Fig 2: miR-369-3p modulated the deubiquitination of NLRP3 by BRCC3. (A) Endogenous NLRP3 immunoprecipitates were analyzed for ubiquitination. miR-369-3p modulated the deubiquitination of NLRP3 by BRCC3 after miR-369-3p transient transfection and LPS and Nigericin stimulation. (B) Expression of NLRP3 and BRCC3 in BMDM lysates after miR-369-3p transient transfection and LPS and Nigericin stimulation (input samples). (C) Histograms of NLRP3 and BRCC3 expression from lysates of BMDM treated with miR-369-3p mimic and stimulated with LPS and Nigericin. To normalize data, GAPDH was used as housekeeping protein. Raw data of the independent experiments of Western blot were reported in Supplementary File S1. The histograms correspond to mean ± SEM. Data are representative of four independent experiments. (* p < 0.05; ** p < 0.01; *** p < 0.001).

Fig 3: BRCC3 and NLRP3 and ASC expression in UC patients. (A) BRCC3, NLRP3, and ASC protein expression in formalin-fixed, paraffin-embedded tissues obtained from healthy controls and ulcerative colitis (UC) patients. Original magnification, ×4. (B) Inflammatory score representing the expression levels of BRCC3, NLRP3, and ASC proteins in the immune infiltrate (* p < 0.01; *** p < 0.001).