Fig 2: The variations of the integrative models for postmitotic assembly pathway.Detailed views of the best and lower-scoring pathways. The side views of the 3 spokes are shown. The NE surface is indicated by wireframe model (grey). The uncertainty of each Nup localization is indicated by the density of the corresponding color: The Y-complex (green), isolated fraction of Nup155 (orange), Nup93-Nup188-Nup155 and Nup205-Nup93-Nup155 complexes (blue), and Nup62-Nup58-Nup54 complex (purple). The posterior model likelihood for each pathway is indicated (see Methods for details). The variations in the structural models for the intermediates at 5 and 6 min after anaphase onset would be due to their lower protein density (lower signal-to-noise ratio) in the electron tomography images.

Fig 3: Postmitotic assembly kinetics of Nup188 is similar to other inner ring components.a, Digital PCR analysis of Nup188-mEGFP genome-edited cells for assessing the homozygous tagging. Allele copy numbers of GFP integrations and on-target GFP integrations are quantified. The data are from 2 and 3 measurements for wild-type and Nup188-mEGFP genome-edited HeLa cells. Error bars represent the s.d. of the mean. b, Immunoblot analysis of Nup188-mEGFP cells using antibodies against Nup188 and γ-Tubulin. Uncropped immunoblot data are shown in Supplementary Fig. 1. c, Calculated copy number of Nup188 per nuclear pore as in Fig. 1. The plot is from 20 cells. The median is depicted as a line. d, Time-lapse 3D imaging of Nup188-mEGFP genome-edited cell. The cell nuclei were stained with silicon–rhodamine (SiR)-DNA. Single confocal sections of SiR and GFP channels are shown. Images were filtered with a median filter (kernel size: 0.25 × 0.25 μm). Scale bar, 10 μm. Time after AO is indicated. e, Postmitotic assembly kinetics of Nup188 was decomposed as in Extended Data Fig. 5. Dots represent the average and s.d. of measurements from 16 cells. The kinetics of Nup107, Nup93 and Nup214 are also shown for comparison. Source data