IRAK1 (D51G7) Rabbit mAb 4504S from Cell Signaling Technology

Product Specs
ItemIRAK1 (D51G7) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4504S
ClonalityMonoclonal
NCBI Full Gene Nameinterleukin 1 receptor associated kinase 1
ApplicationsWB, FCM, IP
ReactivityHu, Ms, Mk
Research AreaImmunology and Inflammation
IsotypeIgG
CloneD51G7
Molecular Weight78-105
NCBI Gene AliasesIRAK, pelle
HostRabbit
TargetIRAK1
Quantity100 µl

Questions and Answers

Can this antibody be used to study IRAK1 in the context of viral infections?

Yes, the IRAK1 (D51G7) Rabbit mAb has been utilized in studies examining its role in viral infections. For instance, it was shown to be involved in the regulation of type I interferon production during porcine reproductive and respiratory syndrome virus replication, highlighting its significance in immune response modulation during viral infections ().

Is this antibody effective for detecting IRAK1 in human samples?

The IRAK1 (D51G7) Rabbit mAb is reactive to human samples, as well as mouse and non-human primate samples. It has been cited in various studies that utilized it for Western blotting and immunoprecipitation in human cell lines, confirming its effectiveness in detecting endogenous levels of IRAK1 protein in human samples (, ).

What applications has this antibody been shown to work in?

This antibody has been demonstrated to be effective in multiple applications, including Western blotting, flow cytometry, and immunoprecipitation. It has been cited in numerous studies that employed these techniques to analyze IRAK1's role in various signaling pathways and immune responses (, ).

Are there any specific signaling pathways where IRAK1 plays a critical role that this antibody can help investigate?

Yes, IRAK1 is a key component in several signaling pathways, particularly those related to innate immunity and inflammation. The antibody has been used to study its involvement in the NF-κB signaling pathway, which is crucial for the regulation of inflammatory responses, as well as in the TLR signaling pathways that activate immune responses (, ).

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A druggable redox switch on SHP1 controls macrophage inflammationNature Chemical BiologyMarch 12, 2026Mei Ying Ng, Meredith N. Nix, Guangyan Du, Ivan Davidek, Nils Burger, Sanghee Shin, Sean Toenjes, Haruna Takeda, Megan Cheah Xin Cheah Xin Yan, Bingsen Zhang, et al.(Thermo Fisher Scientific, iBlot 2 Gel Transfer Device, #IB21001), and immunoblotted with anti-IRAK1 (Cell Signaling, #4504, rabbit mAb; 1:1000 dilution), anti-phospho-IκBα (Ser32/36) (Cell Signaling, #9246; mouse mAb; 1:1000

(2) SLC12A8 Drives Immune Evasion and Metastasis in Luminal B Breast Cancer by Inducing CD8 + T‐Cell Exhaustion via the TLR Signaling PathwayCancer MedMarch 8, 2026Zhiyong Liu, Ran Chenanti‐TLR2 (Cell Signaling Technology, 12,276, 1:1000), anti‐MyD88 (CST, 4283, 1:1000), anti‐IRAK1 (CST, 4504, 1:1000), anti‐TAK1 (CST, 4505, 1:1000), anti‐PD‐1 (CST, 86163, 1:1000), and internal reference anti‐β‐actin

(3) PSP-0119: Targeted IRAK4 Degradation as a Novel Therapeutic Strategy for FLT3-Mutant AMLbioRxivOctober 7, 2025Negar Khazan, Cameron WA Snyder, Chandhana Ravi, Elizabeth Lamere, Niloy A. Singh, Manoj K. Khera, Jane Liesveld, Srinivasan Ekambaram, Nikolay V. Dokholyan, Myla Strawderman, et al.(Cell Signaling Tech, cat. no:4363, dilution: 1:1000), rabbit IRAK1 antibody (Cell Signaling Tech, cat. no:4504, dilution: 1:1000). Membranes were striped with OneMinute stripping buffer (GM Biosciences, cat no:GM6001)

(4) Human inborn errors of long-chain fatty acid oxidation show impaired inflammatory responses to TLR4-ligand LPSFASEB bioAdvancesSeptember 1, 2024Mosegaard S, Twayana KS, Denis SW, Kroon J, Schomakers BV, van Weeghel M, Houtkooper RH, Olsen RKJ, Holm CK.mentioned). The one used for this study were: anti‐TLR4 (sc‐293,072, Santa Cruz Biotechnology), anti‐IRAK1 (#4504, CST), anti‐P‐IRAK1(# PA5‐38633, Thermo Fischer Scientific), anti‐IκBα (#4814, CST), anti‐Phospho‐IκBα

