ApaLI from New England Biolabs

Product Specs
ItemApaLI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0507S
Quantity2500 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G/TGCAC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Role of listeriolysin O and phospholipases C in L. monocytogenes intercellular protrusion dynamics, resolution, and autophagy avoidancemBioSeptember 10, 2025Madalyn Fields, Abigail Spencer, Sara J. Mason, Christopher C. Phelps, Xiaoli Zhang, Amal Amer, Stephanie Seveauenzymes BamHI,New England Biolabs,R0136S EcoRI-HF,New England Biolabs,R3101S ApaLI,New England Biolabs,R0507S KpnI-HF,New England Biolabs,R3142S EagI-HF,New England Biolabs,R3505S Plasmids,Plasmids,Plasmids Lck-mTurquoise2,Addgene

(2) Real-Time Imaging of Specific Genomic Loci With CRISPR/dCas9 in Human Cells Using CRISPRainbowBIO-PROTOCOLSeptember 5, 2025Thomas J. Versosky, Dilshodbek U. Nishonov, Li-Chun TuRestriction digest of assembled CRISPRainbow 1. ApaLI, 10,000 U/mL (New England Biolabs, catalog number: R0507S), storage: -20 °C 2. CutSmartTM buffer, 10× (New England Biolabs, catalog number: B6004S), storage:

(3) Deterministic Genetic Barcoding for Multiplexed Behavioral and Single-Cell Transcriptomic StudiesElifeFebruary 5, 2025Jorge Blanco Blanco Mendana, Margaret Donovan, Lindsey Gengelbach O’Brien, Benjamin Auch, John Garbe, Daryl M GohlMiniPrep kit,Qiagen,27104, Commercial assay or kit,ApaLI restriction enzyme,New England BioLabs (NEB),R0507S, Commercial assay or kit,PsiI restriction enzyme,NEB,R0657, Commercial assay or kit,EcoRI restriction

(4) A high-resolution two-step evolution experiment in yeast reveals a shift from pleiotropic to modular adaptationPLOS BiologyDecember 5, 2024Grant Kinsler, Yuping Li, Gavin Sherlock, Dmitri A. Petrovstrain on DNA for each sample prior to (and following) PCR amplification. We added 1 μl of ApaLI (NEB #R0507L) and 5.5 μl of Cutsmart Buffer (NEB #R0507L) to genomic DNA and incubated at 37°C for at least 1 h.

(5) Environmental memory alters the fitness effects of adaptive mutations in fluctuating environmentsNature Ecology & EvolutionJuly 17, 2024Clare I. Abreu, Shaili Mathur, Dmitri A. Petrovdigest after DNA extraction and again after the second step of the PCR, adding 1–2 μl ApaLI (NEB number R0507L) and 5 μl 10× Cutsmart to 43 μl samples and incubating at 37 °C for at least 2 h (or up to overnight). Fitness

(6) Strong environmental memory revealed by experimental evolution in static and fluctuating environmentsbioRxivSeptember 27, 2023Clare I. Abreu, Shaili Mathur, Dmitri A. Petrovperformed the digest after DNA extraction and again after the second step of PCR, adding 1–2ul ApaLI (NEB #R0507L) and 5ul 10X Cutsmart to 43-ul samples and incubating at 37°C for at least 2 hr (or up to overnight). Fitness

(7) Functional characterization of 5′ UTR cis-acting sequence elements that modulate translational efficiency in Plasmodium falciparum and humansMalaria JournalDecember 1, 2022Valentina E. Garcia, Rebekah Dial, Joseph L. DeRisiin vitro translation All mRNA generating plasmids were digested with PvuII-HF (NEB R[Δ4]151 L) and ApaLI-HF (NEB R0507L) at 37 °C for 3 h. After digestion, templates were run on a 1% agarose gel to confirm cutting and the

(8) Polymeric nanoparticles for dual-targeted theranostic gene delivery to hepatocellular carcinomaScience AdvancesJuly 22, 2022Hannah J. Vaughan et al.backbone from InvivoGen (catalog no. pcpgvtn-mcsg) with the restriction enzymes Apa LI (NEB, catalog no. R0507S) and Nhe I–HF (NEB, catalog no. R3131S) as described above. CpGf-AFP-sr39 To remove CpG dinucleotides

