IL-10 R alpha Antibody MAB274-100 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-10 R alpha Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberMAB274-100
PurityProtein A or G purified from hybridoma culture supernatant
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ReactivityHuman (Hu)
ClonalityMonoclonal
NCBI Gene AliasesCD210a, HIL-10R, IL-10R1, IL10R, CDW210A, CD210
NCBI Full Gene Nameinterleukin 10 receptor subunit alpha
ApplicationsFCM, BL, FA, Neut
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10 R alpha, His22-Asn235, Accession # Q13651.1
HostMouse
Clone37607
IsotypeIgG1
TargetIL-10 R alpha
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug, 500 ug)

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL-10 R alpha.

Sizes Available: 100 ug (also 25 ug, 500 ug)

Add to Compare List

Questions and Answers

Can this antibody be used for flow cytometry applications?

Yes, the IL-10 R alpha antibody has been shown to work in flow cytometry applications, allowing for the detection of human IL-10 R alpha on cell surfaces.

Is this antibody specific for human IL-10 R alpha, and does it cross-react with mouse IL-10 R alpha?

The IL-10 R alpha antibody specifically detects human IL-10 R alpha and does not cross-react with recombinant mouse IL-10 R alpha, making it suitable for studies focused on human samples.

What is the immunogen used for this antibody, and how does it relate to its specificity?

The immunogen for the IL-10 R alpha antibody is derived from the S. frugiperda insect ovarian cell line Sf 21 and consists of recombinant human IL-10 R alpha. This specific immunogen contributes to the antibody's ability to selectively bind to human IL-10 R alpha.

How should this antibody be stored after reconstitution to maintain its stability?

After reconstitution, the IL-10 R alpha antibody should be stored under sterile conditions at -20 to -70 °C for up to six months to maintain its stability, and it should be kept away from repeated freeze-thaw cycles.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Transcription Factor c-Maf Promotes Immunoregulation of Programmed Cell Death 1-Expressed CD8(+) T Cells in Multiple SclerosisNeurology(R) neuroimmunology & neuroinflammationJuly 1, 2022Koto S, Chihara N, Akatani R, Nakano H, Hara A, Sekiguchi K, Matsumoto R, Toda T.

(2) Ex vivo-expanded human CD19(+)TIM-1(+) regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axisNature communicationsJune 3, 2022Shankar S, Stolp J, Juvet SC, Beckett J, Macklin PS, Issa F, Hester J, Wood KJ.

(3) Identification of a CD4(+) T cell line with Treg-like activityHuman immunologyApril 1, 2022Ho TH, Pfeffer K, Weiss GJ, Ruiz Y, Lake DF.

(4) IL-10 mRNA Engineered MSCs Demonstrate Enhanced Anti-Inflammation in an Acute GvHD ModelCellsNovember 10, 2021Zhang C, Delawary M, Huang P, Korchak JA, Suda K, Zubair AC.

(5) Targeting of interleukin-10 receptor by a potential human interleukin-10 peptide efficiently blocks interleukin-10 pathway-dependent cell proliferationCi Ji Yi Xue Za ZhiJuly 1, 2020Chang CC, Liu CD, Pan SF, Huang WH, Peng CW, Hsu HJ.

(6) Microenvironmental IL1β promotes breast cancer metastatic colonisation in the bone via activation of Wnt signallingNature communicationsNovember 1, 2019Eyre R, Alférez DG, Santiago-Gómez A, Spence K, McConnell JC, Hart C, Simões BM, Lefley D, Tulotta C, Storer J, et al.

(7) Immune Checkpoint Blockade Restores HIV-Specific CD4 T Cell Help for NK CellsJournal of immunology (Baltimore, Md. : 1950)August 1, 2018Porichis F, Hart MG, Massa A, Everett HL, Morou A, Richard J, Brassard N, Veillette M, Hassan M, Ly NL, et al.

(8) Inhibition of IL-10 and TGF-β receptors on dendritic cells enhances activation of effector T-cells to kill cholangiocarcinoma cellsHuman vaccines & immunotherapeuticsJune 3, 2018Thepmalee C, Panya A, Junking M, Chieochansin T, Yenchitsomanus PT.

(9) IL-10 Receptor Signaling Is Essential for TR1 Cell Function In VivoJournal of immunology (Baltimore, Md. : 1950)February 1, 2017Brockmann L, Gagliani N, Steglich B, Giannou AD, Kempski J, Pelczar P, Geffken M, Mfarrej B, Huber F, Herkel J, et al.

(10) Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophagesScientific reportsAugust 25, 2016Huynh L, Kusnadi A, Park SH, Murata K, Park-Min KH, Ivashkiv LB.

(11) Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanismsGenes and immunityJanuary 1, 2015von Haehling S, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, et al.

(12) Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablastsEuropean journal of immunologyJune 1, 2014Heine G, Drozdenko G, Grün JR, Chang HD, Radbruch A, Worm M.

(13) IFNγ/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed childrenPLoS pathogensJanuary 1, 2014Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo MK, Arinaitwe E, Briggs J, et al.

(14) TNF-α blockade induces IL-10 expression in human CD4+ T cellsNature communicationsJanuary 1, 2014Evans HG, Roostalu U, Walter GJ, Gullick NJ, Frederiksen KS, Roberts CA, Sumner J, Baeten DL, Gerwien JG, Cope AP, et al.

(15) Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1European journal of immunologyDecember 1, 2008Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C.

(16) Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infectionJournal of virologyApril 1, 2008Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B.

(17) Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor functionBloodJanuary 15, 2005Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB.

(18) Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionalityJournal of virologyAugust 1, 2004Chang WL, Baumgarth N, Yu D, Barry PA.

(19) In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR culturesBloodJuly 15, 2004Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB.

Be the First to Write a Review!