Fig 1: 5mC DNA methylation changes in PFC of ZIKV offspring mice.a Identification of the DMRs in ZIKV-exposed offspring mice compared to mock controls. b Average 5mC DNA methylation levels across the gene body (top) and cortex enhancer (bottom) of all mouse genes. c, d Average 5mC DNA methylation level and genomic distribution features of gain- and loss-of 5mC regions in ZIKV offspring mice. e, f Pie charts show that the genome-wide distribution of the hypermethylated DMRs (e) or hypomethylated DMRs (f) correlates with loss of 5hmC. g GO analysis of the up-regulated genes with corresponding hypomethylated DMRs in ZIKV offspring mice. h GO analysis of the downregulated genes with corresponding hypermethylated DMRs in ZIKV offspring mice. i IGV view (upper panel) and quantification (lower panel) of 5mC levels associated with IEGs. Box–whisker plot (displaying the Max/Min at the whiskers, the 75/25 percentiles at the boxes, and the median in the center line). Egr1, n = 26 per group; Egr4, n = 25 per group; Npas4, n = 14 per group; Fos, n = 16 per group; Arc, n = 19 biological replicates per group. P values were calculated by two-tailed unpaired t-test. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.
Fig 2: Immediate-early gene (IEG) upregulation in ZIKV offspring mice.a Log2-fold change and average expression level of all genes quantified by bulk RNA-seq reads in the PFC of ZIKV offspring mice. b Gene ontology (GO) analysis for significantly upregulated genes in ZIKV-affected offspring mice. c RT-PCR analysis of IEG expression in the PFC tissues of control and ZIKV offspring mice. Box–whisker plot (displaying the Max/Min at the whiskers, the 75/25 percentiles at the boxes, and the median in the center line). n = 18 biological replicates per group. d IHC staining of c-Fos, NPAS4, and EGR1 in PFC tissues of control and ZIKV offspring mice with or without a social exposure to another mouse. Three independent experiments were repeated with similar results. Scale bar, 50 μm. e–g Quantification of the intensity of c-Fos, NPAS4, and EGR1. For e–g, n = 3-4 biological replicates per group and each data point represents one biological replicate. Data are means ± SEM. P values were calculated by two-tailed unpaired t test (c, e–g). Source data are provided as a Source Data file.
Fig 3: A possible mechanism by which RGFP966 influences SBI via the HDAC3/Npas4 pathway
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Npas4 Antibody - Azide and BSA Free
Available conjugates: Available conjugates: UnconjugatedSizes Available: 0.1 mg