Array Kit (GQH-NPM) is designed to facilitate gene expression profiling of 40 marker genes commonly used to assess neural progenitor cell (NPC) maintenance, proliferation, and differentiation. NPCs are generated by asymmetric mitosis of neural stem cells (NSCs), in which one NSC becomes one NSC and one NPC, or by terminal asymmetric division, in which one NSC becomes two NPCs. The multipotent NPCs have limited differentiation capacity and can differentiate into 3 cell types: neurons, astrocytes and oligodendrocytes. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis