Vinculin (E1E9V) XP ® Rabbit mAb (HRP Conjugate) 18799S from Cell Signaling Technology

Product Specs
ItemVinculin (E1E9V) XP ® Rabbit mAb (HRP Conjugate)
CompanyCell Signaling Technology
Price $450.00 Inquire
Catalog Number18799S
Molecular Weight124
Research AreaAdhesion/ECM
ReactivityHu, Ms, Rt, Mk
ClonalityMonoclonal
NCBI Gene AliasesCMD1W, CMH15, HEL114, MV, MVCL
NCBI Full Gene Namevinculin
ApplicationsWestern Blot (WB)
HostRabbit
Targetvinculin
Conjugate/TagHRP
Quantity100 µl

Questions and Answers

Can this vinculin antibody be used as a loading control in Western blot experiments?

Yes, the Vinculin (E1E9V) XP® Rabbit mAb (HRP Conjugate) has been utilized as a loading control in various Western blot analyses, as indicated in multiple studies, including those by Li et al. (2023) and Meijer et al. (2021).

What species can this antibody effectively detect?

The Vinculin antibody is reactive with several species, including human, mouse, rat, and non-human primates, making it versatile for studies involving these organisms.

Are there any specific applications where this antibody has been shown to be effective?

This antibody has been demonstrated to work effectively in Western blot applications, as cited in numerous scientific articles, including those by Nofal et al. (2022) and Wang et al. (2019), where it was used to analyze protein expression levels.

How does this antibody perform in studies related to cell adhesion or extracellular matrix interactions?

The Vinculin antibody is relevant in research areas focused on adhesion and the extracellular matrix, as vinculin plays a critical role in cell adhesion processes. Its application in studies, such as those by Chang et al. (2024), highlights its importance in understanding cellular interactions and signaling pathways.

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(1) Rewiring Oncogenic Transcriptional Complexes with Domain-ALTeration Chimeras (DALTACs) in Prostate CancerbioRxivMay 5, 2026Jie Luo, Jianzhang Yang, Jean Ching-Yi Tien, Mi Wang, Sumit Das, Weiguo Xiang, Eleanor Young, Jelena Tosovic, Rahul Mannan, Jocelyn Cai, et al.NKX3–1 (Cell Signaling Technology:83700S); CCND1 (Abcam: ab16663); Vinculin (Cell Signaling Technology: 18799S); GAPDH (Santa Cruz Biotechnology: sc-47724); BRD4 (Bethyl Laboratories: A700–004CF), HA-Tag (Cell Signaling Technology

(2) Targeting the ferritinophagy-lysosome axis as a therapeutic vulnerability in gastroenteropancreatic neuroendocrine tumorsCell Reports MedicineApril 21, 2026Yizhi Cao, Caleb Cheng, Yitong Yin, Sarah N. Yee, Yang Zheng, Somnath Mahapatra, Radha Paturu, Andrej Coleski, Shannon VanAken, Fan Yang, et al.ab19862; RRID: AB_445179 Vinculin (E1E9V) Rabbit mAb (HRP Conjugate),Cell Signaling Technology,Cat# 18799; RRID: AB_2714181 mTOR Antibody,Cell Signaling Technology,Cat# 2972; RRID: AB_330978 Phospho-mTOR (Ser2481) Antibody

(3) Inhibition of adipocyte RUNX1/2 enhances adipose tissue thermogenesis through distinct mechanismsNature CommunicationsApril 2, 2026Cuihua Wang, Na He, Shixiang Wang, Miao Lei, Jie Yao, Li Lin, Xue Ding, Jiaman Lin, Yixin Chen, Qiaoyun Long, et al.rabbit anti-p-AKT (S473) (CST, 4060S), mouse anti-HDAC1 (Abcam, ab7028), mouse anti-VINCULIN (CST, 18799S) and mouse anti-OXPHOS (Abcam, ab110413), rabbit anti-CD45 (Abmart, PA6232S), rabbit anti-Adiponectin

(4) Human iPSC-derived alveolar macrophages reveal macrophage subtype functions of itaconate in M. tuberculosis defenseJCI InsightMarch 9, 2026Adam S. Krebs, Tomi Lazarov, Anthony T. Reynolds, Kimberly A. Dill-McFarland, Abigail Xie, James M. Bean, Muxue Du, Olivier Levy, John A. Buglino, Aaron Zhong, et al.(Cell Signaling Technology, 77510S), HRP-conjugated human anti-vinculin (Cell Signaling Technology, 18799S), and IgG (H+L) goat anti-rabbit HRP (Invitrogen-Fisher, 656120). The following cytokines were used:

(5) Loss of Payload Sensitivity and Other Mechanisms of Resistance to T-DXd in HER2-Mutant NSCLC: Implications for Subsequent Responsiveness to HER2 TKIsJournal of Thoracic OncologyJanuary 16, 2026Monique B. Nilsson, Xiuning Le, Alissa Poteete, Xiaoxing Yu, Junqin He, Qian Huang, Yuji Shibata, Ximeng Liu, Cesar Moran, Ash A. Alizadeh, et al.(Cell Signaling, 86298; RRID:AB_2715541), MRP1 (Cell Signaling, 72202; RRID:AB_2799816), vinculin (Cell Signaling 18799; RRID:AB_2714181), GAPDH (Cell Signaling, 3683; RRID:AB_1642205), SLFN11 (Cell Signaling 34858; RRID:AB_2799063).

