RagD Antibody 4470S from Cell Signaling Technology

Product Specs
ItemRagD Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number4470S
Quantity100 µl
ApplicationsW
ReactivityH
TargetRagD
Molecular Weight50
HostRabbit
NCBI Gene AliasesHOMG7, RAGD, bA11D8.2.1
NCBI Full Gene NameRas related GTP binding D
ClonalityPolyclonal
Research AreaPI3K / Akt Signaling

Questions and Answers

Can this RagD antibody be used to study mTORC1 signaling in my experiments?

Yes, the RagD antibody has been shown to play a significant role in the regulation of mTORC1 activity. Specifically, RagD is involved in recruiting mTORC1 to lysosomes, which is crucial for its activation in response to amino acids. Studies have demonstrated that RagD promotes phosphorylation of lysosomal substrates, indicating its importance in mTORC1 signaling pathways ().

Is this antibody effective for Western blot applications?

The RagD antibody has been utilized successfully in Western blot experiments, as indicated in multiple studies. For instance, it was used at a dilution of 1:1000 in a phosphoproteomic analysis, confirming its effectiveness in detecting RagD protein levels in various cellular contexts (; ).

What species does this antibody react with?

The RagD antibody is specifically reactive with human (Homo sapiens) samples, making it suitable for studies involving human cell lines or tissues ().

Are there any publications that have cited the use of this antibody in research?

Yes, the RagD antibody has been cited in at least ten scientific articles, indicating its relevance and application in various research contexts, particularly in studies related to mTORC1 signaling and cellular responses to amino acids (; ).

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) FNIP1 Modulates B Cell Receptor Signaling Strength by Coordinating Metabolism During DevelopmentbioRxivApril 1, 2026Heon Park, Ryan Culbert, Dechen Sakya, Raynah R. Silprasert, Brian M. Iritani(Ser21/9)(9327T), Rag and LAMTOR antibody sampler kit (RagA (4357), RagB (8150), RagC (9480), RagD (4470)), Histone H3 (4499P), TFE3 (14779s), TFEB (A303-673A, bethyl lab), α-rabbit IgG HRP (W4011, promega),

(2) SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesisNat CommunOctober 3, 2025Kaushal Asrani, Adrianna Amaral, Juhyung Woo, Sanaz Nourmohammadi Abadchi, Thiago Vidotto, Eddie Imada, Alyza Skaist, Kewen Feng, Hans B. Liu, Mithila Kasbe, et al.CST; 1:1000), RAB7 (#9367 CST; 1:1000), RHEB (#13879 CST; 1:1000), RRAGC (#9480 CST; 1:1000), RRAGD (#4470 CST; 1:1000), Synaptophysin (#5461 CST; 1:1000), S6 Ribosomal Protein (#2317 CST; 1:2000), TFE3 (#14779

(3) LINC00622 transcriptionally promotes RRAGD to repress mTORC1-modulated autophagic cell death by associating with BTF3 in cutaneous melanomaCell Death DisJuly 12, 2025Can Li, Ke Wang, Lei Zhao, Jieyu Liu, Yi Jin, Chunting Zhang, Minna Xu, Min Wang, Yanjie Kuang, Jun Liu, et al.methods [45]. The following primary antibodies and dilutions were used: RRAGD (Cell Signaling Technology, 4470, 1:2000), Phospho-p70 (S6K) (Thr389) (Proteintech, 823-1-RR, 1:2000), Phospho-mTOR (Ser2448) (Proteintech,

(4) STAT6 mutations compensate for CREBBP mutations and hyperactivate IL4/STAT6/RRAGD/mTOR signaling in follicular lymphomaLeukemiaFebruary 5, 2025Qiangqiang Shao, Karan Bedi, Isabella A. Malek, Kerby Shedden, Sami N. Malek5397S), anti-phospho-STAT6 (CST # 9361S), anti-HA (CST 3724S), anti-CBP (CST, # 7389), anti-RagD (CST, # 4470), anti-HSP90 alpha/beta (Santa Cruz Biotechnology, Clone F-8, cat# sc-13119), anti-phospho-p70 S6 Kinase (Thr389

(5) Role of FLCN Phosphorylation in Insulin-Mediated mTORC1 Activation and TumorigenesisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2023Wang G, Chen L, Lei X, Qin S, Geng H, Zheng Y, Xia C, Yao J, Meng T, Deng L.Raptor (2280), SIN1 (D7G1A), FLCN (3697), TFEB (37785), WDR59 (53385), RagA (4357), RagC (9480), RagD (4470), AKT‐substrate (10001), Ki‐67 (12202S), LAMP2 (49067), Histone (4499), and E‐Cadherin (14472) were

(6) The NRF2 antagonist ML385 inhibits PI3K-mTOR signaling and growth of lung squamous cell carcinoma cellsCancer medicineMarch 1, 2023Ji L, Moghal N, Zou X, Fang Y, Hu S, Wang Y, Tsao MS.(Ser 235/236, #4858, 1:2000), 4EBP1 (#9644, 1:2000), p4EBP1 (Thr 37/46, #2855, 1:2000), and Rag D (#4470, 1:1000) (all from CST, Danvers), ß‐Actin (#A1978, 1:10000, from Sigma). 2.4 NRF2 knockdown in MGH7

(7) Brain-enriched RagB isoforms regulate the dynamics of mTORC1 activity through GATOR1 inhibitionNature cell biologySeptember 1, 2022Figlia G, Müller S, Hagenston AM, Kleber S, Roiuk M, Quast JP, Ten Bosch N, Carvajal Ibañez D, Mauceri D, Martin-Villalba A, et al.(Cell Signaling Technology #4357), RagC (Cell Signaling Technology #9480), RagD (Cell Signaling Technology #4470), phospho-S6K1 T389 (Cell Signaling Technology #9205), S6K1 (Cell Signaling Technology #2708), phospho-TFEB

(8) Phosphoproteomic Analysis of FLCN Inactivation Highlights Differential Kinase Pathways and Regulatory TFEB PhosphoserinesMolecular & cellular proteomics : MCPSeptember 1, 2022Glykofridis IE, Henneman AA, Balk JA, Goeij-de Haas R, Westland D, Piersma SR, Knol JC, Pham TV, Boekhout M, Zwartkruis FJT, et al.For Western blot experiments the following antibodies were used: FLCN (D14G9, CST 3697S, 1:1000), RRAGD (CST 4470S, 1:1000), TFE3 (CST 14779, 1:1000), TFEB (CST 3778, D2O7D; 1:3000), GAPDH (sc-47724 and MAB374; Merck Millipore

(9) A Rag GTPase dimer code defines the regulation of mTORC1 by amino acidsNature cell biologySeptember 1, 2022Gollwitzer P, Grützmacher N, Wilhelm S, Kümmel D, Demetriades C.(#14202), DYKDDDDK (FLAG) tag (#2368), mTOR (#2983), RagA (#4357), RagB (#8150), RagC (#9480), RagD (#4470), FLCN (#3697) and CTSD (#2284) proteins were purchased from Cell Signaling Technology. Anti-Raptor

(10) Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cellseLifeJanuary 18, 2021Glykofridis IE, Knol JC, Balk JA, Westland D, Pham TV, Piersma SR, Lougheed SM, Derakhshan S, Veen P, Rooimans MA, et al.3697S), Cas9 (epigentek A-9000–050), AQP1 (sc-25287), GPNMB (AF2550-SP), SQSTM1 (CST 88588), RRAGD (CST_ 4470S), FNIP1 (ab134969) FNIP2 (HPA042779), STAT2 (GTX103117), pSTAT1 Y701(7649S), TFE3 (HPA023881), H3 (9715S),

(11) Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigsJournal of applied physiology (Bethesda, Md. : 1985)February 1, 2020Suryawan A, Rudar M, Fiorotto ML, Davis TA.GATOR2/Mios (catalog no. 13557), RagA (catalog no. 4357), RagC (catalog no. 9480), RagD (catalog no. 4470), and Vps34 (catalog no. 4263) were obtained from Cell Signaling Technology (Danvers, MA) and validated

(12) Regulation of Amino Acid Transporters and Sensors in Response to a High protein Diet: A Randomized Controlled Trial in Elderly MenThe journal of nutrition, health & agingJanuary 1, 2019Zeng N, Prodhan U, D'Souza RF, Ramzan F, Mitchell SM, Sharma P, Knowles SO, Roy NC, Sjödin A, Wagner KH, et al.antibodies at 1: 1,000 dilution. The following antibodies were used: LAT1 (#5347), RagB (#8150), RagD (#4470), p70S6K1 (#2708), PRAS40 (#2691), p-p70S6K1Thr421/Ser424 (#9204), p-eIF4ESer209 (#9741), p-PRAS40Thr246

(13) EGFR is required for FOS-dependent bone tumor development via RSK2/CREB signalingEMBO molecular medicineNovember 1, 2018Linder M, Glitzner E, Srivatsa S, Bakiri L, Matsuoka K, Shahrouzi P, Dumanic M, Novoszel P, Mohr T, Langer O, et al.

(14) A LATS biosensor screen identifies VEGFR as a regulator of the Hippo pathway in angiogenesisNature communicationsMarch 13, 2018Azad T, Janse van Rensburg HJ, Lightbody ED, Neveu B, Champagne A, Ghaffari A, Kay VR, Hao Y, Shen H, Yeung B, et al.

(15) Tetraspanin CD63 Bridges Autophagic and Endosomal Processes To Regulate Exosomal Secretion and Intracellular Signaling of Epstein-Barr Virus LMP1Journal of virologyMarch 1, 2018Hurwitz SN, Cheerathodi MR, Nkosi D, York SB, Meckes DG Jr.Cell Signaling), RagA (4357; Cell Signaling), RagB (8150; Cell Signaling), RagC (5466; Cell Signaling), RagD (4470; Cell Signaling), phospho-p70 S6 kinase Thr389 (9205; Cell Signaling), p70 S6 kinase (9202; Cell Signaling),

(16) Wogonoside inhibits cell growth and induces mitochondrial-mediated autophagy-related apoptosis in human colon cancer cells through the PI3K/AKT/mTOR/p70S6K signaling pathwayOncology LettersJanuary 24, 2018Chengzheng Han, Guozheng Xing, Mengying Zhang, Min Zhong, Zhen Han, Chiyi He, Xiaoping Liu5% BSA‑TBST for 1 h at room temperature. Cells were incubated with LC3 ONCOLOGY LETTERS 15: 4463-4470, 2018 4465 secondary antibodies (cat. no. 7074; 1:5,000; Cell Signaling Technology, Inc.) for 1 h

(17) The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathwayNature communicationsSeptember 14, 2016Carbonneau M, M Gagné L, Lalonde ME, Germain MA, Motorina A, Guiot MC, Secco B, Vincent EE, Tumber A, Hulea L, et al.anti-RagB (D18F3, Cell Signaling, 1:1,000), anti-RagC (D31G9, Cell Signaling, 1:1,000), anti-RagD (4470, Cell Signaling, 1:1,000), anti-LAMTOR1 (D11H6, Cell Signaling, 1:1,000), anti-LAMTOR2 (D7C10, Cell

(18) Rab1A is an mTORC1 activator and a colorectal oncogeneCancer cellNovember 10, 2014Thomas JD, Zhang YJ, Wei YH, Cho JH, Morris LE, Wang HY, Zheng XF.Antibodies for mTOR (2983), Raptor (2280), Rheb (4935), RagA (4537), RagB (8150), RagC (5466), RagD (4470), Lamp1 (9091), Rictor, P-S6K1(T398), S6K1, P-AKT(S473), AKT, P-4E-BP1(T37/46), 4E-BP1, ERK, P-ERK(T202/Y204),

(19) Lack of Elevated Expression of TGFβ3 Contributes to the Delay of Epithelial Wound Healing in Diabetic CorneasInvestigative ophthalmology & visual scienceJanuary 1, 0001Gao N, Yu FS.System (Thermo Fisher Scientific). Antibodies to phospho-Akt (p-Akt, #9271, 1:500 dilution), p-EGFR (#4470, 1:500 dilution), and p-SMAD2/SMAD3 (#8828, 1:500 dilution) were obtained from Cell Signaling Technology

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RagD Antibody

RagD Antibody from Cell Signaling Technology

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Cell Signaling Technology

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RagD Antibody from Cell Signaling Technology

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