Fig 1: Development of multiple gene expression pigs with GT knock-out. a The newborn GT-(DAF/CD39/TFPI/C1-INH/TNFAIP3)/+ Massachusetts General Hospital (MGH) piglet delivered by Caesarean section. b Genotyping of the piglets by RT-PCR. Three of the multiple transgenic piglets were confirmed genetically with the three primer sets. PC positive control which is genomic DNA from cell line #2; NC negative control which is normal pig genomic DNA; 1, 2 and 3: genomic DNA from Knock-in pig #1, 2, and 3. c western blot analysis. The knock-in piglets expressed the multiple human genes like the constructed vector. NC negative control which is a normal pig; TG a newborn GT-(DAF/CD39/TFPI/C1-INH/TNFAIP3)/+ Massachusetts General Hospital (MGH) piglet. Red boxes represent the exact protein sizes