(6) GATOR1 complex controls cisplatin sensitivityCell Death & DiseaseDecember 30, 2025Zhenrui Pan, Hanxiao Zhang, Xia Xiao, Catherine Brenner, Svetlana Dokudovskaya(2662), anti-p21 (2946), anti-LC3B (2775), anti-caspase 3 (9662), anti-GAPDH (2118), anti-vinculin (18799) were from Cell Signaling Technology. Anti-PAR antibody (ALX-804-220-R100) was from Enzo Life Sciences.

(7) Elevated cortisol concentration in preterm sheep fetuses impacts heart developmentExperimental PhysiologyDecember 1, 2025Reza Amanollahi, Stacey L. Holman, Melanie R. Bertossa, Ashley S. Meakin, Vicki L. Clifton, Kent L. Thornburg, I. Caroline McMillen, Michael D. Wiese, Mitchell C. Lock, Janna L. Morrisonabundance of target proteins was then normalized to a reference protein, Vinculin (1:2000, cat. no. 18799S, Cell Signaling Technology). 2.7 Quantification of fetal cardiac concentration of glucocorticoid and

(8) Tau Axonal Sorting and Interaction With Synaptic Plasticity Modulators Is Domain- and Isoform-Dependent in Human iPSC-Derived NeuronsAging cellNovember 1, 2025Bell-Simons M, Breuer H, Wunderlich L, Chmes H, Adam D, Klimek J, Buchholz S, Zempel H.(Bio‐Rad). For normalization, signals from HRP‐coupled anti‐GAPDH (ab9385, Abcam) and anti‐vinculin (#18799, Cell Signaling) antibodies were detected. 2.4 Imaging Analysis 2.4.1 Fixation and Immunostaining Neuron

(9) Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancerNat GenetOctober 3, 2025Jie Luo, Zhixiang Chen, Yuanyuan Qiao, Jean Ching-Yi Tien, Eleanor Young, Rahul Mannan, Somnath Mahapatra, Rupam Bhattacharyya, Lanbo Xiao, Tongchen He, et al.FOXA1 (1:1,000; Thermo Fisher Scientific, PA5-27157); Vinculin (1:1,000; Cell Signaling Technology, 18799S); GAPDH (1:1,000; Santa Cruz Biotechnology, sc-47724); BRD2 (1:1,000; Bethyl Laboratories, A700-008);

(10) Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in miceNature CommunicationsSeptember 30, 2025Xun Wu, Ziyi Wang, Katherine R. Croce, Fang Li, Jian Cui, Vivette D. D’Agati, Rajesh K. Soni, Ira Tabas, Ai Yamamoto, Hanrui Zhang(Cell Signaling Technology, 5125S, 1:2000), and HRP-conjugated rabbit-anti-Vinculin (Cell Signaling Technology, 18799S, 1:2000). Target protein and the respective housekeeping protein are derived from the same membrane. T cell

(11) CDK12/13 inactivation triggers STING-mediated antitumor immunity in preclinical modelsJournal of Clinical InvestigationSeptember 16, 2025Yi Bao, Yu Chang, Jean Ching-Yi Tien, Gabriel Cruz, Fan Yang, Rahul Mannan, Somnath Mahapatra, Radha Paturu, Xuhong Cao, Fengyun Su, et al.(Cell Signaling Technology, 4302S), anti–p-Ser2 (Cell Signaling Technology, 13499), anti-vinculin (Cell Signaling Technology, 18799S), anti–mouse p-STING (Cell Signaling Technology, 72971S), anti–human p-STING (Cell Signaling Technology,

(12) Antenatal betamethasone impairs markers of cardiac development and function in near‐term lambsExperimental PhysiologySeptember 4, 2025Reza Amanollahi, Ashley S. Meakin, Stacey L. Holman, Vicki L. Clifton, Kent L. Thornburg, Michael D. Wiese, Kathryn L. Gatford, Mitchell C. Lock, Janna L. MorrisonHealthcare). The reference protein on each membrane was either Vinculin (stained at 1:2000, cat. no. 18799S, Cell Signaling Technology) or β‐tubulin (stained at 1:1000, cat. no. 5346, Cell Signaling Technology)

