RKIP (G38) Antibody 5060S from Cell Signaling Technology

Product Specs
ItemRKIP (G38) Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number5060S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk
TargetRKIP
HostRabbit
NCBI Gene AliasesHCNP, HCNPpp, HEL-210, HEL-S-34, HEL-S-96, PBP, PEBP, PEBP-1, RKIP
NCBI Full Gene Namephosphatidylethanolamine binding protein 1
ClonalityPolyclonal
Research AreaMAP Kinase Signaling
CloneG38
Molecular Weight21

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Transcriptomic Analysis Reveals an NRF2-Mediated Redox and Metabolic Reprogramming in Sorafenib-Resistant Hepatocellular Carcinoma CellsBioTech (Basel)February 11, 2026Angelo Michilli, Cristian Bassi, Farzaneh Moshiri, Bruno De Siena, Rosaria Marinaro, Elisa Callegari, Massimo Negrini, Silvia SabbioniCell Signaling Technology), and signals were visualized using Clarity Western ECL Blotting Substrate (#170-5060; Bio-Rad). Images were acquired with a ChemiDoc imaging system (Bio-Rad), and band intensities were

(2) H19 Is a PERK-Regulated Long Non-Coding RNA That Fine-Tunes UPR Signalling and Inhibits Endoplasmic Reticulum Stress-Induced Cell DeathInternational Journal of Molecular SciencesFebruary 8, 2026Wen Liu, Ananya Gupta, Michael Kerin, Sanjeev Gupta(Cell Signalling Technology, Danvers, MA, USA; Cat #9721S), β-actin (Sigma-Aldrich, St. Louis, MO, USA; Cat #A-5060), HA-Tag (Cell Signaling Technology, Danvers, MA, USA; Cat #MA1-12429), and/or Tri-methyl-histone H3

(3) Targeting metabolic vulnerabilities: REV-ERB agonist SR9009 potentiates sorafenib efficacy in liver cancerCell Death DiscoveryJanuary 19, 2026Silvia Sabbioni, Paola Guerriero, Ram C. Shankaraiah, Laura Masatti, Angelo Michilli, Cristian Bassi, Lucilla D’Abundo, Farzaneh Moshiri, Bruno De Siena, Edi Simoni, et al.Cell Signaling Technology) was used in combination with Clarity Western ECL Blotting Substrate (#170-5060; Bio-Rad), and digital images were acquired using Chemidoc (Bio-Rad). Signals were quantified with ImageJ

(4) IL-5 signaling in asthmatic derived fibroblasts exacerbates airway remodeling through ECM dysregulation and apoptosis resistanceRespiratory ResearchNovember 4, 2025Rola Abujabal, Tasneem M. Alanta, Reem Sami Alhamidi, Alaa Muayad Altaie, Lina Sahnoon, Bushra Mdkhana, Bassam Mahboub, Yves Laumonnier, Rifat Hamoudi, Khuloud Bajbouj, et al.1:500 dilutions for 1 h at room temperature. Chemiluminescence was detected using the ECL kit (Cat: 170–5060 BioRad). Protein band quantification was carried out using the Bio-Rad Image Lab software (ChemiDoc™

(5) Rapamycin rescues APC-mutated colon organoid differentiationCancer Gene TherapyJuly 23, 2025Aline Habib, Rose Mamistvalov, Dalit Ben-YosefCell Signaling, 1:5000). The blot was developed by chemiluminescence using the Clarity Western ECL substrate (170-5060, BioRad) for 1 min then exposed to X-ray film (Azure280, Azure Biosystem). Immunofluorescence (IF)

(6) CDX2 loss in colorectal cancer cells is associated with invasive properties and tumor buddingSci RepJuly 6, 2025Nils Bodmer, Kristin Uth, Rina Mehmeti, Cansaran Saygili Demir, Deborah Stroka, Nassim Ghaffari-Tabrizi-Wizsy, Alessandro Lugli, Olivier de Wever, Inti Zlobec, Mario P. Tschanhour at RT. Detection has been performed using ECL substrate (Clarity Western ECL Substrate, Cat. #170–5060; BioRad) and a ChemiDoc system (#731BR00765; BioRad). To quantify western results, protein expression

