Description
ScienCell's GeneQuery™ Human Hemostasis qPCR Array Kit (GQH-HEM) profiles 88 key genes involved in stopping bleeding. Upon wounding, hemostasis generally involves vasoconstriction to prevent blood flow to the afflicted area, forming a temporary plug to inhibit bleeding, and then coagulation or blood clotting. Examples of disorders in hemostasis are hemophilia, thrombosis, and embolisms. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis