Fig 1: Combination of Fasudil and SR1001 inhibited the differentiation of Th17 cells and expressions of RORγt and RhoA. (a) Percentage of Th17 cells in the spleen. (b) Expression of p-STAT3 in the spleen analyzed by Western blot. (c) Expression of RORγt in the spleen analyzed by Western blot. (d) (e) Expressions of p-STAT3, GEF-H1, RhoA and ROCK1 in the pancreatic tissues analyzed by Western blot. (f) Percentage of Th17 cells was analyzed by histogram. (g) Serum IL-17 was analyzed by ELISA. (h) Quantitative analysis of p-STAT3 in the spleen. (i) (j) (k) (l) (m) Quantitative analysis of p-STAT3, RORγt, GEF-H1, RhoA and ROCK1 in the pancreatic tissues. Values were means ± SD, n = no. of animals. **P < 0.01, #P < 0.05, ##P < 0.01.
Fig 2: SR1001 alleviated pancreatic injury and inhibited the differentiation of Th17 cells in CLP-induced sepsis model. (a) Representative HE pathological staining of pancreatic tissues section (orginal magnification × 400). (b) The histopathological scores of pancreatic injury. (c) Amylase activity in the serum was determined. (d) Apoptotic cells were examined by TUNEL analysis. (e) Immunohistochemistry analysis of RhoA in the pancreatic tissues. (f) Apoptotic cells were quantified by histogram (high power fields at × 400 magnification). (g) Relative expression level of RhoA was analyzed by histogram. (h) Expressions of GEF-H1, RhoA and ROCK1 in the pancreatic tissues analyzed by Western blot. (i) (j) (k) Quantitative analysis of GEF-H1, RhoA and ROCK1 in the pancreatic tissues. Values were means ± SD, n = no. of animals. **P < 0.01, #P < 0.05, ##P < 0.01.
Fig 3: Combination of Fasudil and SR1001 alleviated pancreatic injury in CLP-induced sepsis model. (a) Representative HE pathological staining of pancreatic tissues section (orginal magnification × 400). (b) The histopathological scores of pancreatic injury. (c) Amylase activity in the serum was determined. (d) Apoptotic cells were examined by TUNEL analysis. (e) Apoptotic cells were quantified by histogram (high power fields at × 400 magnification). (f) Immunohistochemistry analysis of RORγt in the pancreatic tissues. (g) Relative expression level of RORγt was analyzed by histogram. (h) Immunohistochemistry analysis of RhoA in the pancreatic tissues. (i) Relative expression level of RhoA was analyzed by histogram. Values were means ± SD, n = no. of animals. **P < 0.01, #P < 0.05, ##P < 0.01.
Fig 4: Combination of Fasudil and SR1001 inhibited the differentiation of Th17 cells and expression of RhoA in vitro. (a) Percentage of Th17 cells in vitro. (b) Percentage of Th17 cells was analyzed by histogram. (c) Expression of RhoA analyzed by Western blot. (d) Quantitative analysis of RhoA. Values were means ± SD, n = 6. **P < 0.01, #P < 0.05, ##P < 0.01.
Fig 5: Combination of Fasudil and SR1001 inhibited the apoptosis of pancreatic acinar cells and expression of RhoA in vitro. (a) Apoptotic cells were examined by Hoechst33342/PI staining. (b) Expression of RhoA analyzed by Western blot. (c) Apoptotic cells were quantified by histogram (high power fields at × 400 magnification). (d) Quantitative analysis of RhoA. (e) Expression of IL-17 was analyzed by ELISA. Values were means ± SD, n = 6. *P < 0.05, **P < 0.01, #P < 0.05, ##P < 0.01.
Supplier Page from Abcam for Anti-RhoA + RhoC antibody [EPR18133]