(7) Small extracellular vesicles from human bone marrow mesenchymal stromal cells enhance migration and regulate reparative gene expression in dermal fibroblastsSci RepJune 3, 2025Rui Lei, Tamiris Borges da Silva, Zhanfeng Cui, Hua Yetemperature for 60 min. The PVDF membranes were then exposed to Clarity Western ECL Substrate (Cat.: 170–5060, Bio-Rad, USA) for 10 min at room temperature and then exposed to Bio-Rad Chemidoc XRS system (Cat.:

(8) Purification of mesenchymal stromal cell-derived small extracellular vesicles using ultrafiltrationJ Extracell BiolJanuary 17, 2025Rui Lei, Shuai Ren, Hua Ye, Zhanfeng Cuitemperature. After another three times wash in PBS Tween, Clarity Western ECL Substrate (Cat.: 170–5060, Bio‐Rad, USA) was applied to the membranes for 5 min at room temperature. The results were then imaged

(9) Loss of p53 epigenetically modulates epithelial to mesenchymal transition in colorectal cancerTranslational OncologyMay 1, 2024Shreya Sharma, Harsha Rani, Yeshwanth Mahesh, Mohit Kumar Jolly, Jagannath Dixit, Vijayalakshmi MahadevanTechnology) (1:2000), before signal detection using the enhanced chemiluminescence (ECL) system (#170-5060 Biorad). The protein bands were quantified using ImageJ software [34]. Flow cytometry In order to

(10) Bridge-like lipid transfer protein family member 2 suppresses ciliogenesisMolecular biology of the cellMay 1, 2024Parolek J, Burd CG.temperature for 60 min. HRP substrates were used according to the manufacturer’s instructions (170-5060, BioRad; 34096, Thermo Fisher Scientific). Immunoblots were imaged using Molecular Imager ChemiDoc XRS+

(11) A Potent PDK4 Inhibitor for Treatment of Heart Failure with Reduced Ejection FractionCellsDecember 30, 2023Kenichi Aizawa, Akari Ikeda, Shota Tomida, Koki Hino, Yuuki Sugita, Tomoyasu Hirose, Toshiaki Sunazuka, Hiroshi Kido, Shigeyuki Yokoyama, Ryozo Nagaiperoxidase-conjugated secondary antibodies (Cell Signaling Technology) and a chemiluminescence kit (#170-5060, Bio-Rad Laboratories). Signals were detected with a ChemiDoc Touch (Bio-Rad, Hercules, CA, USA), and

(12) Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesisThe Journal of cell biologyDecember 4, 2023Kim Y, Mavodza G, Senkal CE, Burd CG.(1:10,000, 7076S; CST). HRP substrates were used according to the manufacturer's instructions (170-5060; Bio-Rad; 34096, Thermo Fisher Scientific). Blots were imaged using Molecular Imager ChemiDoc XRS+ System

(13) Age-Related Shift in Cardiac and Metabolic Phenotyping Linked to Inflammatory Cytokines and Antioxidant Status in MiceInternational Journal of Molecular SciencesOctober 31, 2023Ryeonshi Kang, Charlotte Laborde, Lesia Savchenko, Audrey Swiader, Nathalie Pizzinat, Dimitri Marsal, Yannis Sainte-Marie, Frederic Boal, Helene Tronchere, Jerome Roncalli, et al.Danvers, MA, USA). Membranes were revealed by chemiluminescence using Clarity Western ECL substrate (170-5060, BioRad, Hercules, CA, USA) on a Chemidoc Touch (BioRad). 4.6. GSH/GSSG Ratio Assay The ratio of reduced

