Neutralization of CD254 (RANK Ligand) in mouse splenocytes. Samples were incubated in CD254 (RANK Ligand) polyclonal antibody (Product # PA5-47641). In the presence of Recombinant Mouse M‚CSF (20 ng/mL), Recombinant Mouse TRANCE/TNFSF11/RANK L induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.02-0.06 µg/mL.