MyBioSource.com's Sestrin 2 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunoprecipitation, and Western Blot. The Sestrin 2 Antibody was generated using SESN1, and sestrin 1 as the antigen and it reacts with Human, Mouse, and Rat.
Description
Sestrin 2(SESN2), located at human chromosome 1p35.3, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Recently it was reported that sestrin 2 is not a sulfinic peroxiredoxin reductase (4). Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy (5). Depletion of sestrin 2 in human cancer cells reduced the level of autophagy when autophagy was induced in these cells (5). Similar to sestrin 1, sestrin 2 negatively regulates the activity of mammalian target of rapamycin (mTOR), a positive regulator of cell growth (6). Sestrin 2 may be a promising target in the treatment of chronic obstructive pulmonary disease (COPD). Mutations in Sestrin 2 causing inactivation of sestrin 2 induced TGF- signaling and partially reduced the emphysema phenotype in mice model of COPD (7). Sestrin 2 might also be a key target in the treatment of cancer. It was shown to activate AMPK and repress mTORC1 signaling. AMPK is an essential mediator of LKB1 tumor suppressor and it is down-regulated in cancer cells (8). The Sestrin 2 promotor and sulfiredoxin acitivity is under epigenetic control via acetylation of histone which contribute to transcriptional activation of gene (9). Sestrin 2 is approximately a 57.6kDa protein based on the amino acid predicted MW of 57kDa (480 amino acids). The SESN2-selective antibodies were generated against synthetic peptides corresponding to the N-terminal region of Sestrin 2 protein. The peptide was covalently modified to achieve all post-translational modifications and conjugated to a carrier protein before using it as antigen to produce antibodies in rabbits. SESN2-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. MyBioSource will also provide limited quantities of antigenic blocking protein for SESN-201AP antibody. Antibodies to SESN2 (SESN-201AP) will label ~57.6kDa protein in Western blot positive control samples for SESN2 and several other tissues. MyBioSource will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost