Western blot was performed using anti-ABCA1 Polyclonal Antibody (Product # PA1-32129) and a 254 kDa band corresponding to ABCA1 was increased upon THP-1 differentiated to macrophages and LPS treatment in THP-1. Also, the expression was observed to be high in Mouse and Rat Liver when compared to other tissues. Few uncharacterized bands were also observed across the samples tested. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 differentiated to macrophages (Lane 2), THP-1 treated with LPS (1 µg/mL for 24hrs) (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), Mouse Brown Adipose (Lane 6) and Mouse Heart (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gels (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).