WB of rat brain lysate showing detection of ~94 kDa GRP94 using Anti-GRP94. Load: 20 ug RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rabbit Anti-GRP94 polyclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~ 94-100 kDa. Other band (s): ~50, ~75 kDa.