Western blot analysis of CPSF3L (other name: IntS11 and IntS11 in Drosophila) using CPSF3L Rabbit Polyclonal Antibody (Product # PA5-46133) was performed by loading 10 mg of each cell lysates: HeLa (lane 1) and Drosophila S2 (lane 2) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 10% SDS-PAGE gel. Proteins were transferred to PVDF membrane (Product # 88518) at 30V for 60 min on ice. Membrane was blocked in 5% Milk in PBST for 30 min at room temperature. The primary CPSF3L polyclonal antibody (Product # PA5-46133) was used at a dilution of 1:500 in 5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 for 1 hr. Chemiluminescent detection was performed using ECL substrate. Several bands were detected at HeLa cells (including predicted 42 kDa band for CPSF3L) and three at Drosophila (including major predicted 68 kDa for IntS11). Data courtesy of Dr. Fondufe-Mittendorf’s lab at University of Kentucky.