Cyclin B1 Antibody MBS438370 from MyBioSource.com

Product Specs
ItemCyclin B1 Antibody
CompanyMyBioSource.com
Price $275.00Supplier Page View Company Product Page
Catalog NumberMBS438370
Quantity0.1 mg
ApplicationsELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Immunohistology (IH)
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Hamster
TargetCyclin B1
IsotypeIgG1 kappa
Molecular Weight55-62kDa
CloneV92.1
HostMouse
ImmunogenIt recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition
NCBI Full Gene Namecyclin B1
NCBI Gene AliasesCCNB
ClonalityMonoclonal