Description
ScienCell's GeneQuery™ Human Hepatitis B qPCR Array Kit (GQH-HPB) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis B pathogenesis. Hepatitis B is a liver infectious disease caused by the Hepatitis B virus (HBV). The HBV infection of hepatocytes triggers immune response cascades to regulate HBV replication and hepatocyte survival. GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis