MyBioSource.com's Dopamine D2 Receptor is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunoprecipitation, and Western Blot.
Description
Five types of dopamine receptors designated as (dopamine D1-dopamine D5) have been characterized based on cDNA cloning, ligand binding and behavioral responses to antagonist/agonists. Molecular cloning of dopamine receptors have identified a family of closely related genes that encodes 5 proteins with some splice variants exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40- 60 kDa. Individual members of the dopamine receptor family have predicted secondary structure having 7 trans-membrane spanning helical domains characteristic features of G-protein-coupled receptors. Stimulation of dopamine D2 receptor led to Gi coupled adenylate cyclase activation resulting in decrease in cAMP levels. Dopamine D2 receptors are expressed in two splice variant form (D2L and D2S), D2L is the major variant and has an exon expressing 29 amino acids. All dopamine receptors proteins are glycosylated at or near the C-terminus and are present on either cell surface or in intracellular sites. Some transporters exhibit dynamic trafficking between intracellular storage sites and plasma membranes in response to various stimuli. Dopamine receptors are believed to be associated with other proteins including members of other Gprotein coupled receptors, resulting in a significant increase in their apparent molecular weight. The dopamine receptor family-selective antibodies were generated against unique N or C-terminal cyclic peptides characteristics of a particular dopamine receptor. MyBioSource has generated highly specific rabbit antidopamine D2 receptor polyclonal antibodies utilizing unique Nterminal sequences (1). These antibodies have been fully characterized for cross reactivity with in the dopamine receptor family and with other cellular pr