MyBioSource.com's MOUSE ANTI DOG CD25:RPE Antibody is a Mouse monoclonal antibody. It reacts with Canine. This antibody has been shown to work in applications such as: Flow Cytometry.
Description
Mouse anti Dog CD25, clone P4A10 recognises the canine homologue of the human CD25 cell surface antigen, also known at IL-2Ralpha, a glycoprotein of approximately 55kDa expressed primarily by activated T lymphocytes (Abrams et al. 2010). The IL-2 receptor is composed of 3 subunits, an alpha chain (CD25), a beta chain (CD122) and a gamma chain (CD132), CD25 functions as a low affinity receptor for IL-2.Research has demonstrated that antibodies to CD4, FoxP3 and CD25 may be used in studies of T regulatory cells (Tregs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity. Previous studies of canine Tregs have utilized the mouse anti human CD25 monoclonal antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25 (Rissetto et al. 2010). However this clone has poor affinity for canine CD25. The development of clone P4A10 now offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abrams et al. 2010).The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloni et al. 2008), and in genetically related diseases of the hemopoitic system (Bauer et al. 2009). The generation of clone P4A10 provides an improved tool for the identification of canine CD25 and will facilitate studies in this species