Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb 4199S from Cell Signaling Technology

Product Specs
ItemCleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number4199S
Quantity100 µl
ApplicationsW, IP
ReactivityH
TargetCasp1 (p20) cleaved
HostRabbit
NCBI Gene AliasesICE, IL1BC, P45
ClonalityMonoclonal
NCBI Full Gene Namecaspase 1
Research AreaApoptosis
IsotypeIgG
CloneD57A2
Molecular Weight20, 22

Questions and Answers

Can this antibody be used to study the NLRP3 inflammasome in human cells?

Yes, the Cleaved Caspase-1 (Asp297) antibody has been utilized in studies investigating the NLRP3 inflammasome. For instance, it was employed to assess caspase-1 cleavage in THP-1 cells, which are often used as a model for studying inflammasome activation ().

What applications has this antibody been shown to work in?

The Cleaved Caspase-1 antibody is suitable for Western blotting and immunoprecipitation. It has been cited in various studies for detecting cleaved caspase-1 levels, which is crucial for understanding apoptosis and inflammatory responses (, ).

Is this antibody effective for detecting cleaved caspase-1 in different experimental setups?

Yes, the antibody has been shown to effectively detect cleaved caspase-1 in various experimental contexts, including in vivo and in vitro studies. For example, it was used to evaluate the effects of treatments on caspase-1 activation in models of cerebral ischemia and inflammation (, ).

What specific human protein does this antibody target, and why is it important?

This antibody targets the p20 subunit of human caspase-1, specifically upon cleavage at Asp297. This cleavage is a critical step in the activation of caspase-1, which plays a key role in the inflammatory response and pyroptosis, making it significant for studies related to apoptosis and inflammation ().

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signalingNeurobiology of StressJune 1, 2026Rui Xu, Chi Zhang, Ke Chen, Yue Shi, Chun-Rong Liang, Chun-Yan Long, Hui-Yun Li, Cheng-Kang He, Ling LiAbcam), KEAP1 (8047, Cell Signaling Technology), NLRP3 (AG-20B-0014, AdipoGen), cleaved caspase-1 (4199, Cell Signaling Technology), cleaved IL-1β (83186, Cell Signaling Technology), and β-actin (A1978, Sigma-Aldrich).

(2) Extracellular caspase-1: a critical inducer and a therapeutic target of lung injury in gut ischemia-reperfusionFront ImmunolApril 15, 2026Kouhei Ishikawa, Gaifeng Ma, Alok Jha, Atsushi Murao, Ping Wang, Monowar Azizaccording to the manufacturers’ instructions: anti-cleaved caspase-1 (Asp297) (p20 subunit; Cat. No. 4199S, 1:1000) and anti-cleaved caspase-1 (Asp296) (p20 subunit; Cat. No. 89332S, 1:1000) (all from Cell Signaling Technology

(3) Specific inhibition of NLRP3 inflammasome by a Smurf1 inhibitor in vitro and in vivoOpen Med (Wars)April 9, 2026Lifeng Meng, Hongmei Xuan, Ping Zhou, Weifeng Xu, Wei Bian, Yunxiao Lin, Ying GuanAG-20B-0042) and anti-NLRP3 (1:2000, AG-20B-0014) were purchased from Adipogen. Anti-human caspase-1 (1:2000, 4199S) was purchased from Cell Signaling Technology. Anti-ASC (1:1000, sc-22514-R) were purchased from Santa Cruz

(4) Hematopoietic cell kinase regulates microglial/macrophage activation to drive neuroinflammation after traumatic brain injuryCell Communication and SignalingApril 9, 2026Lei Li, Taoyuan Lu, Weiwei Gao, Fanjian Li, Xin Guo, Siyu Guan, Dilmurat Gheyret, Jinchao Wang, Tuo Li, Yiyao Cao, et al.NLRP3 (Cat# 19771-1-AP; Proteintech), cst ASC (CAT# ab309497; Abcam), cleaved (CL)-Caspase-1 (Cat# 4199; CST), IL-1β (Cat# ab283822; Abcam), and β-actin (Cat# 4967; CST). After washing, membranes were incubated

(5) Statins targeting mevalonate-geranylgeranyl diphosphate pathway inhibit proliferation in NK/T lymphoma by promoting pyroptosisTransl OncolApril 2, 2026Yingjun Wang, Mei Mei, Jieting Wang, Lai Wei, Mengqian Zhang, Mingzhi Zhangprimary antibodies against pro-caspase-1 (1:1000, ab179515, Abcam, England), cleaved caspase-1 (1:1000, 4199, CST, USA), GSDMD (1:2000, 20770-1-AP, Proteintech, Wuhan, China), cleaved N-terminal GSDMD (1:1000,