(9) Isolation and light chain shuffling of a Plasmodium falciparum AMA1-specific human monoclonal antibody with growth inhibitory activityMalaria JournalDecember 1, 2021Melanie Seidel-Greven et al.chains. Insert and vector sequences were double digested with the restriction endonucleases ApaLI (R0507) and NotI (R0189) and ligated with T4 DNA ligase (M0202S) purchased from NEB. A quantity of 200–500

(10) Fitness variation across subtle environmental perturbations reveals local modularity and global pleiotropy of adaptationeLifeDecember 2, 2020Grant Kinsler, Kerry Geiler-Samerotte, Dmitri A Petrovreference strain’s barcode region. We mixed 43 μL of the second step PCR product with 2 μL of ApaLI (NEB #R0507L) and 5 μL of 10X Cutsmart and incubated at 37°C for at least 2 hr (up to overnight). After digestion,

(11) GLCCI1 gene polymorphism in children with bronchial asthma: results of stage 1, single-center, open-label prospective trialImmunopathology, Allergology, InfectologyDecember 1, 2020E Minina, V Novikova, P Novikov, A BabenkoКонечный этап +72°С – 5 минут. Рестрикцию проводили с использованием фермента ApaLI (NEB, США) кат. № R0507S с CutSmart буферным раствором, согласно протоколу производителя. Для проведения 1 реакции использовали

(12) MYH 3‐associated distal arthrogryposis zebrafish model is normalized with para‐aminoblebbistatinEMBO Molecular MedicineNovember 6, 2020Julia Whittle, Lilian Antunes, Mya Harris, Zachary Upshaw, Diane S Sepich, Aaron N Johnson, Mayssa Mokalled, Lilianna Solnica‐Krezel, Matthew B Dobbs, Christina A Gurnettamplified DNA was incubated with the restriction enzyme ApaLI in CutSmart buffer (New England BioLabs; R0507S, B7204S) for 16 h at 37°C, which cleaves smyhc1 R673H mutant DNA fragments, allowing wild‐type, homozygous,

(13) Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome InstabilityMolecular CellMay 2, 2019Christopher M. Kirkham, James N.F. Scott, Xiaoling Wang, Alastair L. Smith, Adam P. Kupinski, Anthony M. Ford, David R. Westhead, Peter G. Stockley, Roman Tuma, Joan BoyesK,Roche,Cat#03115879001 NheI,NEB,Cat#R3131 ApaI,NEB,Cat#R0114 EcoRV,NEB,Cat#R0195 ApaLI,NEB,Cat#R0507 Klenow DNA polymerase,NEB,Cat#M0212 T4 DNA ligase,NEB,Cat#M0202 Vent (exo−) DNA polymerase,NEB,Cat#M0254 Taq

(14) Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodiesmAbsOctober 1, 2018Sasso E, D'Avino C, Passariello M, D'Alise AM, Siciliano D, Esposito ML, Froechlich G, Cortese R, Scarselli E, Zambrano N, et al.and BssHII (R0199) all from New England Biolabs, linearized pEU8.2VH vector, and the VLs in ApaLI (R0507) and AvrII (R0174) New England Biolabs, linearized pEU4.2VL vector. Stellar Competent Cells (Clontech

(15) Expression, Purification, and Biochemical Evaluation of Human RAD51 ProteinMethods in EnzymologyFebruary 21, 2018Shyamal Subramanyam, Maria Spies(Sigma# A8311) Ammonium Sodium Phosphate Dibasic Tetrahydrate (NaNH4PO4) (Sigma # 04266) ApaLI (NEB# R0507) Bacto™ Tryptone (BD Biosciences) Bacto™ Yeast Extract (BD Biosciences) Chicken egg white lysozyme

(16) Avoiding the Ends: Internal Epitope Tagging of Proteins Using Transposon Tn7GeneticsMay 1, 2015Rebecca E Zordan, Brian J Beliveau, Jonathan A Trow, Nancy L Craig, Brendan P CormackEtOH FseI (New England Biolabs R0588S) PmeI (New England Biolabs R0560S) ApaLI (New England Biolabs R0507S) T4 DNA ligase and buffer (New England Biolabs M0202S) Gateway LR clonase II (Life Techonologies 11791‐100) Qiagen

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ApaLI

ApaLI from New England Biolabs

Sequence

New England Biolabs

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ApaLI from New England Biolabs

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