Timeless interacting Protein from MyBioSource.com

Timeless Interacting Protein (TIPIN) is a mammalian protein that interacts with Timeless (TMLS), which plays a role in DNA damage checkpoint responses. Mammalian TIM and its constitutive binding partner, TIPIN (ortholog of S.c. Csm3p, S.p. Swi3p), are replisomeassociated proteins. Both proteins associate with components of the endogenous replication fork complex, and are present at BrdU-positive DNA replication sites. Further, the direct binding of the TIM-Tipin complex to the 34 kDa subunit of replication protein A provides a biochemical explanation for the potential coupling role of these proteins. Like TIM, TIPIN is also involved in the molecular mechanism of UVdependent checkpoint activation and cell growth arrest. TIPIN additionally associates with peroxiredoxin2 and appears to be involved in checkpoint responses to H2O2, a role recently described for yeast versions of TIM and Tipin (1, 2). TIPIN is a nuclear protein that associates with the replicative helicase and protects cells against genotoxic agents. Tipin is required for efficient cell cycle arrest in response to DNA damage, and a reduction in TIPIN makes cells sensitive to ionizing radiation as well as replication stress. Loss of TIPIN causes the cells to become spontaneous gamma-H2AX foci, a marker for DNA double-strand breaks. TIPIN and Timeless form a complex via interacting near the N-terminal motifs that maintains the level of both proteins in cells and that the loss of either one will lead to the loss of the interacting partner (2), suggesting that complex formation is required for stabilization and nuclear accumulation of both proteins. TIPIN is capable of regulating TMLS activity by disrupting the ability of TIPIN to form homo-multimeric complexes, TIMLS also forms a functional complex with TIPIN, and provide a starting point from which to link TIMLS to biochemical pathways controlling vital cellular functions. Mammalian TIPIN has 2 variants of 36kDa proteins, the mouse protein is slightly smaller (278aa) with significant homology to human TIPIN protein. Anti-TIPIN antibodies are affinity purified on immobilized affinity based chromatography and characterized for their specificity for TIPIN on ELISA and Western blotting protocol using Western blot positive control sample. The TIPIN antibodies do not cross react with other genes involved in circadian rythms including per, TIM2, TIMLS or with other cellular proteins. MyBioSource has also produced antibodies to TIMLS protein, for a complete listing please visit www.mybiosource.com. MyBioSource will also provide western blot positive control for TIPIN in ready-to-use buffer for SDS-PAGE and Western blotting experiment. Limited quantities of antigenic blocking peptides are also available (please inquire before placing the orders). MyBioSource., will labels these antibodies to fluorophores and secondary enzymes at nominal cost