(6) Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial CellsJ Inflamm ResMarch 7, 2026Fan He, Chunzi Liu, Yun Yu, Qiaofang Wang, Lin Li, Xiaoyun Chao, Liping Chen, Cong Yudescribed.23 The following primary antibodies were used: anti-cleaved caspase-1 (1:1000; Cat. No. #4199; Cell Signaling Technology, MA, USA), anti-MT2 (1:1000; Cat. No. ab203346; Abcam, Cambridge, UK), anti-ETS1

(7) Melatonin Suppresses NLRP3 Inflammasome Activation via SIRT1-Mediated ETS1 Deacetylation to Attenuate LPS-Induced Pyroptosis in Alveolar Epithelial CellsJ Inflamm ResMarch 7, 2026Fan He, Chunzi Liu, Yun Yu, Qiaofang Wang, Lin Li, Xiaoyun Chao, Liping Chen, Cong Yudescribed.23 The following primary antibodies were used: anti-cleaved caspase-1 (1:1000; Cat. No. #4199; Cell Signaling Technology, MA, USA), anti-MT2 (1:1000; Cat. No. ab203346; Abcam, Cambridge, UK), anti-ETS1

(8) STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degenerationCell Death & DiseaseFebruary 27, 2026Chao Huang, Vishnu Suresh Babu, Sridhar Bammidi, Jakob N. Arnold, Martin Ebeling, Gabriella Widmer, Pamela Strassburger, Mirjana Lazendic, Sabine Grüner, Janis Koester, et al.Cell Signaling Technology, Cat. #4904), anti-cleaved caspase 1 (1:1000, Cell Signaling Technology, Cat. #4199), anti-H3 (1:2000, Cell Signaling Technology, Cat. #4499, Cell Signaling Technology), anti-β-Actin (1:2000,

(9) H3K18 lactylation-mediated SPHK1-SIRT1 feedback loop accelerates pyroptosis of tubular epithelial cells in sepsis-associated acute kidney injuryTheranosticsFebruary 18, 2026Yan Huang, Eryang Zhao, Guangyu Zhao, Wenfeng Zhuo, Yingsong Zhao, Hongda Wang, Guozheng Lv, Rong Hu, Zhu Zeng, Shengbo Han, et al.H3K18la (PTM-1427RM, PTM Bio), Cleaved Caspase-1 (89332, CST), P300 (ab275378, Abcam), Cleaved Caspase-1 (4199, CST), NLRP3 (P60622R3, Abmart), SIRT1 (13161-1-AP, Proteintech), NLRC4 (PB0658, Boster), PGC-1α (66369-1-Ig,

(10) Investigating the impact and mechanism of Licochalcone B derivative CTG12 on NLRP3 inflammasomeCell Communication and SignalingFebruary 18, 2026Shuyi Bian, Zhi-E Fang, Lichen Wang, Jiayi Wang, Ming Dong, Nan Yang, Jiabo Wang, Ling Li, Guang XuPromega (USA). Anti-human IL-1β (p17, 12242S), anti-Phospho-IRF3 (Ser396) (4947), caspase-1 antibody (4199S) and anti-ASC antibody (sc-22, 514-R) were obtained from Cell Signaling Technology (Germany). Active

(11) LIN28B Aggravates the Inflammation in Sepsis-Induced Lung Injury by Stabilizing GATA6 mRNA and Upregulating TRAF6Pulm CircFebruary 10, 2026Lingjia Chen, Jiangchuan Chen, Zeqiang Wang, Xin Jiang, Jun Cao, Huan Yang, Xiaotian Wen, Jianmin Li(ab182858, 1:2000, Abcam), anti‐NLRP3 antibody (ab263899, 1:1000, Abcam), anti‐cleaved caspase‐1 antibody (#4199, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti‐GSDMD‐N antibody (ab215203, 1:1000, Abcam),

(12) TFAP2A transcriptionally regulates B3GALT2 to affect gouty arthritis progression through pyroptosis: a study based on machine learning and multi-omics integration analysisJournal of Orthopaedic Surgery and ResearchFebruary 8, 2026Hui-Li Han, Zeng-Yu Cheng, Qing-Liang Meng, Xu-Zhao DuScientific,MBS766996 anti-NLRP3,1:1000,Abcam,ab263899 anti-ASC,1:1000,Abcam,ab283684 anti-GSDMD-N,1:1000,Abcam,ab215203 anti-Caspase-1 p20,1:1000,Cell Signaling Technology,4199 anti-TFAP2A,1:5000,Abcam,ab108311 anti-β-actin,1:5000,Abcam,ab8226 Goat Anti-Rabbit IgG H&L (HRP),"1:20,000",Abcam,ab6721 Rabbit Anti-Mouse IgG H&L (HRP

(13) HIF-1α/BNIP3-mediated mitophagy mitigates cerebral ischemia/reperfusion injury in rats by suppressing NLRP3 inflammasome activationEur J Med ResJanuary 31, 2026Qifan Huo, Yuming Zhang, Jing Zhao, Ning Bai, Jun Wang(ECL) reagent and quantified using ImageJ software. The primary antibody for cleaved caspase-1(1:1000, #4199) was obtained from Cell Signaling Technology, and others were sourced from Abcam, including HIF-1α (1:1000,

