Immunohistochemistry analysis of Collagen III was performed in human skin and testis tissue using Collagen III Polyclonal Antibody, Biotin (Product # PA1-28532) at a dilution of 1:400 for 45 minutes a room temperature. The antibody showed strong staining in FFPE sections of human skin (left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.