ApeKI from New England Biolabs

Product Specs
ItemApeKI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0643L
Quantity1250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Implications of morphological and molecular distinctness on the registration of alfalfa candidate varieties issued by a breeding program: a case studyFront Plant SciApril 14, 2026Paolo Annicchiarico, Nicolò Franguelli, Luciano Pecetti, Barbara Ferrari, Nelson Nazzicari(2011), restricting the genomic DNA by the ApeKI enzyme (NEB New England Biolabs, Ipswich, MA, USA, R0643L). Modifications to the method concerned the use of 100 ng of genomic DNA and 3.6 ng of total adapters,

(2) Genetic Variation and Genome-Enabled Prediction of White Lupin Frost Resistance in Different Reference PopulationsInt J Mol SciOctober 21, 2025Nicolò Franguelli, Daniele Cavalli, Nelson Nazzicari, Luciano Pecetti, Tommaso Notario, Paolo Annicchiaricoadapters were used, and the genomic DNA was restricted with ApeKI enzyme (NEB New England Biolabs, R0643L, Ipswich, MA, USA); the library was then amplified using Kapa Taq polymerase Alpha (KAPA Library Amplification

(3) Genetic variation and genome-enabled selection of white lupin for key seed quality traitsBMC GenomicsOctober 15, 2025Paolo Annicchiarico, Claudia Osorio, Nelson Nazzicari, Barbara Ferrari, Stefania Barzaghi, Elisa Biazzi, Aldo Tava, Luciano Pecetti, Tommaso Notario, Massimo Romani, et al.3.6 ng of total adapters and restricted the genomic DNA with ApeKI enzyme (NEB New England Biolabs, R0643L); then, the library was amplified with Kapa Taq polymerase Alpha (KAPA Library Amplification Readymix,

(4) Molecular markers enhance substantially the distinctness of alfalfa varieties for registration and protectionPlant GenomeFebruary 5, 2025Paolo Annicchiarico, Nicolò Franguelli, Barbara Ferrari, Giacomo Campanella, Stefano Gualanduzzi, Margherita Crosta, Chiara Delogu, Giorgia Spataro, Nelson Nazzicariby Elshire et al. (2011), restricting the genomic DNA by the ApeKI enzyme (NEB New England Biolabs, R0643L). Modifications to the method concerned the use of 100 ng of genomic DNA and 3.6 ng of total adapters

(5) Genome-Wide Association Analysis of Yield-Related Traits and Candidate Genes in Vegetable SoybeanPlants (Basel)May 23, 2024Hongtao Gao, Guanji Wu, Feifei Wu, Xunjun Zhou, Yonggang Zhou, Keheng Xu, Yaxin Li, Wenping Zhang, Kuan Zhao, Yan Jing, et al.Beijing, China) [30]. At the same time, DNA libraries were prepared by the restriction enzyme ApeKI (R0643S, NEB, Ipswich, MA, USA) and sequenced [55,56]. At the Shenzhen Huada Gene Research Institute, we obtained

(6) The Effect of Genome Parametrization and SNP Marker Subsetting on Genomic Selection in Autotetraploid AlfalfaGenes (Basel)April 2, 2024Nelson Nazzicari, Nicolò Franguelli, Barbara Ferrari, Luciano Pecetti, Paolo Annicchiaricoadapters and restricted the genomic DNA with the ApeKI enzyme (NEB New England Biolabs, Ipswich, MA, USA, R0643L); then, the library was amplified with Kapa Taq polymerase α (KAPA Library Amplification Readymix, Kapa

(7) White Lupin Adaptation to Moderately Calcareous Soils: Phenotypic Variation and Genome-Enabled PredictionPlants (Basel)March 2, 2023Paolo Annicchiarico, Abco J. de Buck, Dimitrios N. Vlachostergios, Dennis Heupink, Avraam Koskosidis, Nelson Nazzicari, Margherita Crosta3.6 ng of total adapters, and restricted the genomic DNA with ApeKI enzyme (NEB New England Biolabs, R0643L, Ipswich, MA, USA); then, the library was amplified with Kapa Taq polymerase Alpha (KAPA Library Amplification

(8) White Lupin Drought Tolerance: Genetic Variation, Trait Genetic Architecture, and Genome-Enabled PredictionInternational Journal of Molecular SciencesJanuary 25, 2023Luciano Pecetti, Paolo Annicchiarico, Margherita Crosta, Tommaso Notario, Barbara Ferrari, Nelson Nazzicari3.6 ng of total adapters and restricted the genomic DNA with ApeKI enzyme (NEB New England Biolabs, R0643L); then, the library was amplified with Kapa Taq polymerase Alpha (KAPA Library Amplification Readymix,

(9) Evolution of selfing syndrome and its influence on genetic diversity and inbreeding: A rangeâ€wide study in Oenothera primiverisAmerican Journal of BotanyMay 21, 2022Anita Cisternasâ€Fuentes, Tania Jogesh, Geoffrey T. Broadhead, Robert A. Raguso, Krissa A. Skogen, Jeremie B. Fantharringtonii W.L. Wagner, Stockh & W.M. Klein). For the digestion, the genome was cut with ApeKI (no. R0643; New England Biolabs, Ipswich, Massachusetts, USA) and then ligated with oligonucleotides, which included

(10) Nitrogen Use Efficiency in Sorghum: Exploring Native Variability for Traits Under Variable N-RegimesFrontiers in Plant ScienceApril 21, 2021Srikanth Bollam, Kirandeep Kaur Romana, Laavanya Rayaprolu, Anilkumar Vemula, Roma Rani Das, Abhishek Rathore, Prasad Gandham, Girish Chander, Santosh P. Deshpande, Rajeev GuptaGenotyping was performed by following the GBS approach (Elshire et al., 2011), restriction enzyme ApeKI (NEB R0643L) used for complexity reduction. The GBS library was sequenced on IlluminaHiSeq 2500 (Illumina Inc.,