(14) Targeting the GPX4–FUNDC1 Interaction with Magnesium Lithospermate B Attenuates Sepsis‐Associated Lung InjuryAdvanced ScienceJanuary 30, 2026Zhixi Li, Chang Liu, Zhaoxue Ma, Dongyou Zheng, Renkai Wang, Yongjing Yu, Guangmin Chen, Chenglong Li, Yue Bu, Hang Cao, et al.USA), Cleaved Caspase‐3 (1:1000, 9505, Cell Signaling Technology, MA, USA), Cleaved Caspase‐1 (1:1000, 4199, Cell Signaling Technology, MA, USA) and Cleaved Gasdermin D (1:1000, 36 425, Cell Signaling Technology,

(15) The NLRP3 inhibitor, MCC950, attenuates environmental pollutant PM2.5-induced acute lung injury by inhibiting alveolar macrophage pyroptosisCurrent Research in ToxicologyJanuary 22, 2026Zhuoxiao Han, Ying Han, Ranran Li, Kexin Fan, Xiao Zhang, Tengfei Sun, Yue Li, Hua QiaoCell Signaling Technology‌ (CST), USA,1:1000), Pro-Caspase1 (cat. no. 2225, CST, 1:1000), Caspase1 (cat. no. 4199, CST, 1:1000), IL1β (cat. no. 12242, CST, 1:1000), IL18 (cat. no. 67775, CST, 1:1000), GSDMD-NT (cat.

(16) Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell LinesInternational Journal of Molecular SciencesJanuary 7, 2026Santhalakshmi Ranganathan, Tolulope Ojo, Alagu Subramanian, Jenna Tobin, Alexander Kornienko, Angela Boari, Antonio Evidente, Mary Lauren Benton, Daniel Romo, Joseph H. TaubeUSA), pMLKL (ab187091, Abcam, Cambridge, UK), Caspase3 (9662S, Cell Signaling Technology), Caspase1 (4199, Cell Signaling Technology), Gasdermin D (ab215203, Abcam), Actin (612657, BD Bioscience), HRP-conjugated

(17) Palmatine Attenuates Lipopolysaccharide-Induced Acute Lung Injury Via Suppression of NLRP3 Inflammasome Activation, Pyroptosis, and Metabolic RemodelingInflammationDecember 20, 2025Pu Ning, Jing Wu, Qiuyue Long, Yaolin Zheng, Jialing Gan, Xiaoyi Hu, Shixu Song, Xiaomin Li, Mingzheng Jiang, Hongli Ye, et al.Arg-1 (16001-1-AP), and GAPDH (81640-5-RR) were from Proteintech (Wuhan, China); cleaved caspase-1 p20 (4199T) from Cell Signaling Technology (Danvers, MA, USA); cleaved N-terminal GSDMD (ab215203) and caspase-11

(18) Identification of a reactive astrocyte subpopulation during HIV-associated pain pathogenesis in mouse modelsNature CommunicationsDecember 12, 2025Junying Zheng, Michael Spurgat, Mark Maurelli, Zachary Watson, Sakib Hossain, Adriana Dibua, Subo Yuan, Xin Liu, Benjamin B. Gelman, Shao-Jun TangCell Signaling Technology, cat. no. 12242), anti-cleaved caspase-1 (1:1000, Cell Signaling Technology, cat. no. 4199), anti-wnt5a (1:1000, Abcam, cat. no. ab72583), anti-Iba1 (1:1000, Wako, cat. no. AB_839506), and anti-actin

(19) KIF1B Regulates NLRP3-Mediated Pyroptosis in Asthma ProgressionJ Cell Mol MedDecember 12, 2025Junchao Wang, Yuan Gao, Jing Li, Chiqiu Jianganti‐KIF1B (1:1000, ab234869, Abcam), anti‐NLRP3 (1:1000, ab263899, Abcam), anti‐cleaved caspase‐1 (1:1000, #4199, Cell Signaling Technologies) and anti‐GSDMD‐N (1:1000, #36425, Cell Signaling Technologies) overnight

(20) The sphingolipid metabolite sphingosine protects against hypertension by targeting metabolic-inflammatory crosstalk via the NLRP3 inflammasomeInt J Cardiol Cardiovasc Risk PrevDecember 11, 2025Wenjun Li, Dan Zhou, Yanmei Ji, Meirong Yang, Yunhong Yang, Mengyao Dao, Xianyu He, Xingfang Jinantibody incubation, were performed as described in s ection 1.3.5. Except Cleaved Caspase-1 (CST, Cat# 4199), Cleaved IL-1β (CST, Cat# 83186), GSDMD(N-Terminal) (Abcam, Cat#ab215203), NLRP3 (Proteintech, Cat#27458-1-AP),

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Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb

Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb from Cell Signaling Technology

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