APC/Cyanine7 anti-mouse CD3 100222 from BioLegend

Product Specs
ItemAPC/Cyanine7 anti-mouse CD3
CompanyBioLegend
Price $242.00Supplier Page View Company Product Page
Catalog Number100222
NCBI Gene AliasesCtg-3, Ctg3, T3g
HostRat
ReactivityMouse
Clone17A2
Quantity100 µg
ApplicationsFC - Quality tested
IsotypeRat IgG2b, kappa
Conjugate/TagAPC/Cyanine7
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
Antibody TypePrimary
ClonalityMonoclonal
TargetCD3

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Website: biolegend.com

(1) BC02-adjuvanted varicella-zoster virus glycoprotein E subunit vaccine overcomes immunosenescence to induce robust neutralizing antibodies and multifunctional T-cell immunity in seropositive aged murine modelsHuman Vaccines & ImmunotherapeuticsDecember 31, 2026Junli Li, Xiaochi Li, Yang Yang, LiLi Fu, Weixin Du, Jiazheng Wei, Kexin Su, Cheng Su, Xiaobing Shen, Guozhi Wang, et al.(Alpha Diagnostic IGGRP-50). Flow cytometry antibodies were purchased from BioLegend: CD3-APC/Cy7 (100222), CD4-FITC (100510), CD8-PE (100708), CD44-PE-Cy7 (103030), CD62L-PE (104408), CD19-APC (115512), and

(2) HE4 drives PD-L1 expression in myeloid cells via IFN-γR-JAK-STAT3 signaling to promote tumor immune evasionCell Reports MedicineApril 21, 2026Bo Zeng, Yingying Zhou, Heping Wang, Panyin Shu, Ting Pan, Fangfang Liu, Yangyang Li, Ming Yi, Ruirui He, Lingyun Feng, et al.anti-mouse CD45,BioLegend,Cat# 157214; RRID: AB_2894427 APC-Cy7-conjugated anti-mouse CD3,BioLegend,Cat# 100221; RRID: AB_2057374 PE-conjugated anti-mouse CD8,BioLegend,Cat# 126607; RRID: AB_961300 PerCP/Cyanine5.5-conjugated

(3) Platelet DKK1 promotes tolerogenic dendritic cells and non-healing responses in cutaneous leishmaniasisiScienceApril 17, 2026Olivia C. Ihedioha, Anutr Sivakoses, Haley Q. Marcarian, Malini Sajeev, Diane McMahon-Pratt, Alfred L.M. Bothwellantibody,eBioscience,Cat#12004182; RRID: AB_465506 APC-Cy7 rat monoclonal anti- mouse CD3 antibody,Biolegend,Cat#100222; RRID: AB_2242784 FITC rat monoclonal anti-mouse CD8 antibody,eBioscience,Cat#11008182; RRID: AB_464915 APC

(4) UHRF1 drives subtype-independent aggressiveness and immune evasion in small cell lung cancer through PRC2 interactionsiScienceApril 17, 2026Yijun Gu, Jamie-Jean De La Torre, Yasamin Fazeli, J. Mateo Valenzuela, Frederick Guzman, Roberto Tinoco, Claudia A. BenaventePE/Cyanine7 anti-mouse F4/80,Biolegend,123114 APC anti-mouse Ly-6C,Biolegend,128016 APC/Cyanine7 anti-mouse CD3,Biolegend,100222 APC/Cyanine7 anti-mouse NK-1.1,Biolegend,156510 BV 421™ anti-mouse I-A/I-E,Biolegend,107631 Brilliant Violet 605™ anti-mouse/human CD11b

(5) The DBC1-HIF-1α-PPAR-γ axis regulates Treg cell differentiation to promote myocardial fibrosis in experimental diabetic cardiomyopathy through the paracrine secretion of AregFront Endocrinol (Lausanne)April 13, 2026Linhe Lu, Yifei Xu, Changnuan Wen, Qiancong Zhao, Zhihang Li, Jiaqi Liu, Fujie Xu, Jincheng Liu, Jiayou Tang30 min at 4 °C in the dark: Brilliant Violet 510-anti-CD45 (#103138, BioLegend), APC-Cy7-anti-CD3 (#100222, BioLegend), FITC-anti-CD4 (#100406, BioLegend), and APC-anti-ST2 (#17-9335-82, Invitrogen, 1:100). Intracellular

(6) Sustained interferon exposure creates a hyper-metastatic subset of melanoma cellsbioRxivApril 5, 2026Mark H. Mannino, Tao Wei, Daniel Cassidy, Benjamin Knight, Devon L. Moose, Adrienne Shannon, Elena Piskounova, Zhiyu Zhao, Sean J. MorrisonBioLegend 114308), CD115 (BV510, Biolegend 750893), CD88 (APC, Biolegend 135808), CD3 (APC-Cy7, Biolegend 100222), CD8a (Brilliant Violet 510, BioLegend 100738), CD4 (BV421, BioLegend 100559), NK1.1 (AF700, BioLegend

