Goat Anti-Rabbit IgG H&L (Biotin) ab207995 from Abcam

Product Specs
ItemGoat Anti-Rabbit IgG H&L (Biotin)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab207995
Quantity500 µg
ClonalityPolyclonal
TargetRabbit IgG
Antibody TypePrimary Antibody
ApplicationsWB, ELISA, FCM, IHC-fr, IHC-p, IP, ICC, IF
HostGoat
IsotypeIgG
Conjugate/TagBiotin
Concentration2 mg/ml

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3 / mTORC1 Signalling in ALS MiceImmunologyNovember 6, 2025Jie Han, Xi Xu, Yan Zhao, Yao Xiao, Fei Huang, Jing Zhou, Hai Huang, Guiqian WangSOD1G93A (1:500, Abcam, UK). After washing, sections were incubated with biotinylated secondary antibody (ab207995, Abcam, UK) for 30 min at room temperature, followed by incubation with DAB substrate solution (D12384,

(2) The synergistic antitumor effects of psoralidin and cisplatin in gastric cancer by inducing ACSL4-mediated ferroptosisHereditasNovember 4, 2025Ling Yao, Jinhua Yan, Lihong Gan, Li Zheng, Peng Liu, Ling Lei, Yaqin Huangthey were rinsed with PBS and incubated for one hour with biotinylated goat anti-rabbit IgG (1:500; ab207995, Abcam) at RT. Subsequently, the sections (incubated with anti-Ki-67) were subjected to rewashing and

(3) Combining time domain modulation optofluidics and high dynamic range imaging for multiplexed, high throughput digital droplet assaysMicrosyst NanoengMay 16, 2025Yasemin Atiyas, Michael J. Siedlik, Stephanie J. Yang, David A. IssadorePleasanton, CA, USA). The biotinylated Goat Anti-Rabbit IgG secondary antibody was purchased from Abcam (ab207995, Waltham, MA, USA). The blocking buffer components, SuperBlock™ (PBS) Blocking Buffer and SuperBlock T20 (PBS) Blocking Buffer

(4) Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in miceNat CommunMay 5, 2025Xiangyun Yao, Xin Wang, Rui Zhang, Lingchi Kong, Cunyi Fan, Yun Qianprimary antibodies overnight and the biotin-conjugated secondary antibody (goat anti-rabbit, 1:500, ab207995, Abcam, USA) for 1.5 h at room temperature and DAB as substrate. For ICC analysis, mast cells were fixed

(5) Fenugreek seed extract combined with acellular nerve allografts promotes peripheral nerve regeneration and neovascularization in sciatic nerve defectsRegenerative TherapyMarch 1, 2025Yuanyuan Han, Zhiwei Liu, Chunjie Songprimary antibodies and for 30 min at 37 °C with secondary biotinylated goat anti-rabbit IgG antibody (#ab207995; 1:500; Abcam). 3,3′-diaminobenzidine (DAB) chromogenic solution was added for color development. After

(6) Mechanism of β‑sitosterol in treating keloids: Network pharmacology, molecular docking and experimental verificationMol Med RepFebruary 14, 2025Pingping Huo, Zhouna Li, Shan Jin, Sujie Wang, Yinli Luo, Lianhua Zhu, Zhehu JinThe next day, a biotinylated Goat Anti-Rabbit IgG H&L (Biotin) secondary antibody (1:100; cat. no. ab207995; Abcam) was added, and the slides were incubated at room temperature for 30 min. Streptavidin-horseradish

(7) PLEKHA4 upregulation regulates KIRC cell proliferation through β‑catenin signalingMolecular medicine reportsJanuary 1, 2025Yue Y, An G, Cao S, Li X, Du L, Xu D, Jin T, Liu L.The next day, a biotinylated Goat Anti-Rabbit IgG H&L (Biotin) secondary antibody (1:100; cat. no. ab207995; Abcam) was added, and the slides were incubated at room temperature for 30 min. Then streptavidin-horseradish

(8) The mechanism of sevoflurane affecting ovarian cancer cell proliferation and migration by regulating RNA methylase TRDMT1 to activate the β-catenin pathwayCell Biology and ToxicologyDecember 4, 2024Xiaochen Huang, Xuewei Lao, Chengyan He, Jia Wang, Ying Pan(1:250, Abcam) at 4 °C and 3 PBS rinses, and then 30-min interaction with Bio-labeled anti-IgG (1:2000, ab207995) at room temperature. Subsequently, sections were stained with freshly prepared 3,3’-diaminobenzidine

(9) CKAP2 Regulated by TFDP1 Promotes Metastasis and Proliferation of Colorectal Cancer through Affecting the Tumor MicroenvironmentJournal of Microbiology and BiotechnologyNovember 28, 2024Zhiqiang Zhong, Shi Cheng, Yang Liu1/1000) for 1 day at 4°C. Then, cells were incubated with the goat anti-rabbit secondary antibody (ab207995, Abcam, dilution: 1/1000). Ultimately, cells were stained with DAPI and a microscope was used to observe

(10) A pan-cancer analysis of EphA family gene expression and its association with prognosis, tumor microenvironment, and therapeutic targetsFrontiers in OncologyJune 17, 2024Zhe Cui, Chengwang Liu, Xuechao Wang, Yiping Xiangantibody (ab126261, Abcam; 1: 50 dilution) overnight at 4°C, and then incubated with secondary antibody (ab207995, Abcam; 1:100 dilution) followed by avidin-biotin peroxidase complex (DAKO) at room temperature for