(11) A Genome-Wide Association Study Identifies Quantitative Trait Loci Affecting Hematological Traits in Camelus bactrianusAnimals (Basel)January 7, 2020Fucheng Guo, Liang Ming, Rendalai Si, Li Yi, Jing He, Rimutu Ji(GBS) [20,21]. The DNA was first digested using the methylation-sensitive restriction enzyme ApeKI (R0643L, New England Biolabs, Ipswich, MA, USA), and then the restriction fragments were ligated to a unique

(12) Pea genomic selection for Italian environmentsBMC GenomicsDecember 1, 2019Paolo Annicchiarico, Nelson Nazzicari, Luciano Pecetti, Massimo Romani, Luigi RussiQuant-iT™ PicoGreen® dsDNA assay kit (Life Technologies, P7589), each DNA sample (100 ng) was digested with ApeKI (NEB, R0643L) and then ligated to a unique barcoded adapter plus a common adapter. Equal volume of the ligated product

(13) Population Connectivity and Traces of Mitochondrial Introgression in New Zealand Black-Billed Gulls (Larus bulleri)Genes (Basel)November 9, 2018Claudia Mischler, Andrew Veale, Tracey van Stijn, Rudiger Brauning, John C. McEwan, Richard Maloney, Bruce C. Robertsonrestriction enzyme to use for digesting genomic DNA. For black-billed gulls, the restriction enzyme ApeKI (R0643L, New England Biolabs, Ipswich, MA, USA) was selected, and sample-specific barcode adapters were ligated

(14) Genomic Signatures of Adaptation to a Precipitation Gradient in Nigerian SorghumG3 Genes|Genomes|GeneticsOctober 1, 2018Marcus O. Olatoye, Zhenbin Hu, Fanna Maina, Geoffrey P. MorrisMorris et al. 2013). Briefly, individual DNA samples were digested using ApeKI restriction enzyme (NEB R0643L) followed by ligation of barcode and common adapters ligation using T4 DNA ligase (NEB M0202L). Ligated

(15) Population genomics of sorghum (Sorghum bicolor) across diverse agroclimatic zones of NigerGenomeApril 1, 2018Fanna Maina, Sophie Bouchet, Sandeep R. Marla, Zhenbin Hu, Jianan Wang, Aissata Mamadou, Magagi Abdou, Abdoul-Aziz Saïdou, Geoffrey P. Morrishighly differentiated genome regions (top 0.1% of SNPs). The dashed vertical lines represent a priori candidate genes. R0643L) and ligated with the barcode and common adapters using T4 DNA ligase (NEB M0202L). Ligated libraries

(16) Selection Mapping Identifies Loci Underpinning Autumn Dormancy in Alfalfa (Medicago sativa)G3 Genes|Genomes|GeneticsFebruary 1, 2018Gitanshu Munjal, Jingjie Hao, Larry R. Teuber, E. Charles Brummerpreviously in Li et al. (2014). Briefly, 100 ng of DNA from each sample was digested with ApeKI (NEB, R0643L). Two different types of adapters, a barcode adapter and a common adapter, were ligated with the DNA

(17) Genotyping-by-Sequencing and Its Exploitation for Forage and Cool-Season Grain Legume BreedingFrontiers in Plant ScienceMay 9, 2017Paolo Annicchiarico, Nelson Nazzicari, Yanling Wei, Luciano Pecetti, Edward C. Brummerwith a Quant-iT PicoGreen dsDNA assay kit, Life Technologies, P7589) was digested with ApeKI (NEB, R0643L) and then ligated to a unique barcoded adapter and a common adapter (7.0 ng of the adapter stock were

(18) A Parthenogenesis Gene Candidate and Evidence for Segmental Allopolyploidy in Apomictic Brachiaria decumbensGeneticsJuly 1, 2016Margaret Worthington, Christopher Heffelfinger, Diana Bernal, Constanza Quintero, Yeny Patricia Zapata, Juan Guillermo Perez, Jose De Vega, John Miles, Stephen Dellaporta, Joe Tohmeal. (2014) in their study of tetraploid alfalfa using the Elshire et al. (2011) protocol with ApeKI (R0643L; NEB). Over twice as many polymorphic SNPs were identiﬁed in that study; however, the mean percentage

(19) Accuracy of genomic selection for alfalfa biomass yield in different reference populationsBMC GenomicsDecember 1, 2015Paolo Annicchiarico, Nelson Nazzicari, Xuehui Li, Yanling Wei, Luciano Pecetti, E. Charles Brummerthe protocol by [19] with modifications. Briefly, 100 ng of each DNA was digested with ApeKI (NEB, R0643L) and then ligated to a unique barcoded adapter and a common adapter. Equal volume of the ligated product

(20) Mitochondrial Involvement in Vertebrate Speciation? The Case of Mito-nuclear Genetic Divergence in ChameleonsGenome Biology and EvolutionDecember 1, 2015Dan Bar-Yaacov, Zena Hadjivasiliou, Liron Levin, Gilad Barshad, Raz Zarivach, Amos Bouskila, Dan Mishmar(New England BioLabs #R0140S); ETFA—HindIII (Thermo #ER0501); and LYRM4—ApekI (New England BioLabs #R0643S). Assessing Possible Linkage between the Six nDNA Genes Which Correlated to the mtDNA Pattern It is

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ApeKI

ApeKI from New England Biolabs

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New England Biolabs

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ApeKI from New England Biolabs

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