Human MITF Antibody from R&D Systems, a Bio-Techne Brand

Yes, the MITF antibody has been shown to work effectively in immunostaining applications, as demonstrated in studies investigating the role of β-catenin in melanocyte specification. Specifically, it was used to stain for MITF in whole-mount immunostaining of mouse embryos, highlighting its relevance in understanding melanocyte differentiation processes (colombo2022).

The MITF antibody has been cited in studies involving human cell lines, indicating its effectiveness in detecting human MITF. For instance, it was utilized in research examining the plasticity of melanoma cells, where it contributed to understanding the differentiation states of these cells (comandan2022).

The MITF antibody is versatile and can be used in various applications, including Western blotting, immunocytochemistry, immunofluorescence, and immunohistochemistry. It has been successfully employed in studies that require precise detection of MITF in different experimental setups, such as in the differentiation of neural crest cells (gomez2019-1).

When using the MITF antibody, it is recommended to follow specific dilution protocols as indicated in the literature. For example, in one study, the antibody was used at a dilution of 1:100 for immunostaining, which is critical for achieving optimal results in detecting MITF expression (kaucka2021).