BACE1 (D10E5) Rabbit mAb 5606S from Cell Signaling Technology

Product Specs
ItemBACE1 (D10E5) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5606S
Quantity100 µl
ApplicationsW, IP, IF-F, IF-IC
ReactivityH, M, R
TargetBACE
HostRabbit
NCBI Gene AliasesASP2, BACE, HSPC104
ClonalityMonoclonal
NCBI Full Gene Namebeta-secretase 1
Research AreaNeuroscience
IsotypeIgG
CloneD10E5
Molecular Weight70

Questions and Answers

Can this antibody be used to study BACE1 expression in Alzheimer's disease models?

Yes, the BACE1 (D10E5) Rabbit mAb has been utilized in studies investigating BACE1 expression in various models of Alzheimer's disease. For instance, it was shown to work in experiments involving organotypic brain slices and murine models, where BACE1 expression was linked to neuronal activity and amyloid-beta production ().

Is this antibody effective for immunofluorescence applications?

The BACE1 (D10E5) Rabbit mAb has been demonstrated to be effective for immunofluorescence applications, as indicated by its use in studies assessing BACE1 levels in human brain microvascular endothelial cells, where it played a role in understanding the effects of SARS-CoV-2 on BACE1 expression and subsequent cellular senescence ().

What species can this antibody detect?

This antibody is reactive with multiple species, including human, mouse, and rat, making it versatile for studies across different biological models.

Has this antibody been cited in research related to neuroinflammation?

Yes, the BACE1 (D10E5) Rabbit mAb has been cited in research examining neuroinflammation, particularly in studies that explore the role of BACE1 in Alzheimer's disease pathology and its involvement in neuroinflammatory processes ().

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Deletion of SPI1 in microglia exacerbates amyloid pathology by impairing microglial response in Alzheimer's disease modelsNeuronApril 1, 2026Byungwook Kim, Mason Douglas Tate, Hande Karahan, H.R. Sagara Wijeratne, Ahmad Daniel Sharify, Selena S. Wang, Justin R. Kim, Md Mamun Al-Amin, Kelly Hartigan, Sunwoo Chung, et al.precursor protein (βAPP; 1:500; #51–2700; Invitrogen), rabbit anti-β-secretase 1 (BACE1; 1:1000; CS-5606s; Cell Signaling Technology), mouse anti-Aβ (for detection βCTF, 1:500; IBL10323; IBL-AMERICA), and mouse

(2) Reshaping the gut microbiota: A novel oppinion of Eucommiae cortex polysaccharide alleviate learning and memory impairments in Alzheimer’s diseaseJournal of Advanced ResearchFebruary 1, 2026Yongkang Zhao, Wenxing Zhao, Xuejun Chai, Penghao Sun, Junlang Huang, Xinrui Guo, Lulu Zhang, Duoduo Ren, Chenju Yi, Xiaoyan Zhu, et al.included rabbit anti-β-Amyloid (8243, Cell Signaling Technology, Danvers, MA, USA), rabbit anti- BACE1 5606, Cell Signaling Technology, Danvers, MA, USA), rabbit anti- GSK-3α/β (5679, Cell Signaling Technology,

(3) Selective vulnerability of the aging cholinergic system to amyloid pathology revealed by induced APP overexpressionJournal of NeuroinflammationJanuary 7, 2026Kan Xie, Devon Ryan, Susanne Schröder, Lena Freund, Stefan Bonn, Yu Zhou, Dan Ehningeranti-human Aβ (#SIG-39300, clone 6E10, 1:2,000; Covance, Princeton, NJ, USA), rabbit monoclonal anti-BACE1 (#5606, clone D10E5, 1:2,000; Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-presenilin

(4) BACE1 regulates sleep–wake cycle through both enzymatic and non–enzymatic actionsEMBO ReportsNovember 20, 2025Hannah Heininger, Xiao Feng, Alp Altunkaya, Fang Zheng, Florian Stockinger, Benedikt Wefers, Stephan A Müller, Pieter Giesbertz, Sarah K Tschirner, Dorina Shqau, et al.Western Blot analysis. Immunoblot analysis Antibodies against BACE1 (D10E5, Cell Signaling Technology, #5606), APP (22C11, Merck Millipore, MAB348), soluble SEZ6 (polyclonal antibody, kindly provided by Dr. Jenny

(5) Microglia-specific regulation of lipid metabolism in Alzheimer's disease revealed by microglial depletion in 5xFAD MiceNat CommunOctober 15, 2025Ziying Xu, Sepideh Kiani Shabestari, Savannah Barannikov, Kevin F. Bieniek, Mathew Blurton-Jones, Juan Pablo Palavicini, Xianlin Hancatalog# mabg-mdect), anti-Tmem119 (1:100, Abcam, catalog# AB209064), anti-BACE1 (1:500, CST, catalog# 5606S), anti-P2Y12 (1:1000, CST, catalog# 69766S) at 4 °C overnight, washed three times, then incubated with