(5) Effects of liver X receptor in O3-induced airway inflammation and remodeling in miceJournal of Thoracic DiseaseAugust 31, 2024Fenfang Yu, Jiyong Ma, Ke Xu, Yu Tang, Bining Wu, Wei Gu, Ying Shiantibodies [β-actin (CST, 4970S), MyD88 (CST, 4283S), interleukin-1 receptor-associated kinase (IRAK) (CST, 4504S)] were diluted in tris buffered saline tween (TBST) buffer and incubated with the membrane overnight

(6) An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndromeDisease models & mechanismsJuly 1, 2024Maino E, Scott O, Rizvi SZ, Chan WS, Visuvanathan S, Zablah YB, Li H, Sengar AS, Salter MW, Jia Z, et al.anti-MECP2 (Abcam, ab253197, 1:3000), rabbit monoclonal D51G7 anti-IRAK1 (Cell Signaling Technology, #4504, 1:1000), rabbit monoclonal anti-GAPDH (Santa Cruz Biotechnology, sc-47724, 1:10,000) – overnight at

(7) Selective targeting of IRAK1 attenuates low molecular weight hyaluronic acid-induced stemness and non-canonical STAT3 activation in epithelial ovarian cancerCell death & diseaseMay 25, 2024Standing D, Dandawate P, Gunewardena S, Covarrubias-Zambrano O, Roby KF, Khabele D, Jewell A, Tawfik O, Bossmann SH, Godwin AK, et al.(dilution 1:2000) and developed by ECL detection reagents (Amersham-Pharmacia, Piscataway, NJ). IRAK1 (4504S), pSTAT3 (9145S), STAT3 (9139S), MYC (9402S), KLF4 (12173S), p-p65 (13346S), p65 (8242S), p-p38 (9216S),

(8) Response to Bruton’s tyrosine kinase inhibitors in aggressive lymphomas linked to chronic selective autophagyCancer CellFebruary 1, 2024James D. Phelan, Sebastian Scheich, Jaewoo Choi, George W. Wright, Björn Häupl, Ryan M. Young, Sara A. Rieke, Martine Pape, Yanlong Ji, Henning Urlaub, et al.AB_10547882 BCL10,Cell Signaling Technology,Cat.# 4237S; RRID: AB_2228005 IRAK1,Cell Signaling Technology,Cat.# 4504S; RRID: AB_1904032 MALT1,Cell Signaling Technology,Cat.# 2494S; RRID: AB_2139139 Ubiquitin (P4D1),Santa Cruz

(9) Development of the nonreceptor tyrosine kinase FER-targeting PROTACs as a potential strategy for antagonizing ovarian cancer cell motility and invasivenessThe Journal of biological chemistryJune 1, 2023Zhang Y, Xiong X, Sun R, Zhu X, Wang C, Jiang B, Yang X, Li D, Fan G.(Cell Signaling Technology; catalog no.: 79832), CHK2 (Abclonal; A2145), IRAK1 (Cell Signaling Technology; catalog no.: 4504), CRBN (Abclonal; A4722), and GAK (Proteintech; catalog no.: 12147-1-AP). The secondary antibodies were

(10) MiR-146a-5p delivered by hucMSC extracellular vesicles modulates the inflammatory response to sulfur mustard-induced acute lung injuryStem cell research & therapyMay 30, 2023Pei Z, Cen J, Zhang X, Gong C, Sun M, Meng W, Mao G, Wan J, Hu B, He X, et al.antibodies against TLR4 (1:200, sc-293072, Santa Cruz, USA), TRAF6 (1:1000, 67591, CST, USA), IRAK1 (1:1000, 4504, CST, USA), NF-κB (1:1000, 4764, CST, USA), phospho-NK-κB (1:1000, 3033, CST, USA), β-Actin (1:1000,

(11) Identification of ester-linked ubiquitylation sites during TLR7 signalling increases the number of inter-ubiquitin linkages from 8 to 12Biochemical JournalDecember 9, 2022Elisha H. McCrory, Vyacheslav Akimov, Philip Cohen, Blagoy BlagoevNew England Biolabs (#P0753S). Antibodies The antibodies recognising IRAK1 (Cell Signalling Technology #4504S) and IRAK2 (Abcam #ab62419) were purchased from the sources indicated in parentheses. The antibody recognising

(12) PHB2 affects the virulence of Vip3Aa to Sf9 cells through internalization and mitochondrial stabilityVirulenceDecember 1, 2022An B, Zhang Y, Li X, Hou X, Yan B, Cai J.Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-GST (#AE067), rabbit anti-actin (#AC004), rabbit anti-PHB2 (#4504), Alexa Fluor 594-conjugated goat anti-rabbit IgG (#AS039), Alexa Fluor 594-conjugated goat anti-mouse

