Prolactin Antibody from MyBioSource.com

Alternate Nomenclature: Growth hormone, GH, Somatotropic hormone, The peptide hormones of the anterior pituitary are essential for growth, reproduction and intermediary metabolism. Their synthesis and secretion is controlled by hormones of hypothalamic origin, diseases and several pharmacological agents. In vertebrates 10 anterior pituitary hormones have been identified and are classified in to three classes based on their structural features. The growth hormone (GH), prolactin (Prl) and placental lactogen (PL) are members of somototropic hormone family. The glycoprotein hormone family comprise of Thyroid stimulating hormone (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH). The corticotrophin (ACTH), two melanocyte stimulating hormones (MSH) and the two lipotropins (LPH) are the members of hormones derived from POMC gene. The human growth hormone (hGH) is coded by one gene of a five gene cluster located on chromosome 17, the other genes code for two other variants of lactogen or a growth hormone variant expressed in placenta. The hGH is a single polypeptide chain with 191 amino acids (22 kDa). The hGH has 2 disulfide bonds and it is not glycosylated. There exist a splice variant of hGH where amino acids 32-46 is not translated and this splice variant accounts up to 5- 10% of the total hGH. Placenta also secrete a 22 kDa hGH that can be distinguished from the pituitary form but its significance is not clear. The amount of GH secreted in 24 hours is high in children reaches maximal levels at the time of adolescence then tapers off during adult hood. The secretion of GH is in pulses and occurs at irregular intervals. The amplitude of GH concentration is maximum at night shortly after onset of REM sleep. Somatotrophs in anterior pituitary have an intracellular Ca+2 oscillations that are believed to to be responsible for pulsatile secretion of GH (1). The release of GH is under direct control of GH release hormone (GHRH) which stimulates GH. Both GH and GHRH bind to their cognate GPCR leading to an increase (GHRH) or decrease (somatotropin) in cAMP and calcium levels. Several pharmacological agents can increase GH release via acting on hypothalamus. The physiological effects of GH is via its interaction with cell surface receptor which are widely distributed throughout body. The GH receptor is a 620 amino acid single trans-membrane protein with large extracellular N-terminal domain that interacts with GH and a about 250 amino acid cytoplasmic end of the protein. The large extracellular domain of GH receptor is cleaved to acts as GH binding protein of about 60kDa. The GH has direct effect on lipid and carbohydrate metabolism, its anabolic growth promoting effects are mediated by somatomedins or insulin like growth factors (IGF-1 and IGF-2) which share significant homology (2). GH administration increase the production of IGF in liver, triglyceride hydrolysis and hepatic glucose output. IGF-1 also exerts indirect growth promoting effects of GH by increase chondrogenesis, skeletal and soft tissue growth. The GH-selective antibodies were generated against peptide form unique regions near of human GH protein. The selected peptide sequences are highly antigenic but unique to GH and are absent in other proteins. These peptide sequences are conserved in several other species. MyBioSource has generated rabbit anti-GH mono-epitope-specific antibodies utilizing linear and cyclic peptide methodology. The Anti-GH antibodies have been fully characterized for cross reactivity with other species GH molecules and for other cellular proteins using Western blot analyses. The GH antibody is also available as FITC-conjugatefor direct applications in IHC, confocal and other applications, other conjugates can be ordered at a nominal charge