(14) Non-glycosylated IGF2 prohormones are more mitogenic than native IGF2Communications BiologyAugust 19, 2023Pavlo Potalitsyn, Lucie Mrázková, Irena Selicharová, Michaela Tencerová, Michaela Ferenčáková, Martina Chrudinová, Tereza Turnovská, Andrzej Marek Brzozowski, Aleš Marek, Jakub Kaminský, et al.(Cell Signaling Technology #14364, dilution 1:1000). Anti-actin (20–33) antibody developed in Rabbit, Sigma A-5060, dilution 1:500. Anti-actin (20–33) antibody (Sigma, #A5060, dilution 1:500) was used as a loading control. The

(15) Chondrogenic potential of manganese-loaded composite scaffold combined with chondrocytes for articular cartilage defectJournal of Materials Science: Materials in MedicineOctober 11, 2022Li Wei, Shuai Qin, Yulin Ye, Jiawei Hu, Danyang Luo, Yusi Li, Yiming Gao, Liting Jiang, Qi Zhou, Xianfei Xie, et al.Cell Signaling, the membrane was visualized using an ECL western blotting detection system (ECL kits, #170-5060; Bio-Rad Laboratories Inc.). The results were digitized using a GE Image Quant LAS 4000 mini analyzer

(16) Cancer Cells Evade Stress-Induced Apoptosis by Promoting HSP70-Dependent Clearance of Stress GranulesCancersSeptember 25, 2022Wang A, Abulaiti X, Zhang H, Su H, Liu G, Gao S, Li L.antibody for 1 h at room temperature. The signals were detected by using the Western ECL Substrate (170-5060, Bio-Rad, Irvine, CA, USA). The images were visualized by using the Bio-Rad ChemiDoc XRS+ system. 2.6.

(17) miR-199a-3p increases the anti-tumor activity of palbociclib in liver cancer modelsMolecular Therapy - Nucleic AcidsSeptember 13, 2022Elisa Callegari, Paola Guerriero, Cristian Bassi, Lucilla Dâ€™Abundo, Antonio Frassoldati, Edi Simoni, Laura Astolfi, Enrico Maria Silini, Silvia Sabbioni, Massimo NegriniCell Signaling Technology) was used in combination with Clarity Western ECL Blotting Substrate (#170-5060; Bio-Rad) and digital images were acquired using a Chemidoc (Bio-Rad). Signals were quantified with

(18) Zebrafish mutants in vegfab can affect endothelial cell proliferation without altering ERK phosphorylation and are phenocopied by loss of PI3K signalingDevelopmental biologyJune 1, 2022Lange M, Ohnesorge N, Hoffmann D, Rocha SF, Benedito R, Siekmann AF.Cell Signaling), anti-p44/42 MAPK antibody (1:2000; #9102, Cell Signaling) and anti-αActin antibody (1:5000; A-5060, Sigma), Phospho-Akt (Ser473) (1:2000; #4060; Cell Signaling), Akt (1:1000; #9272; Cell Signaling).

(19) Effects of BPA, BPS, and BPF on Oxidative Stress and Antioxidant Enzyme Expression in Bovine Oocytes and SpermatozoaGenesJanuary 14, 2022Nguyen M, Sabry R, Davis OS, Favetta LA.Imaging System (Bio-Rad) after a 5 min incubation in Clarity Western ECL Blotting Substrate (Bio-Rad 170–5060). β-actin antibody (Cell Signalling Technology, 4967) at a 1:200 dilution overnight at 4 °C was used

(20) Transcriptome-Wide Analysis of RNA N(6)-Methyladenosine Modification in Adriamycin-Resistant Acute Myeloid Leukemia CellsFrontiers in geneticsJanuary 1, 2022Fang S, Peng B, Wen Y, Yang J, Wang H, Wang Z, Qian K, Wei Y, Jiao Y, Gao C, et al.h at room temperature. At last, the membrane was developed with Clarity Western ECL Substrate (170-5060, Bio-Rad, United States). RT-qPCR Total RNA was isolated by TRIzol Reagent. PrimeScript™ RT reagent

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RKIP (G38) Antibody

RKIP (G38) Antibody from Cell Signaling Technology

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Cell Signaling Technology

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RKIP (G38) Antibody from Cell Signaling Technology

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