(13) Antenatal betamethasone impairs markers of cardiac development and function in near‐term lambsExp PhysiolSeptember 4, 2025Reza Amanollahi, Ashley S. Meakin, Stacey L. Holman, Vicki L. Clifton, Kent L. Thornburg, Michael D. Wiese, Kathryn L. Gatford, Mitchell C. Lock, Janna L. MorrisonHealthcare). The reference protein on each membrane was either Vinculin (stained at 1:2000, cat. no. 18799S, Cell Signaling Technology) or β‐tubulin (stained at 1:1000, cat. no. 5346, Cell Signaling Technology)

(14) Concurrent genetic and non-genetic resistance mechanisms to KRAS inhibition in CRCbioRxivAugust 5, 2025Salvador Alonso, Kevan Chu, Marie J Parsons, Elizabeth Granowsky, Himari Gunasinghe, Jinru Shia, Rona Yaeger, Lukas E. Dowused for Western blot analysis were: anti-TBK1 (Cell Signaling 3504), anti-Vinculin (Cell Signaling 18799). KRAS copy number assay Organoids were dissociated and pelleted after three days in culture. Following

(15) Genetic mechanisms of resistance to targeted KRAS inhibitionbioRxivAugust 4, 2025Bianca J. Diaz, Max Kops, Sara Bernardo, Henri Schmidt, Elizabeth Grankowsky, Adrian Vega, Chen Zhang, Matthew Bott, Maria Skamagki, Aidan Tomlinson, et al.for western blot analysis. Antibodies used were B-actin (Abcam ab49900), Vinculin (Cell Signaling #18799), p-Akt (Cell Signaling #4060), p—p44/42 MAPK (Cell Signaling #4370), p-S6 (Cell Signaling #4858), Akt

(16) CDK12/13 inactivation triggers STING-mediated antitumor immunity in preclinical modelsJ Clin InvestJuly 22, 2025Yi Bao, Yu Chang, Jean Ching-Yi Tien, Gabriel Cruz, Fan Yang, Rahul Mannan, Somnath Mahapatra, Radha Paturu, Xuhong Cao, Fengyun Su, et al.(Cell Signaling Technology, 4302S), anti–p-Ser2 (Cell Signaling Technology, 13499), anti-vinculin (Cell Signaling Technology, 18799S), anti–mouse p-STING (Cell Signaling Technology, 72971S), anti–human p-STING (Cell Signaling Technology,

(17) Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editingSci AdvJuly 18, 2025Gemma Valcárcel, Aleksey Lazarenkov, Anna V. López-Rubio, Clara Berenguer, Josep Calafell-Segura, Javier Rodríguez-Ubreva, Esteban Ballestar, José Luis Sardinaanti-β actin (MERCK, catalog no. A1978) and rabbit anti-vinculin (Cell Signaling Technology, catalog no. 18799) 1:5000 in blocking solution. The membranes were washed three times in tris-buffered saline–Tween before

(18) FAF2 is a bifunctional regulator of peroxisomal homeostasis and saturated lipid responsesSci AdvJuly 4, 2025Choah Kim, Katlyn R. Gabriel, Dylan Boone, Matthew R. Brown, Katherine Oppenheimer, Maria Kost-Alimova, Juan Lorenzo B. Pablo, Anna GrekaAntibody,Source XBP1s,"CST, D2C1F" Vinculin-HRP,"CST, 18799S" FAF2,"ABIN, 2856552" Rabbit secondary,"CST, 7074P2" Mouse secondary,"CST, 7076P2" PEX3,"ABclonal,

(19) In Vitro Embryo Culture Impacts Heart Mitochondria in Male Adolescent SheepJ Dev BiolMay 13, 2025Reza Amanollahi, Stacey L. Holman, Ashley S. Meakin, Monalisa Padhee, Kimberley J. Botting-Lawford, Song Zhang, Severence M. MacLaughlin, David O. Kleemann, Simon K. Walker, Jennifer M. Kelly, et al.proteins was adjusted relative to Ponceau S staining or specific reference proteins, vinculin (1:2000, #18799S, Cell Signaling Technology) or β-tubulin (1:1000, # 5346, Cell Signaling Technology). 2.6. Quantification

(20) Elevated cortisol concentration in preterm sheep fetuses impacts heart developmentExp PhysiolApril 28, 2025Reza Amanollahi, Stacey L. Holman, Melanie R. Bertossa, Ashley S. Meakin, Vicki L. Clifton, Kent L. Thornburg, I. Caroline McMillen, Michael D. Wiese, Mitchell C. Lock, Janna L. Morrisonabundance of target proteins was then normalized to a reference protein, Vinculin (1:2000, cat. no. 18799S, Cell Signaling Technology). 2.7 Quantification of fetal cardiac concentration of glucocorticoid and

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3/10/2016Decent Vinvulin Antibody A MOur lab uses Vinculin as a loading control in a number of our western blots, and we usually buy our antibodies from Cell Signaling because of their great reputation.

Vinculin (E1E9V) XP ® Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Vinculin (E1E9V) XP ® Rabbit mAb (HRP Conjugate) from Cell Signaling Technology

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