Fig 2: Mical1 fl/fl , Tyr::Cre mutant mice had no differences in rotarod or vertical pole tests. (A) Mice were on a rod rotating at 40 rpm and the time for them to fall off or to allow themselves to passively rotate was recorded. Averages of three independent trials performed each day are reported for each mouse. Graphs indicate means ± standard deviation. Statistical test with one‐way ANOVA and post‐hoc Tukey's multiple comparisons test. p values <0.05 indicated in bold typeface, p values ≥0.05 indicated in italic typeface. N = 9 mice per genotype. (B) The time taken for mice to turn downwards after having been placed in the centre of a 60 cm pole that was tilted 90°. (C) The time taken for mice to descend from the centre of a 60 cm pole that was tilted 90°. Results presented are the averages from three independent trials for each mouse. Graphs indicate means ± standard deviation. Statistical tests with Student's t‐test. p values ≥0.05 indicated in italic typeface. N = 9 mice per genotype. KO, knockout; WT, wild‐type.

Fig 3: No effect in Mical1 fl/fl , Tyr::Cre mutant mice on their movement across balance beams. (A) Time taken for mice to cross 90 cm round beams of varying widths (24, 12 and 6 mm). (B) The number of hind foot slips during the crossing of the balance beams. Results presented are the averages from two independent trials for each mouse. Graphs indicate means ± standard deviation. Statistical test with one‐way ANOVA and post‐hoc Tukey's multiple comparisons test. p values ≥0.05 indicated in italic typeface. N = 9 mice per genotype. KO, knockout; WT, wild‐type.

Fig 4: Mical1 fl/fl , Tyr::Cre mutant mice had reduced run speed and stride frequency in male mice. (A) Overall average run speeds were determined by analyzing high‐speed videos of male mice running on a treadmill for 20 s. Overall running speed (mm/s) was obtained by dividing the total distance traveled by the center of the animal by the time it took to travel that distance. Results presented are for each mouse. (B) Instantaneous running speed (mm/s) of a stride is the ratio of the stride length to the stride time. Results presented are for each mouse. (C) Stride frequency is the ratio of the number of strides to the sum of the stride times of these strides, which yields the number of strides per second (Hz) for a given foot. Results presented are for all 4 ft. Graphs indicate means ± standard deviation. Statistical tests with Student's t‐test. p values <0.05 indicated in bold typeface, p values ≥0.05 indicated in italic typeface. N = 9 mice per genotype. KO, knockout; WT, wild‐type.

Fig 5: Mical1 fl/fl , Tyr::Cre mutant female mice had increased swing times. (A) The percentage of time spent during a single stride in which feet were not in contact with the treadmill surface (swing position) for all four legs for all mice (N = 40 wild‐type (WT) mice, N = 44 Mical1 knockout [KO] mice). For each foot, results are presented for the (B) front left, (C) front right, (D) rear left, (E) rear right swing positions. Graphs indicate means ± standard deviation. Statistical tests with Student's t‐test. p values <0.05 indicated in bold typeface, p values ≥0.05 indicated in italic typeface. N = 10 WT mice, N = 11 Mical1 KO mice.