(7) IDH1 Mutant Glioma Favors Group 3 Innate Lymphoid Cells and Is Resistant to Immune Checkpoint ExpressionInflamm ResApril 1, 2026Serife Erdem, Yesim Haliloglu, Inayet Nur Uslu, Mohammad Houran, Halil Ulutabanca, Alperen Vural, Mehmet Berat Erturhan, Halit Canatan, Ahmet Ekenwith Isotype Control (BioLegend Cat# 133301), APC/Cyanine7-conjugated anti-mouse CD3 (BioLegend Cat# 100221), PE-conjugated anti-mouse CD45 recombinant antibody (BioLegend Cat# 157603), and PE/Cyanine7-conjugated

(8) Chemoradiation Reprograms Tumor Cells and the Immune Microenvironment in Cervical CancerCancer ResMarch 20, 2026Tito A. Sandoval, Yize Li, Naoshad Muhammad, Tresha Desai, Reyka G. Jayasinghe, Saman Zeeshan, Kay Jayachandran, Matthew J. Inkman, Michael R Waters, Eliwaza Naomi S Msengi, et al.anti-CD45 (30-F11, 1:200, BioLegend Cat# 103128, RRID:AB_493715), anti-CD3 (17A2, 1:200, BioLegend Cat# 100222, RRID:AB_2242784), anti-CD4 (RM4-5, 1:200, BioLegend Cat# 100548, RRID:AB_2563054), anti-CD8α (53-6.7,

(9) Guominkang formula alleviates airway inflammation in HDM-induced asthma mice by regulating Wnt/β-Catenin pathwayChin MedMarch 17, 2026Yuhan Zong, Jingwei Kong, Fan Yang, Manting Wang, Ji Wang, Qi WangSolarbio Science & Technology Co., Ltd. Antibodies from BioLegend, Inc. included APC/Cyanine7 anti-CD3 (100222), APC/Cyanine7 anti-CD4 (100414), APC anti-CD4 (100516), PerCP anti-CD8a (100732), Pacific Blue anti-CD11b

(10) Therapeutic optimization of LIPA targeting to induce endoplasmic reticulum stress and cell death in ovarian cancerOncogeneMarch 10, 2026Suryavathi Viswanadhapalli, Tae-Kyung Lee, Scott Elmore, Gaurav Sharma, Rahul Gopalam, Durga Meenakshi Panneerdoss, Xihui Liu, Karla Parra, Tanner Reese, Michael Hsieh, et al.and fluorophore-labeled mAbs to surface markers, including CD3 (APC-Cy7, Clone 17A2; BioLegend, no. 100221), IgD (FITC, Clone 11-26 c.2a; BioLegend, no. 405704), CD138 (PE-Cy7, Clone 281-2; BioLegend, no. 142514),

(11) The Arp2/3 complex controls the development of homeostatic microgliaEMBO ReportsFebruary 27, 2026Shima Safaiyan, Maximilian Frosch, Tom Bickel, Gianni Monaco, Roie Dvir, Christian Madry, Lance Fredrick Pahutan Bosch, Katrin Kierdorf, Metello Innocenti, Josef Priller, et al.(PE, M1/70)",Thermo Fisher Scientific,Cat#25-0112-82 "Rat anti-CD3 (APC/Cy7, 17/A2)",BioLegend,Cat#100222 "Rat anti-Ly6G (APC/Cy7, 1A8)",BioLegend,Cat#127624 "Rat anti-CD19 (APC/Cy7, 6D5)",BioLegend,Cat#115530 "Rat

(12) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyun141703), BV421-CD86 (BioLegend, 105031), AlexaFluor700-CD45 (BioLegend,147716), APC/Cy7-CD3 (BioLegend, 100222), BV421-CD4 (BioLegend, 100437), BV510-Nkp46 (BioLegend, 137623), BV605-CD8 (BioLegend, 100744), FITC-Ki-67

(13) CD147 regulates CD8+ T cell cytotoxic activity by facilitating phosphorylation and subsequent recruitment of proximal TCR signaling kinasesiScienceFebruary 20, 2026Taiyu Shu, Dan Wang, Hao Wang, Jie Zou, Heyao Qin, Xue Liang, Hongyong Cui, Qian He, Yuxi Zhang, Jianli JiangPerCP/Cyanine5.5 anti-human CD69,BioLegend,Cat# 310926; RRID: AB_2074956 APC/Cyanine7 anti-mouse CD3,Biolegend,Cat# 100222; RRID: AB_2242784 PerCP/Cyanine5.5 anti-mouse CD8a,Biolegend,Cat# 100734; RRID: AB_2075238 APC anti-human CD366 (Tim-3

(14) Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancerCell Reports MedicineFebruary 17, 2026Xiang Yu, Yingxin Ren, Jinlong Luo, Jiaqiang Jiang, Lingzhi Wang, Xinyuan Mao, Yutong Wang, Lang Xie, Yijie Xi, Huilin Huang, et al.RRID:AB_2860726 BV421 CD3 Clone 17A2,Biolegend,Cat# 100228; RRID:AB_2562553 APC-Cy7 CD3 Clone 17A2,Biolegend,Cat# 100222; RRID:AB_2242784 PE CD4 Clone GK1.5,Biolegend,Cat# 100408; RRID:AB_312693 FITC CD8 Clone 53-6.7,Biolegend,Cat#

