human cell line A431; Primary antibody dilution and incubation time: 1: 600, 4 degree overnight.Secondary antibody used and dilution and incubation time: 1: 3000, RT 2 hours

Lanes: 1. Untransfected MIA-PACA2 cell lysate; 2. Untransfected MIA-PACA2 cell lysate; 3. Untransfected MIA-PACA2 cell lysate; 4. hSEMA4F transfected MIA-PACA2 cell lysate; 5. Untransfected MIA-PACA2 cell lysate; 6. hSEMA4F transfected MIA-PACA2 cell lysa

Lanes: 1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNA; Primary Antibody Dilution: 1: 600; Secondary Antibody: Donkey Anti-rabbit HRP; Secondary Antibody Dilution: 1: 3000; Gene Name: SEMA4F; Submitted by: Dr. Tianliang Sun, Max Pla