MyBioSource.com's RAT ANTI HUMAN G-CSF is a Rat monoclonal antibody. The RAT ANTI HUMAN G-CSF Antibody was generated using CSF3, and G CSF as the antigen. It reacts with Human. This antibody has been shown to work in applications such as: ELISA, and Western Blot.
Description
Rat anti Human G-CSF antibody, clone BVD11-37G10 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919). G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114. Biotinylated Rat anti Human G-CSF antibody, clone BVD11-37G10 has been successfully evaluated as a detection reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-3A5 as a capture reagent and recombinant human G-CSF as antigen