ChIP assays were performed on SKNO-1 cells using a RUNX1T1/ETO polyclonal antibody (Product # PA5-40064) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 µl of antibody were used per ChIP experiment. QPCR was performed on primers specific for the FUT7, NFE2, OGG1 and VEGF genes. The IP'd DNA of 6 ChIPs was pooled and analyzed with a Genome Analyzer. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.