(15) Single-cell transcriptome sequencing reveals immunological mechanisms by which recombinant Echinococcus granulosus P29 protein alleviates airway inflammation in mice with allergic asthmaParasites & VectorsFebruary 11, 2026Zhichao Zhou, Jianwen Wu, Leiji Fu, Rou Wen, Xiaomin Zhang, Zexin Dang, Junyou Wu, Sijia Bao, Wenxuan Li, Mei Yin, et al.USA), APC-anti-mouse-CD170 (cat. no. 155508, S17007L, BioLegend, USA), APC-Cy7-anti-mouse-CD3 (cat. no. 100222, 17A2, BioLegend, USA), FITC-anti-mouse-CD4 (cat. no. 100406, Gk1.5, BioLegend, USA), APC-anti-mouse-IFN-γ

(16) A montmorillonite-based oral fermentation system enables long-lasting in-situ biosynthesis to restore intestinal homeostasisNature CommunicationsFebruary 6, 2026Yifan Zhang, Zhenzhen Fu, Yanmei Chen, Ju Chen, Huan Chen, Mengmeng Zhang, Yan Pang, Lu Wang, Shunpeng Xing, Sisi Lin, et al.CD86-PE/Cyanine7 (PE Cy7, 105014) CD206-PE-Cy7 (141719), CD206-Alexa Fluor® 647 (AF647, 141712), CD3-APC Cy7 (100221), CD45R/B220-PE-Cy7 (103221), and CD80-PE (104707) were purchased from Biolegend. Con A(C21402) and

(17) Biomimetic Nanomedicine for Senescence‐Modulated Immune Activation Enhances Immunotherapy Efficacy in Hepatocellular CarcinomaAdvanced ScienceFebruary 1, 2026Shiji Fang, Liyun Zheng, Bin Lin, Jiale Chen, Dehai Hou, Yiming Ding, Mengzhu Han, Pan Qin, Mengyuan Wang, Xiaoju Guo, et al.(123108), anti‐CD86 (159204), anti‐CD206 (141708), anti‐CD11c (117306), anti‐CD80 (104714), anti‐CD3 (100222), anti‐CD4 (100412), anti‐CD8a (100708), anti‐FOXP3 (126404), anti‐IFN‐γ (163514), and anti‐NK1.1 (156526)

(18) Stenotrophomonas promotes gastrointestinal tumor progression via STING degradation in tumour cells and mitigated immune responseNature CommunicationsJanuary 26, 2026Huolun Feng, Kunling Chen, Zhihui Xi, Baijin Xia, Xi Chen, Yiqin Huang, Zuda Huang, Jieqing Guo, Dandan Zhu, Fa Ling, et al.(cat: 123141, BioLegend), FITC anti-mouse NK-1.1 (cat:108706, BioLegend), APC/Cy7 anti-mouse CD3 (cat: 100221. BioLegend), PE/Cy7 anti-mouse CD4 (cat: 100422, BioLegend), Alexa Fluor700 Rat Anti-Mouse CD8a (cat:557959,

(19) RAF-independent MEK mutations drive refractory histiocytic neoplasms but respond to ERK inhibitionCancer CellJanuary 12, 2026Eli L. Diamond, Jean-Francois Emile, Takeshi Fujino, Julien Haroche, Maxim I. Maron, Alexander M. Lewis, Jahan Rahman, Anne S. Reiner, Dana Bossert, Marc Rosenblum, et al.anti-CD45.1-Alexa Fluor 700 (clone A20; BioLegend; 110724: 1:500), anti-CD3-APC/Cy7 (clone 17A2; BioLegend; 100222; 1:500), anti-Ly-6A/E (Sca-1)-BV605 (clone D2; BioLegend; 108134; 1:500), anti-CD11b-BV711 (clone M1/70;

(20) Nanoparticle Vaccines Based on the Truncated VZV gE Elicit a Robust Immune Response in MiceVaccines (Basel)January 7, 2026Tianxin Shi, Hai Li, Jiehui Wu, Hongqiao Hu, Jie Jiang, Ruichen Wang, Ziyi Li, Qianqian Cui, Shihong Fu, Kai Nie, et al.1 μL each of CD45 FITC (BioLegend, cat. #103108, San Diego, CA, USA), CD3 APC-Cy7 (BioLegend, cat. #100222, San Diego, CA, USA), CD4 PerCP-Cy5.5 (BioLegend, cat. # 100433, San Diego, CA, USA), CD8 APC (BioLegend,

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11/1/2022APC/Cyanine7 Anti-Mouse CD3 Antibody Maria GonzalezIn our lab we study mouse Natural Killer cells. We use this antibody to create a 'dump' gate which includes CD3, CD19 and TCRb to gate out T-cells.

APC/Cyanine7 anti-mouse CD3 from BioLegend

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APC/Cyanine7 anti-mouse CD3 from BioLegend

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