RAT ANTI DYKDDDDK TAG:FITC Antibody MBS224499 from MyBioSource.com

Description

Rat anti DYKDDDDK Tag antibody, clone 6F7 recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins. Rat anti DYKDDDDK Tag antibody, clone 6F7 works at neutral pH and has very low

Product Specs
ItemRAT ANTI DYKDDDDK TAG:FITC Antibody
CompanyMyBioSource.com
Price $475.00Supplier Page View Company Product Page
Catalog NumberMBS224499
Quantity0.1 mg
ApplicationsFlow cytometry (FC/FACS), Immunofluorescence (IF)
ConcentrationIgG concentration 0.1mg/ml
Conjugate/TagFITC
FormatFITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
TargetDYKDDDDK TAG
IsotypeIgG1
Clone6F7
HostRat
ClonalityMonoclonal

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Questions and Answers

Can this antibody be used for detecting the DYKDDDDK tag in various applications?

Yes, the Rat anti DYKDDDDK Tag antibody, clone 6F7, is suitable for use in applications such as flow cytometry and immunofluorescence. It recognizes the DYKDDDDK epitope when fused to proteins at either the amino- or carboxyl-terminus or at internal sites.

What is the optimal pH for using this antibody in experiments?

The antibody works effectively at neutral pH, which is important for maintaining the integrity of the epitope and ensuring reliable binding during experimental procedures.

Is this antibody suitable for use in live cell imaging?

While the product description does not explicitly mention live cell imaging, the antibody is conjugated to FITC, a fluorophore that is commonly used in fluorescence microscopy. Its application in immunofluorescence suggests it may be suitable for imaging, but specific protocols for live cell applications should be consulted for optimal results.

What type of specificity does this antibody have regarding the DYKDDDDK tag?

The Rat anti DYKDDDDK Tag antibody, clone 6F7, is a monoclonal antibody that specifically recognizes the hydrophilic octapeptide sequence DYKDDDDK, ensuring high specificity for tagged proteins in various experimental contexts.