Immunohistochemical analysis of ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of human intestine using Sheep Anti-human ASCL2/Mash2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47852) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB (brown and counterstained with hemotoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to the base of intestinal crypts.

Western blot analysis of ASCL2 in JAR human choriocarcinoma cell line and MCF‚7 human breast cancer cell line. Samples were incubated in ASCL2 polyclonal antibody (Product # PA5-47852) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for ASCL2/Mash2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions.