Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [Alexa Fluor® 488] [NBP2-42225AF488] - U2OS cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD63 Antibody [H5C6] conjugated to Alexa Fluor 488 (NBP2-42225AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
CD63 Antibody (H5C6) [Alexa Fluor® 488]
Supplier Page from Novus Biologicals, a Bio-Techne Brand for CD63 Antibody (H5C6) [Alexa Fluor® 488]
Available conjugates: Allophycocyanin;PerCP;Alexa Fluor 750;Allophycocyanin/Cy7;mFluor Violet 450 SE;Biotin;DyLight 405;Alexa Fluor 350;mFluor Violet 500 SE;Alexa Fluor 405;PE/Atto594;Alexa Fluor 700;DyLight 550;Alexa Fluor 488