ApaI from New England Biolabs

Product Specs
ItemApaI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0114L
Concentration20 u/µL
Quantity25000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GGGCC/C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondriaThe EMBO JournalFebruary 11, 2026Yolanda Orantos-Aguilera, Irene Sanchez-Lopez, Carlos Pascual-Caro, Patricia Gómez-Suaga, Estela Area-Gomez, Eulalia Pozo-Guisado, Jorge Montesinos, Francisco Javier Martin-Romeroreagents","Chemicals, enzymes and other reagents" Acrylamide,National Diagnostics,EC-852 ApaI,New England Biolabs,R0114 ATP,Sigma,A7699-1G BamHI,New England Biolabs,R0136 Benzamidine hydrochloride hydrate,Sigma-Aldrich,B6506-25G Blasticidin,InvivoGen,BLL-42-05 Bovine

(2) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.streptavidin-coated surface. The biotinylated DNA was digested using a KasI (NEB R0544S) or ApaI (NEB R0114S) restriction enzyme to generate single-biotin fragments of 45.68 kb and 2.82 kb, 38.41 kb and 10.090

(3) A droplet microfluidics-based platform for generating target-specific, natively-paired immune libraries and identifying potent and developable antibodiesScientific ReportsJanuary 3, 2026Anusuya Ramasubramanian, Brendan T. Deveney, Sarah A. Clark, Paula Boutin, Kayla Keepseagle, Saleh Riahi, Taotao Lao, Megan Salemi, Sagar Kathuria, Pep Amengual-Rigo, et al.°C for 4 h using 10 µL each of the enzymes MluI-HF (NEB, Cat. No. R3198L), and ApaI (NEB, Cat. No. R0114L) in a final reaction volume of 250 µL. After restriction digestion, the digested product was mixed with

(4) Depletion of tryptophanyl-tRNA synthetase and tryptophan accumulation triggers p53-dependent apoptosisCell Death DiscoveryDecember 5, 2025Tayyiba Akbar Ali, Mahmoud Izadi, Raheleh Vazehan, Maryam Al-Mansoob, Ehsan Pourkarimitph-1, and afmd-2 were cloned to the L4440 RNAi plasmid using molecular cloning and utilizing ApaI (R0114S, NEB) and NotI HF (R3189S, NEB) restriction enzyme [26]. The coding sequence of tph-1 and afmd-2 were

(5) Targeting Innate Immune Checkpoint TREX1 Is a Safe and Effective Immunotherapeutic Strategy in CancerCancer researchAugust 1, 2025Xing C, Tu X, Huai W, Tang Z, Song K, Jeltema D, Knox K, Dobbs N, Yang K, Yan N.DNase I, ssDNA was replaced by dsDNA with 3’ overhang (purified pcDNA3.1 plasmid digested with ApaI (R0114S, NEB) and KpnI-HF (R3142S, NEB)) to prepare the assay buffer. 15 nM TREX1 or 0.5 U/μl DNase I were diluted

(6) Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporterSTAR ProtocJanuary 4, 2025Yudong Teng, Kelsey Arbogast, Harald Junge, Zhe Chen,New England Biolabs,M0530S XbaI restriction enzyme,New England Biolabs,R0145L ApaI restriction enzyme,New England Biolabs,R0114L PacI restriction enzyme,New England Biolabs,R0547L EcoRI-HF restriction enzyme,New England Biolabs,R3101L RiboRuler high range RNA ladder

(7) Non-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cellsPLOS GeneticsDecember 23, 2024Eric Cheng, Ran Lu, Abigail R. Gerhold5’-TGCTTAGGGCCCCCTCTTCGATCGTCATGTTCTC-3’. The amplicon and L4440 vector were digested using ApaI (New England Biolabs, R0114S) and NcoI (New England Biolabs, R0193S) restriction enzymes. Ligation was performed using T4 DNA ligase

(8) The TRIM37 variant rs57141087 contributes to triple-negative breast cancer outcomes in Black womenEMBO ReportsNovember 29, 2024Rachisan Djiake Tihagam, Song Lou, Yuanji Zhao, Kammi Song-Yan Liu, Arjun Tushir Singh, Bon Il Koo, Piotr Przanowski, Jie Li, Xiaosong Huang, Hong Li, et al.R3142 NHEI,New England Biolabs,Cat# R3131 BAMHI,New England Biolabs,Cat# R0136S ApaI,New England Biolabs,Cat# R0114S XcmI,New England Biolabs,Cat# R0533S "Rodent Diet (2018, 625 Doxycycline)",ENVIGO,Cat# TD.01306 T4

(9) A single NLR gene confers resistance to leaf and stripe rust in wheatNature CommunicationsNovember 15, 2024Davinder Sharma, Raz Avni, Juan Gutierrez-Gonzalez, Rakesh Kumar, Hanan Sela, Manas Ranjan Prusty, Arava Shatil-Cohen, István Molnár, Kateřina Holušová, Mahmoud Said, et al.AEG-6782-2 leaves. The pγ vector was linearized with restriction enzyme ApaI (New England Biolabs, R0114S) at 25 °C for 2 h to produce a LIC cloning site. Both the linearized pγ vector and purified PCR product

(10) Matching excellence: Oxford Nanopore Technologies’ rise to parity with Pacific Biosciences in genome reconstruction of non-model bacterium with high G+C contentMicrobial GenomicsNovember 11, 2024Axel Soto-Serrano, Wenwen Li, Farhad M. Panah, Yan Hui, Pablo Atienza, Alexey Fomenkov, Richard J. Roberts, Paulina Deptula, Lukasz Krychfreudenreichii strains (TL110, TL19 and TL29) was subjected to the test with restriction enzymes ApaI and BanII (R0114 and R0119, respectively, by New England Biolabs). The reaction with BanII was assembled as follows:

