One-Step Antibody Biotinylation Kit from Miltenyi Biotec

(1) Microfluidic nanomagnetically isolated neuron- and astrocyte-derived extracellular vesicles to differentiate Lewy body and Alzheimer's diseaseNPJ BiosensMarch 12, 2026Stephanie J. Yang, Andrew A. Lin, Hanfei Shen, Laura W. Pappalardo, Griffin B. Spychalski, Jean Rosario, Leah K. Forsberg, Kiera M. Grant, Joshua R. Buser, Rodolfo Savica, et al.130-106-546 for GLAST, biotinylated using the One-Step Antibody Biotinylation Kit, Miltenyi Biotec, 130-093-385) on 500 µL of pooled healthy human plasma (BioIVT, HUMANPLK2-0101481). To demonstrate specificity of

(2) Differentiation-associated ISG expression of NK cells in chronic viral infectioniScienceJuly 26, 2025Franziska Keller, Robert Lorenz Chua, Timo Trefzer, Katharina Jechow, Liane Bauersfeld, Fabian Beier, Sa Sagar, Özlem Sogukpinar, Giuseppe Rusignuolo, Marta Rizzi, et al.isolation Kit,Miltenyi Biotech,Cat#130-092-657 One-Step Antibody Biotinylation Kit,Miltenyi Biotech,Cat#130-093-385 Foxp3/Transcription 442 Factor Staining Buffer,eBioscience,Cat#00-5523-00 Chromium Single Cell 3′

(3) Layilin regulates Treg motility and suppressive capacity in skinElifeJuly 21, 2025Victoire Gouirand, Sean Clancy, Courtney Macon, Jose Valle, Mariela Pauli, Hong-An Truong, Jarish Cohen, Maxime Kinet, Margaret M Lowe, Samuel J Lord, et al.and HELIOS (22F6). Layilin Ab was biotin-tagged using Miltenyi’s One-Step Antibody Biotinylation kit (130-093-385). The following anti-mouse antibodies and clones were used: CD3 (145-2C11), CD4 (RM4-5), CD8 (53-6.7),

(4) Layilin Regulates Treg Motility and Suppressive Capacity in SkinElifeJuly 21, 2025Victoire Gouirand, Sean Clancy, Courtney Macon, Jose Valle, Mariela Pauli, Hong-An Troung, Jarish Cohen, Maxime Kinet, Margaret M Lowe, Samuel J Lord, et al.and HELIOS (22F6). Layilin Ab was biotin-tagged using Miltenyi’s One-Step Antibody Biotinylation kit (130-093-385). The following anti-mouse antibodies and clones were used: CD3 (145-2C11), CD4 (RM4-5), CD8 (53-6.7),

(5) Single-mitochondrion sequencing uncovers distinct mutational patterns and heteroplasmy landscape in mouse astrocytes and neuronsBMC BiologyJuly 29, 2024Parnika S. Kadam, Zijian Yang, Youtao Lu, Hua Zhu, Yasemin Atiyas, Nishal Shah, Stephen Fisher, Erik Nordgren, Junhyong Kim, David Issadore, et al.anti-TOMM20 antibody was biotinylated via Miltenyi One-Step Antibody Biotinylation Kit (Miltenyi Biotec, 130–093-385) following the manufacturer’s protocol. Ten micrograms of anti-TOMM20 antibody was diluted to 100 μg/mL

(6) Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational lockingImmunityOctober 10, 2023Liu L, Casner RG, Guo Y, Wang Q, Iketani S, Chan JF, Yu J, Dadonaite B, Nair MS, Mohri H, et al.Lys-C,Sigma-Aldrich,Cat#1142042900 "3,3′,5,5′-tetramethylbenzidine (TMB) substrate",Sigma-Aldrich,Cat#T0440 One-Step Antibody Biotinylation Kit,Miltenyi Biotec,Cat#130-093-385 APC-streptavidin,Biolegend,Cat#405207 1 X LDS sample buffer,ThermoFisher,Cat# NP0008 ,, Deposited

