Fig 1: RNMT-RAM Interacts with Transcription-Associated Complexes(A) Identification of HA-RNMT-interacting proteins using SILAC-based mass spectrometry.(B) Fold enrichment of proteins in HA-RNMT IP/IgG IP plotted against the −log10 posterior error probability score. Transcription-associated proteins are highlighted (with the number of peptides identified in parentheses). Three-fold enrichment over control cutoff.(C–E) HeLa cell extracts subject to IP-western blot analysis for CTR9 (C), RNMT (D), and LEO1 (E). Where indicated, IPs were incubated with or without RNase (C). Anti-glutathione S-transferase (GST) IP, negative control.(F) HeLa cells transfected with pcDNA5 HA-RNMT WT and mutants or vector control for 48 hr. Extracts were subject to CTR9 IP and HA western blot analysis.(G) HeLa cells transfected with RAM siRNA or non-targeting control for 36 hr. CTR9 ChIP was performed. Average and SEM are presented for the PCR signal relative to input (background subtracted) (n ≥ 3).(H) HeLa cells transfected with two RAM siRNAs or sc siRNA for 24 and 36 hr. Western blot analysis is shown.