(13) Co-ordinated control of the ADP-heptose/ALPK1 signalling network by the E3 ligases TRAF6, TRAF2/c-IAP1 and LUBACThe Biochemical journalOctober 28, 2022Snelling T, Shpiro N, Gourlay R, Lamoliatte F, Cohen P.(#2368), GAPDH (#2118), human c-IAP1 (#7065), human c-IAP2 (#3130), IκBα (#4812), IKKβ (#8943), IRAK1(#4504), NIK (#4994), Pellino 1 (#31474), human TIFA (#61358), TRAF2 (#4712) and human TRAF6 (#8028). An Antibody

(14) TAK1 protein kinase activity is required for TLR signalling and cytokine production in myeloid cellsThe Biochemical journalSeptember 16, 2022Rodrigues M, Petrova T, Tibbs B, Arthur JSC, Cohen P.and those recognising all forms of TAK1 (#4505), IκBα (#(L35A5), TAB2 (#3745), TAB3 (#14241), IRAK1 (#4504), IRAK2 (#4367), IRAK3 (#4369), IRAK4 (#4363), MyD88 (#4823), p38α MAP kinase (#9212) and GAPDH (#2118)

(15) The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathwaysCell reportsAugust 16, 2022Pereira M, Durso DF, Bryant CE, Kurt-Jones EA, Silverman N, Golenbock DT, Gazzinelli RT.al., 2012). The following antibodies and dilutions were used for immunoblots: IRAK1 (Cell Signaling, 4504S, 1:1000), IRAK2 (Abcam, ab62419, 1:500), TRAF6 (Abcam, ab33915, 1 in 500), TRAF3 (Abcam, ab239357, 1:400),

(16) Regulatory role of miR-146a in corneal epithelial wound healing via its inflammatory targets in human diabetic corneaThe ocular surfaceJuly 1, 2022Poe AJ, Shah R, Khare D, Kulkarni M, Phan H, Ghiam S, Punj V, Ljubimov AV, Saghizadeh M.Antigen,Antibody,Source,MW (kDa),Dilution TRAF6,Rabbit mAb 8028S,Cell Signaling,60,1:500 IRAK1,Rabbit mAb 4504S,Cell Signaling,78,1:500 NF-κB,Rabbit mAb 8242S,Cell Signaling,65,1:1000 p-NF-κB,Rabbit mAb 3033S,Cell Signaling

(17) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-PolarizationCellsJanuary 11, 2022Fan CS, Chen CC, Chen LL, Chua KV, Hung HC, Hsu JT, Huang TS.#ab2068, Abcam), mouse anti-CD91 (1:80, cat. #550495, BD Biosciences) plus rabbit anti-IRAK1 (1:80, cat. #4504, Cell Signaling, Danvers, MA, USA), rabbit anti-MyD88 (1:100, cat. #ab2068, Abcam) plus mouse anti-IRAK1

(18) KIZ/GM114 Balances the NF-ĸB Signaling by Antagonizing the Association of TRAF2/6 With Their Upstream AdaptorsFrontiers in cell and developmental biologyJanuary 1, 2022Sun J, Yang Q, Liu E, Chen D, Sun Q.anti-p-TAK1 (T184/187,4508), anti-TAK1 (5206), anti-p-IKKα/β (S176/180, 2697), anti-IKKβ (8943), anti-IRAK1 (4504), anti-IκBα (9242) and mouse anti-p-IκBα (Ser32/36, 9246) antibodies were purchased from Cell Signaling Technology

(19) Comprehensive Pan-Cancer Analysis of IRAK Family Genes Identifies IRAK1 as a Novel Oncogene in Low-Grade GliomaJournal of oncologyJanuary 1, 2022Li J, Sun Y, Ma Y, Zhao X, Sun X, Wang Y, Zhang X.(TBST). Then, all membranes were incubated overnight at 4°C with the primary antibodies: anti-IRAK1 (#4504; Cell Signaling Technology, MA, USA; 1 : 1000), anti-E-cadherin (20874-1-AP, Proteintech, 1 : 5000),

(20) Role of extracellular microRNA-146a-5p in host innate immunity and bacterial sepsisiScienceDecember 17, 2021Wang S, Yang Y, Suen A, Zhu J, Williams B, Hu J, Chen F, Kozar R, Shen S, Li Z, et al.,Cell Signaling Technology,Cat#5632; RRID:AB_10692895 IRAK1 (D51G7) Rabbit mAb,Cell Signaling Technology,Cat#4504; RRID:AB_1904032 GAPDH (14C10) Rabbit mAb,Cell Signaling Technology,Cat#2118; RRID:AB_561053 IκBα

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8/26/2022Great Anti-IRAK1 Antibody Liyong Z.BT20 cells were treated with 30 ng/ml TNFalpha for 30 minutes and IRAK1 was examined by Western blot.

IRAK1 (D51G7) Rabbit mAb

IRAK1 (D51G7) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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IRAK1 (D51G7) Rabbit mAb from Cell Signaling Technology

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