(11) Exposure to high dose of polystyrene nanoplastics causes trophoblast cell apoptosis and induces miscarriagePart Fibre ToxicolMarch 7, 2024Shukun Wan, Xiaoqing Wang, Weina Chen, Manli Wang, Jingsong Zhao, Zhongyan Xu, Rong Wang, Chenyang Mi, Zhaodian Zheng, Huidong Zhang(Cambridge, UK). Secondary antibodies included goat anti-rabbit immunoglobulin G (IgG) (dilution 1:10,000; ab207995, Abcam) and goat anti-mouse IgG (dilution 1:10,000; ab207996, Abcam). The intensities of Western blotting

(12) Periodic changes of cyclin D1 mRNA stability are regulated by PC4 modifications in the cell cycleJournal of Cell BiologyMarch 4, 2024Qimei Pan, Peng Luo, Kaishun Hu, Yuntan Qiu, Gaoyu Liu, Shijie Dai, Bokang Cui, Dong Yin, Chunmeng ShiSigma-Aldrich) at 4°C overnight. Subsequently, the slides were incubated with a biotinylated secondary antibody (ab207995; Abcam) at 37°C for 30 min, and positive staining was visualized by using 3,3′-diaminobenzidine. RNA-seq Total

(13) Senolytic combination of dasatinib and quercetin protects against diabetic kidney disease by activating autophagy to alleviate podocyte dedifferentiation via the Notch pathwayInternational journal of molecular medicineMarch 1, 2024Zhu X, Zhang C, Liu L, Xu L, Yao L.incubation with secondary antibodies against biotin-conjugated goat anti-rabbit IgG H&L (1:500; cat. no. ab207995) for 1 h at room temperature. Following staining with diaminobenzidine at room temperature for 2-5 min

(14) Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosisJournal of ovarian researchFebruary 1, 2024Huang Q, Li Y, Chen Z, Ou H, Tan Y, Lin H.Fisher) was incubated with sections at 4 °C overnight, and the goat anti-rabbit secondary antibody (ab207995, Abcam) was also probed for 60 min. Subsequently, the slices were visualized with diaminobenzidine and

(15) Integrating Melt Electrowriting and Fused Deposition Modeling to Fabricate Hybrid Scaffolds Supportive of Accelerated Bone RegenerationAdvanced Healthcare MaterialsJanuary 1, 2024Kian F. Eichholz, Pierluca Pitacco, Ross Burdis, Farhad Chariyev‐Prinz, Xavier Barceló, Brooke Tornifoglio, Ryan Paetzold, Orquidea Garcia, Daniel J Kelly1:200 overnight at 4 °C. Next, the secondary antibody was added (Goat Anti‐Rabbit IgG H&L (Biotin), ab207995, Abcam) with a dilution of 1:250 for 1 h at room temperature, followed by rinsing with blocking buffer

(16) Triptolide protects against podocyte injury in diabetic nephropathy by activating the Nrf2/HO-1 pathway and inhibiting the NLRP3 inflammasome pathwayRenal FailureDecember 31, 2023Chenlei Lv, Tianyang Cheng, Bingbing Zhang, Ke Sun, Keda Luwere incubated for 30 min with biotin-labeled immunoglobulin G (IgG) H&L secondary antibody (1:1000, ab207995) at 37 °C. Following washing with PBS, the sections were incubated for 15 min with horseradish peroxidase

(17) The regulatory effect of the YY1/miR‑HCC2/BAMBI axis on the stemness of liver cancer cellsInternational journal of oncologyMay 1, 2023Gao H, Fan H, Xie H.Wanleibio Co., Ltd.), followed by incubation with biotinylated secondary antibodies (1:500; cat. no. ab207995; Abcam) for 1 h at room temperature. Images of the sections were captured under a light microscope (40×

(18) BPDE, the Migration and Invasion of Human Trophoblast Cells, and Occurrence of Miscarriage in Humans: Roles of a Novel lncRNA-HZ09Environmental Health PerspectivesJanuary 31, 2023Mengyuan Dai, Wenxin Huang, Xinying Huang, Chenglong Ma, Rong Wang, Peng Tian, Weina Chen, Ying Zhang, Chenyang Mi, Huidong Zhangfor 1 h. The secondary antibodies included goat anti-rabbit immunoglobulin G (IgG) (dilution 1:1,000; ab207995, Abcam) and goat antimouse IgG (dilution 1:1,000; ab207996, Abcam). The relative density of each protein

(19) The lateral superior olive in the mouse: Two systems of projecting neuronsFrontiers in Neural CircuitsOctober 20, 2022Isabella R. Williams, Anastasia Filimontseva, Catherine J. Connelly, David K. Ryugoprimary antibody. FG sections were rinsed and incubated in biotinylated goat anti-rabbit secondary (Cat #AB207995, Abcam, Cambridge, United Kingdom) and CTB sections were incubated in biotinylated rabbit anti-goat

(20) Preclinical Evaluation of Fingolimod in Rodent Models of Stroke With Age or Atherosclerosis as ComorbiditiesFrontiers in PharmacologyJuly 13, 2022Andrea C. Diaz Diaz, Kyle Malone, Jennifer A. Shearer, Anne C. Moore, Christian Waeberantibody (1:1,500; Abcam ab177487) for 1 h at RT, and with Goat Anti-Rabbit biotinylated IgG (1:250; Abcam ab207995) for 1 h at RT. Slides were washed 3 times with TBS, incubated in avidin-biotin horseradish peroxidase

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Goat Anti-Rabbit IgG H&L (Biotin) from Abcam

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Goat Anti-Rabbit IgG H&L (Biotin) from Abcam

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