(7) B-to-A transition in target DNA during retroviral integrationNucleic Acids ResearchAugust 26, 2022Ilona K Jóźwik, Wen Li, Da-Wei Zhang, Doris Wong, Julia Grawenhoff, Allison Ballandras-Colas, Sriram Aiyer, Peter Cherepanov, Alan N Engelman, Dmitry LyumkisHAB2110 antibodies that had been labelled using the One-Step Antibody Biotinylation Kit (catalog number 130-093-385) as recommended by the manufacturer (Miltenyi Biotec). Washed membranes were then incubated with streptavidin-HRP

(8) Sialylation of TLR2 initiates osteoclast fusionBone researchMarch 2, 2022Dou C, Zhen G, Dan Y, Wan M, Limjunyawong N, Cao X.Fisher Scientific). The primary antibody was biotinylated using a One-Step Antibody Biotinylation Kit (130-093-385, Miltenyi Biotec, MA) in accordance with the manufacturer’s instructions. For Siglec15+ cell separation,

(9) Layilin Anchors Regulatory T Cells in SkinJournal of immunology (Baltimore, Md. : 1950)October 1, 2021Mehta P, Gouirand V, Boda DP, Zhang J, Gearty SV, Zirak B, Lowe MM, Clancy S, Boothby I, Mahuron KM, et al.LAG3 (11C3C65). Layilin Ab was biotin-tagged using Miltenyi’s One-Step Antibody Biotinylation kit (130-093-385). Samples were run on a Fortessa analyzer (BD Biosciences) in the UCSF Flow Cytometry Core and data

(10) Layilin augments integrin activation to promote antitumor immunityThe Journal of experimental medicineSeptember 7, 2020Mahuron KM, Moreau JM, Glasgow JE, Boda DP, Pauli ML, Gouirand V, Panjabi L, Grewal R, Luber JM, Mathur AN, et al.α-layilin antibody was conjugated to biotin using the One-step Antibody Biotinylation Kit (catalog no. 130–093-385; Miltenyi Biotec) and detected with streptavidin-phycoerythrin (BioLegend). Antibodies for staining

(11) Dengue Virus Immunity Increases Zika Virus-Induced Damage during PregnancyImmunityMarch 19, 2019Brown JA, Singh G, Acklin JA, Lee S, Duehr JE, Chokola AN, Frere JJ, Hoffman KW, Foster GA, Krysztof D, et al.LightCycler 480 RNA Master Hydrolysis Probes,Roche,Cat# 4991885001 Miltenyi Biotec Biotinylation kit,Miltenyi Biotec,Order no. 130-093-385 hCGβ DuoSet ELISA kit,R&D,Cat# DY9034-05 Human Placental Lactogen ELISA kit,Alpco,Cat# 20-HPLHU-E01 RNAscope

(12) FolR1: a novel cell surface marker for isolating midbrain dopamine neural progenitors and nascent dopamine neuronsScientific reportsSeptember 1, 2016Gennet N, Tamburini C, Nan X, Li M.biotinylated FolR1 antibody prepared using the ‘One-step antibody Biotinylation kit’ (Miltenyi Biotec, 130-093-385). The labelled cells were then incubated with Anti-Biotin Microbeads for 15 minutes on ice. Cells were

(13) Peripheral Leukocyte Migration in Ferrets in Response to Infection with Seasonal Influenza VirusPloS oneJanuary 1, 2016Music N, Reber AJ, Kim JH, York IA.CD79a (clone HM47 –PerCP-Cy5.5, eBioscience). The anti-MHC class II antibody was biotinylated (cat# 130-093-385, Miltenyi Biotec, San Diego, CA) prior to use and detected with streptavidin-eFluor 450 (eBioscience). Unstained

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One-Step Antibody Biotinylation Kit from Miltenyi Biotec

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One-Step Antibody Biotinylation Kit from Miltenyi Biotec

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