(11) A large-scale cancer-specific protein–DNA interaction networkLife Science AllianceOctober 1, 2024Yunwei Lu, Anna Berenson, Ryan Lane, Isabelle Guelin, Zhaorong Li, Yilin Chen, Sakshi Shah, Meimei Yin, Luis Fernando Soto-Ugaldi, Ana Fiszbein, et al.vectors were linearized using single-cutter restriction enzymes (R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L; New England Biolabs). Table S7. Gene promoter info. The pWM#2 and pWM#3 plasmids for

(12) Combinative workflow for mRNA vaccine developmentBiochemistry and biophysics reportsSeptember 1, 2024Khanzode R, Soni G, Srivastava S, Pawar S, Wadapurkar R, Singh A.increased to 3 min for 28 cycles. Next, PCR-purified fragment AB was digested using ApaI (NEB, Cat. R0114S) and BbvCI (NEB, Cat. R0601S), CD fragment was digested using, BbvCI (NEB, Cat. R0601S) and SacII (NEB,

(13) Single-molecule imaging reveals the mechanism of bidirectional replication initiation in metazoaCellJuly 25, 2024Riki Terui, Scott E. Berger, Larissa A. Sambel, Dan Song, Gheorghe Chistolpeptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins" ApaI,NEB,Cat# R0114S BamHI-HF,NEB,Cat# R3136S BmgBI,NEB,Cat# R0628S BstXI,NEB,Cat# R0113S HindIII-HF,NEB,Cat# R3104S NcoI-HF,NEB,Cat#

(14) H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombeJournal of Biological ChemistryJune 1, 2024Bingxin Qin, Guangchun Lu, Xuejin Chen, Chenhua Zheng, Huanteng Lin, Qi Liu, Jinjie Shang, Gang Fengto the previous method (99). The plasmid pGF77 (pBluescript-leu1-3′Δ) was linearized with ApaI (NEB, R0114) to generate the integrating fragment containing 5′ homologous region and 3′ truncated homologous region

(15) Bacterial metallothionein, PmtA, a novel stress protein found on the bacterial surface of Pseudomonas aeruginosa and involved in management of oxidative stress and phagocytosismSphereMay 29, 2024Maltz-Matyschsyk M, Melchiorre CK, Knecht DA, Lynes MA.over-expressing PAO1 strain using a GFP tag, pBADPmtA was linearized using restriction enzymes ApaI (NEB R0114S) and KpnI (R3142S) following manufacturer conditions. For both WT PAO1 and DpmtA using the dTomato tag,

(16) Activity of zebrafish THAP9 transposase and zebrafish P element-like transposonsbioRxivMarch 27, 2024Nitzan Kutnowski, George E. Ghanim, Yeon Lee, Donald C. Riostock,N/A ,, "Chemicals, Enzymes and other reagents",, AhdI,New England Biolabs,R0584S ApaI,New England Biolabs,R0114S BbsI,New England Biolabs,R0539S BsaI,New England Biolabs,R3535 EcoRI,New England Biolabs,R0101S MspI,New England Biolabs

(17) Neuron cilia restrain glial KCC-3 to a microdomain to regulate multisensory processingCell ReportsMarch 26, 2024Sneha Ray, Pralaksha Gurung, R. Sean Manning, Alexandra A. Kravchuk, Aakanksha Singhvi(NEB),Cat#: R3552S EcoRI-HF,New England Biolabs (NEB),Cat#: R3101L ApaI,New England Biolabs (NEB),Cat#: R0114S BamHI-HF,New England Biolabs (NEB),Cat#: R3136L SalI-HF,New England Biolabs (NEB),Cat#: R3138L SphI-HF,New England Biolabs

(18) A universal system for boosting gene expression in eukaryotic cell-linesNat CommunMarch 16, 2024Inbal Vaknin, Or Willinger, Jonathan Mandl, Hadar Heuberger, Dan Ben-Ami, Yi Zeng, Sarah Goldberg, Yaron Orenstein, Roee Amitpurity were measured by Nanodrop. Yeast integrations We linearized purified OL plasmid by ApaI (NEB #R0114) restriction in the URA3 gene in the plasmid for yeast genomic integration at the URA3 locus. Overall,

(19) G-quadruplex in the TMV Genome Regulates Viral Proliferation and Acts as Antiviral Target of Photodynamic TherapyPLOS PathogensDecember 7, 2023Congbao Xie, Xianpeng Zhang, Wenyue Pei, Ju Sun, Hongqi Shang, Zhiyuan Huang, Mengxi Wang, Daozhong Wang, Guiqian Wang, Zhikun Gui, et al.sequence amplified using s2-3 and s3-3. The pH7LIC14−TMV−GFP was digested through restriction enzyme ApaI (R0114, NEB) and StuI (R0187, NEB) to obtain linearized vector. The sequences of TMV at 1058−4845 bp were amplified

(20) Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degenerationiScienceOctober 20, 2023Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou, et al.,New England Biolabs,Cat#R0696S I-CeuI,New England Biolabs,Cat#R0699S PacI,New England Biolabs,Cat#R0547L ApaI,New England Biolabs,Cat#R0114S MfeI-HF,New England Biolabs,Cat#R3589L XhoI,New England Biolabs,Cat#R0146S 2×RealStar Power SYBR

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ApaI

ApaI from New England Biolabs

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New England Biolabs

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ApaI from New England Biolabs

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