Whole cell extracts of U2OS cells (40 µg) were analyzed by Western Blot using a AGO1 + AGO2 polyclonal antibody (Product # PA5-40075) at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (kDa) is shown on the left.