(LEFT)Western blot analysis of anti-MST1 Pab in HL60 cell lysate. MST1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT)Western blot analysis of STK4 (arrow) using MST1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK4 gene (Lane 2) (Origene Technologies).