Immunocytochemical analysis of ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47083) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Western blot analysis from lysates of mouse brain (total) tissue, mouse brain (cerebellum) tissue, and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47083) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for ALCAM/CD166 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions. ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47083) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using a 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Immunocytochemical analysis of ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47083) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.