(6) APP SUMOylation prevents BACE1 cleavage of APP and increases BACE1 degradation to promote the nonamyloidogenic pathwayMolecular MedicineSeptember 29, 2025Yen-Chen Liu, Wei-Lun Hsu, Yun-Li Ma, Chung-Yao Yin, Kuang-Min Cheng, Eminy H. Y. Leefragment antibody (Catalog No. 802801, BioLegend, San Diego, CA), 3 μl of anti-BACE1 antibody (Catalog No. 5606, Cell Signaling, Danvers, MA) or 3 μl of anti-GFP antibody (Catalog No. 11814460001, Sigma-Aldrich)

(7) The novel role of Kallistatin in linking metabolic syndromes and cognitive memory deterioration by inducing amyloid-β plaques accumulation and tau protein hyperphosphorylationeLifeAugust 5, 2025Qi W, Long Y, Li Z, Zhao Z, Shi J, Xie W, Wang L, Tan Y, Zhou T, Liang M, et al.before (Li et al., 2019). The sections were incubated with Aβ (ab201060, Abcam, Cambridge, UK), BACE1 (#5606S, Cell Signaling Technology, Boston, USA), PPARγ (#2435, Cell Signaling Technology, Boston, USA), Notch1

(8) Hypercholesterolemia drives microglial dysfunction and weakens response to amyloid plaquesExperimental NeurologyAugust 1, 2025Sarah Kaye, Andrew Gold, Da Lin, Min Chen, Jiangjiang Zhu, Jie GaoAnti-Human Amyloid β (8243, Rabbit, 1:2000; 15126, Mouse, 1:500), Anti-ApoE (49285, 1:1000), Anti-BACE1 (5606, 1:1000), Anti-Lgals3 (89572, 1:500), and Anti-TREM2 (76765, 1:1000) from Cell Signaling Technology,

(9) Amyloid precursor protein dosage normalization rescues neurogenesis and Alzheimer′s Disease phenotypes associated with Down SyndromebioRxivAugust 1, 2025Deepika Patel, Karen Rakowiecki, Orly Lazarov,Thermo Fischer Scientific,MA5-15738 Rabbit anti-APP (WB 1:1000),Millipore Sigma,A8717 Rabbit anti-BACE1 (WB 1:1000),Cell signaling,5606S Mouse anti-AT8 (WB 1:1000),Thermo Fischer Scientific,MN1020 Mouse anti-Tau5 (WB 1:1000),Thermo Fischer Scientific

(10) Intranasal administration of Ganoderma lucidum-derived exosome-like nanovesicles ameliorates cognitive impairment by reducing inflammation in a mouse model of Alzheimer's diseaseFront PharmacolJuly 4, 2025Xue Mi, Xinglin Ruan, Renyi Lin, Shuxin Huang, Ping Cai, Xiaochun Chen, Jiangfeng Liao, Xiaoman Dai#9132; p-STAT3-Tyr705, 1:1,000, #9131; GFAP, 1:2,000, #3670; Caspase-8, 1:1,000, #9746; BACE, 1:1,000, #5606; NRF2, 1:1,000, #12721. Proteintech, Wuhan, China: ZBP1, 1:1,000, 22803-1-AP). The membranes were subsequently

(11) Neuroprotective Mechanisms of Porcine Brain Enzyme Hydrolysate in Memory Impairment: Multi-Target Strategy Against Amyloid-β-Induced NeurotoxicityInternational journal of molecular sciencesJune 24, 2025Yoon SM, Lee YW, Kim MJ, Shin JJ, Bae GW, Park S.GTX101648), choline acetyltransferase, (ChAT, Abcam; Cambridge, UK; ab181023), BACE (Cell Signaling; 5606S), p44/42 mitogen-activated protein kinase (MAPK, Erk1/2) (Cell Signaling, 9102S), phospho-p44/42 MAPK

(12) Edaravone-Dexborneol slows down pathological progression and cognitive decline via inhibiting S100A9 in APPswe/PS1dE9 miceAlzheimers Res TherJune 21, 2025Rui Mao, Shu Shu, Min Sun, Jiang Chen, Mengsha Hu, Lei Ye, Siyi Xu, Junqiu Jia, Wenxuan Shao, Xinyu Bao, et al.rabbit-anti MAP-2 (1:1000; Bioworld, bs3487), rabbit-anti BACE1 (1:1000; Cell Signaling Technology, 5606), rabbit-anti ADAM10 (1:1000; Abcam, ab1997), rabbit-anti S100A9 (1:1000; Proteintech, 26992-1-AP; cell

