Fig 2: Analysis of RTK Phosphorylation levels. Image of RTK phosphorylation signals hybridized with cells lysate prepared from non-target transfected cells and GluIIβ knockout cells using Human RTK Phosphorylation Antibody Array Membrane (ab193662, Abcam) where 71 different anti-RTK antibodies have been spotted in duplicate, including 4 positive and 3 negative controls and 1 blank (A). Array signals were analyzed using “ImageJ software with the Protein Array Analyzer plugin16”. Values from duplicate spots were averaged and plotted (B), and the relative signal was calculated in GluIIβ knockout cells, compared to non-target transfected control cells (C).

Fig 3: Analysis of RTK Phosphorylation levels. Image of RTK phosphorylation signals hybridized with cells lysate prepared from non-target transfected cells and GluIIβ knockout cells using Human RTK Phosphorylation Antibody Array Membrane (ab193662, Abcam) where 71 different anti-RTK antibodies have been spotted in duplicate, including 4 positive and 3 negative controls and 1 blank (A). Array signals were analyzed using “ImageJ software with the Protein Array Analyzer plugin16”. Values from duplicate spots were averaged and plotted (B), and the relative signal was calculated in GluIIβ knockout cells, compared to non-target transfected control cells (C).

Fig 4: Analysis of RTK Phosphorylation levels. Image of RTK phosphorylation signals hybridized with cells lysate prepared from non-target transfected cells and GluIIβ knockout cells using Human RTK Phosphorylation Antibody Array Membrane (ab193662, Abcam) where 71 different anti-RTK antibodies have been spotted in duplicate, including 4 positive and 3 negative controls and 1 blank (A). Array signals were analyzed using “ImageJ software with the Protein Array Analyzer plugin16”. Values from duplicate spots were averaged and plotted (B), and the relative signal was calculated in GluIIβ knockout cells, compared to non-target transfected control cells (C).

Fig 5: Analysis of RTK Phosphorylation levels. Image of RTK phosphorylation signals hybridized with cells lysate prepared from non-target transfected cells and GluIIβ knockout cells using Human RTK Phosphorylation Antibody Array Membrane (ab193662, Abcam) where 71 different anti-RTK antibodies have been spotted in duplicate, including 4 positive and 3 negative controls and 1 blank (A). Array signals were analyzed using “ImageJ software with the Protein Array Analyzer plugin16”. Values from duplicate spots were averaged and plotted (B), and the relative signal was calculated in GluIIβ knockout cells, compared to non-target transfected control cells (C).