(13) Acute experimental colitis in 5xFAD Alzheimer''s disease mice leads to enhanced monocyte infiltration into the brain accompanied by reduced β-amyloid depositionAlzheimer's & dementia : the journal of the Alzheimer's AssociationJune 1, 2025Navalpur Shanmugam NK, Zamudio F, Vijaya Kumar DK, Barrett KA, VanDoren R, Chen M, Barr OM, Watson S, Lin CJ, Eimer WA, et al.C‐term APP, Millipore catalog #171610; 6E10, Biolegend catalog # 803012; BACE1, Cell Signaling catalog # 5606; sAPPβ, IBL, catalog # 18957; CD10, Santa Cruz Biotechnology, catalog # sc‐46656; IDE, abcam catalog

(14) BACE1 Inhibition Protects Against Type 2 Diabetes Mellitus by Restoring Insulin Receptor in MiceInternational Journal of Molecular SciencesMay 26, 2025Tingting Lin, Ting Liang, Yong Shen, Feng Gaoroom temperature for 1 h, and incubated overnight with primary antibodies: rabbit anti-BACE1 antibody (5606S, Cell signaling technology, Danvers, MA, USA), rabbit anti-Insulin receptor α antibody (74118S, Cell signaling technology

(15) ELK1 inhibition alleviates amyloid pathology and memory decline by promoting the SYVN1-mediated ubiquitination and degradation of PS1 in Alzheimer''s diseaseExperimental & molecular medicineMay 1, 2025Yi L, Li J, He Y, Wang J, Wang M, Guo S, Luo M, Wu B, Xu M, Tian Q, et al.ab3444) and anti-SYVN1 (1:500, ab170901) antibodies were purchased from Abcam. Anti-BACE1 (1:1000, #5606), anti-PEN2 (1:1000, #8598), anti-HA-Tag (1:1000, #3724) and anti-LC3 (1:1,000, #12741) antibodies were

(16) ApoE4 requires lipidation enhancement to resolve cellular lipid and protein abnormalities following NPC1 inhibitionScientific reportsApril 29, 2025Di Biase E, Connolly KJ, Crumpton I, Cooper O, Hallett PJ, Isacson O.anti-beta Amyloid (Thermo Fiscer Scientific #CT695, 1:500), anti-BACE1 (Cell Signaling Technology, #5606S, 1:1000), anti-p62 Cell Signaling Technology #5114, 1:1000) and anti-LC3 (Millipore #ABC232, 1:1000).

(17) The Role of Neprilysin and Insulin-Degrading Enzyme in the Etiology of Sporadic Alzheimer's DiseaseJ NeurosciApril 29, 2025Takahiro Morito, Shoko Hashimoto, Risa Takamura, Naoto Watamura, Naomasa Kakiya, Ryo Fujioka, Naomi Mihara, Misaki Sekiguchi, Kaori Watanabe-Iwata, Naoko Kamano, et al.(1:50, Synaptic Systems, 124-011), Lamp1 (1:200, Abcam, ab24170), and BACE1 (1:200, Cell Signaling, 5606S). After washing the sections three times with TNT for 5 min, secondary antibodies [Goat anti-Mouse IgG (H + L) Alexa Fluor 488

(18) Long-term systemic androgen deprivation partially modulates neuroinflammation in male AppNL-G-F/NL-G-F miceSci RepApril 27, 2025Kasumi Maekawa, Akira Sobue, Okiru Komine, Yuko Saito, Shigeo Murayama, Takaomi C. Saido, Takashi Saito, Koji YamanakaIBL, Japan), rat anti-CD68 (FA-11) (#MCA1957T, 1:1000, Bio-Rad, USA) and rabbit anti-BACE1 (D10E5) (#5606, 1:100, Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight. After washing with PBS, sections

(19) APP β-CTF triggers cell-autonomous synaptic toxicity independent of AβeLifeApril 23, 2025Luo M, Zhou J, Sun C, Chen W, Fu C, Si C, Zhang Y, Geng Y, Chen Y.used in this study: Y188 antibody (Abcam, ab32136, RRID:AB_ 2289606), D10E5 antibody (Cell Signaling, 5606, RRID:AB_1903900), Flag antibody (Sigma-Aldrich, F3165, RRID:AB_259529), β-Tubulin III antibody (Sigma,

(20) Integrated spatial multi-omics profiling of Fusobacterium nucleatum in breast cancer unveils its role in tumour microenvironment modulation and cancer progressionClin Transl MedMarch 11, 2025Feng Zhao, Rui An, Yilei Ma, Shaobo Yu, Yuzhen Gao, Yanzhong Wang, Haitao Yu, Xinyou Xie, Jun Zhangprimary antibodies targeting VEGFD (MCE, HY‐P82640, 1:500), PAK1 (MCE, HY‐P81618, 1:500), c‐Myc (CST, 5606S, 1:1000), phospho‐p38 MAPK (CST, 4511S, 1:1000), total p38 MAPK (CST, 9212S, 1:1000), phospho‐JNK1 (abcam,

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BACE (D10E5) Rabbit mAb

BACE1 (D10E5) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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BACE1 (D10E5) Rabbit mAb from Cell Signaling Technology

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